diff --git a/unilabos/app/main.py b/unilabos/app/main.py
index 9a1d5dc7..e9d2f15b 100644
--- a/unilabos/app/main.py
+++ b/unilabos/app/main.py
@@ -24,7 +24,7 @@ from unilabos.utils.banner_print import print_status, print_unilab_banner
 
 def load_config_from_file(config_path, override_labid=None):
     if config_path is None:
-        config_path = os.environ.get("UNILABOS.BASICCONFIG.CONFIG_PATH", None)
+        config_path = os.environ.get("UNILABOS_BASICCONFIG_CONFIG_PATH", None)
     if config_path:
         if not os.path.exists(config_path):
             print_status(f"配置文件 {config_path} 不存在", "error")
@@ -126,7 +126,7 @@ def parse_args():
         "--labid",
         type=str,
         default="",
-        help="实验室唯一ID，也可通过环境变量 UNILABOS.MQCONFIG.LABID 设置或传入--config设置",
+        help="实验室唯一ID，也可通过环境变量 UNILABOS_MQCONFIG_LABID 设置或传入--config设置",
     )
     return parser
 
diff --git a/unilabos/config/config.py b/unilabos/config/config.py
index 5e85e9e2..44bcc25a 100644
--- a/unilabos/config/config.py
+++ b/unilabos/config/config.py
@@ -109,13 +109,13 @@ def _update_config_from_module(module, override_labid: str):
 
 
 def _update_config_from_env():
-    prefix = "UNILABOS."
+    prefix = "UNILABOS_"
     for env_key, env_value in os.environ.items():
         if not env_key.startswith(prefix):
             continue
         try:
             key_path = env_key[len(prefix):]  # Remove UNILAB_ prefix
-            class_field = key_path.upper().split(".", 1)
+            class_field = key_path.upper().split("_", 1)
             if len(class_field) != 2:
                 logger.warning(f"[ENV] 环境变量格式不正确：{env_key}")
                 continue
@@ -163,7 +163,7 @@ def _update_config_from_env():
 def load_config(config_path=None, override_labid=None):
     # 如果提供了配置文件路径，从该文件导入配置
     if config_path:
-        env_config_path = os.environ.get("UNILABOS.BASICCONFIG.CONFIG_PATH")
+        env_config_path = os.environ.get("UNILABOS_BASICCONFIG_CONFIG_PATH")
         config_path = env_config_path if env_config_path else config_path
         BasicConfig.config_path = os.path.abspath(os.path.dirname(config_path))
         if not os.path.exists(config_path):