mirror of
https://github.com/dptech-corp/Uni-Lab-OS.git
synced 2026-02-05 14:05:12 +00:00
update
This commit is contained in:
Guangxin Zhang
@@ -716,14 +716,14 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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raise ValueError(f"Length of `asp_vols` {len(asp_vols)} must match `targets` {len(targets)}.")
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# 首先应该对任务分组，然后每次1个/8个进行操作处理
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if len(use_channels) == 1:
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tip = []
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for x in range(len(use_channels)):
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tip.extend(next(self.current_tip))
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await self.pick_up_tips(tip)
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for _ in range(len(targets)):
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tip = []
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for x in range(len(use_channels)):
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tip.extend(next(self.current_tip))
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await self.pick_up_tips(tip)
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await self.aspirate(
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resources=reagent_sources,
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resources=[reagent_sources[_]],
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vols=[asp_vols[_]],
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use_channels=use_channels,
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flow_rates=[flow_rates[0]] if flow_rates else None,
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@@ -759,17 +759,18 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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if delays is not None:
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await self.custom_delay(seconds=delays[1])
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await self.touch_tip(targets[_])
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await self.discard_tips()
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await self.discard_tips()
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elif len(use_channels) == 8:
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# 对于8个的情况，需要判断此时任务是不是能被8通道移液站来成功处理
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if len(targets) % 8 != 0:
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raise ValueError(f"Length of `targets` {len(targets)} must be a multiple of 8 for 8-channel mode.")
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tip = []
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for _ in range(len(use_channels)):
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tip.extend(next(self.current_tip))
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await self.pick_up_tips(tip)
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for i in range(0, len(targets), 8):
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tip = []
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for _ in range(len(use_channels)):
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tip.extend(next(self.current_tip))
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await self.pick_up_tips(tip)
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current_targets = targets[i:i + 8]
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current_reagent_sources = reagent_sources[i:i + 8]
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current_asp_vols = asp_vols[i:i + 8]
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@@ -819,7 +820,7 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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if delays is not None:
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await self.custom_delay(seconds=delays[1])
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await self.touch_tip(current_targets)
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await self.discard_tips()
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await self.discard_tips()
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# except Exception as e:
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@@ -977,9 +977,186 @@ if __name__ == "__main__":
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# 4.
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deck = PRCXI9300Deck(name="PRCXI_Deck_9300", size_x=100, size_y=100, size_z=100)
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# deck = PRCXI9300Deck(name="PRCXI_Deck_9300", size_x=100, size_y=100, size_z=100)
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from pylabrobot.resources.opentrons.tip_racks import opentrons_96_tiprack_300ul,opentrons_96_tiprack_10ul
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# from pylabrobot.resources.opentrons.tip_racks import opentrons_96_tiprack_300ul,opentrons_96_tiprack_10ul
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# from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
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# def get_well_container(name: str) -> PRCXI9300Container:
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# well_containers = corning_96_wellplate_360ul_flat(name).serialize()
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# plate = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="plate",
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# ordering=collections.OrderedDict())
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# plate_serialized = plate.serialize()
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# plate_serialized["parent_name"] = deck.name
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# well_containers.update({k: v for k, v in plate_serialized.items() if k not in ["children"]})
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# new_plate: PRCXI9300Container = PRCXI9300Container.deserialize(well_containers)
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# return new_plate
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# def get_tip_rack(name: str) -> PRCXI9300Container:
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# tip_racks = opentrons_96_tiprack_300ul("name").serialize()
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# tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
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# ordering=collections.OrderedDict())
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# tip_rack_serialized = tip_rack.serialize()
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# tip_rack_serialized["parent_name"] = deck.name
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# tip_racks.update({k: v for k, v in tip_rack_serialized.items() if k not in ["children"]})
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# new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
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# return new_tip_rack
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# plate1 = get_tip_rack("RackT1")
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# plate1.load_state({
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# "Material": {
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# "uuid": "076250742950465b9d6ea29a225dfb00",
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# "Code": "ZX-001-300",
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# "Name": "300μL Tip头"
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# }
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# })
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# plate2 = get_well_container("PlateT2")
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# plate2.load_state({
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# "Material": {
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# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
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# "Code": "ZX-019-2.2",
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# "Name": "96深孔板"
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# }
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# })
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# plate3 = PRCXI9300Trash("trash", size_x=50, size_y=100, size_z=10, category="trash")
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# plate3.load_state({
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# "Material": {
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# "uuid": "730067cf07ae43849ddf4034299030e9"
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# }
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# })
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# plate4 = get_well_container("PlateT4")
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# plate4.load_state({
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# "Material": {
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# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
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# "Code": "ZX-019-2.2",
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# "Name": "96深孔板"
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# }
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# })
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# plate5 = get_well_container("PlateT5")
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# plate5.load_state({
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# "Material": {
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# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
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# "Code": "ZX-019-2.2",
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# "Name": "96深孔板"
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# }
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# })
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# plate6 = get_well_container("PlateT6")
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# plate6.load_state({
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# "Material": {
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# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
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# "Code": "ZX-019-2.2",
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# "Name": "96深孔板"
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# }
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# })
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# deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
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# deck.assign_child_resource(plate2, location=Coordinate(0, 0, 0))
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# deck.assign_child_resource(plate3, location=Coordinate(0, 0, 0))
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# deck.assign_child_resource(plate4, location=Coordinate(0, 0, 0))
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# deck.assign_child_resource(plate5, location=Coordinate(0, 0, 0))
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# deck.assign_child_resource(plate6, location=Coordinate(0, 0, 0))
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# # print(plate2)
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# plate_2_liquids = [[('water', 500)]]*96
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# plate2.set_well_liquids(plate_2_liquids)
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# handler = PRCXI9300Handler(deck=deck, host="10.181.214.132", port=9999,
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# timeout=10.0, setup=False, debug=False,
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# matrix_id="71593",
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# channel_num=8, axis="Left") # Initialize the handler with the deck and host settings
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# handler.set_tiprack([plate1])
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# asyncio.run(handler.setup()) # Initialize the handler and setup the connection
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# from pylabrobot.resources import set_volume_tracking
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# # from pylabrobot.resources import set_tip_tracking
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# set_volume_tracking(enabled=True)
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# from unilabos.resources.graphio import *
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# A = tree_to_list([resource_plr_to_ulab(deck)])
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# with open("deck_9300_new.json", "w", encoding="utf-8") as f:
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# json.dump(A, f, indent=4, ensure_ascii=False)
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# asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
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# # asyncio.run(handler.pick_up_tips(plate1.children[:8],[0,1,2,3,4,5,6,7]))
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# # print(plate1.children[:8])
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# # asyncio.run(handler.aspirate(plate2.children[:8],[50]*8, [0,1,2,3,4,5,6,7]))
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# # print(plate2.children[:8])
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# # asyncio.run(handler.dispense(plate5.children[:8],[50]*8,[0,1,2,3,4,5,6,7]))
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# # print(plate5.children[:8])
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# # # # # asyncio.run(handler.drop_tips(tip_rack.children[8:16],[0,1,2,3,4,5,6,7]))
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# # asyncio.run(handler.discard_tips())
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# # asyncio.run(handler.mix(well_containers.children[:8
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# # ], mix_time=3, mix_vol=50, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
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# # #print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2)) # Print matrix info
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# # asyncio.run(handler.add_liquid(
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# # asp_vols=[100]*16,
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# # dis_vols=[100]*16,
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# # reagent_sources=plate2.children[:16],
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# # targets=plate5.children[:16],
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# # use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
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# # flow_rates=[None] * 32,
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# # offsets=[Coordinate(0, 0, 0)] * 32,
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# # liquid_height=[None] * 16,
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# # blow_out_air_volume=[None] * 16,
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# # delays=None,
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# # mix_time=3,
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# # mix_vol=50,
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# # spread="wide",
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# # ))
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# # asyncio.run(handler.run_protocol()) # Run the protocol
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# # asyncio.run(handler.remove_liquid(
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# # vols=[100]*16,
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# # sources=plate2.children[-16:],
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# # waste_liquid=plate5.children[:16], # 这个有些奇怪，但是好像也只能这么写
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# # use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
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# # flow_rates=[None] * 32,
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# # offsets=[Coordinate(0, 0, 0)] * 32,
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# # liquid_height=[None] * 32,
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# # blow_out_air_volume=[None] * 32,
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# # spread="wide",
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# # ))
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# acid = [20]*8+[40]*8+[60]*8+[80]*8+[100]*8+[120]*8+[140]*8+[160]*8+[180]*8+[200]*8+[220]*8+[240]*8
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# alkaline = acid[::-1] # Reverse the acid list for alkaline
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# asyncio.run(handler.transfer_liquid(
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# asp_vols=acid,
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# dis_vols=acid,
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# tip_racks=[plate1],
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# sources=plate2.children[:],
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# targets=plate5.children[:],
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# use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
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# offsets=[Coordinate(0, 0, 0)] * 32,
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# asp_flow_rates=[None] * 16,
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# dis_flow_rates=[None] * 16,
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# liquid_height=[None] * 32,
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# blow_out_air_volume=[None] * 32,
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# mix_times=3,
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# mix_vol=50,
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# spread="wide",
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# ))
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# asyncio.run(handler.run_protocol()) # Run the protocol
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# # # input("Running protocol...")
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# # # input("Press Enter to continue...") # Wait for user input before proceeding
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# # # print("PRCXI9300Handler initialized with deck and host settings.")
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# # Example usage
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# # 1. 用导出的json，给每个T1 T2板子设定相应的物料，如果是孔板和枪头盒，要对应区分
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# # 2. 设计一个单点动作流程，可以跑
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# # 3.
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deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
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from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul,opentrons_96_tiprack_10ul
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from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
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def get_well_container(name: str) -> PRCXI9300Container:
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@@ -993,7 +1170,7 @@ if __name__ == "__main__":
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return new_plate
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def get_tip_rack(name: str) -> PRCXI9300Container:
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tip_racks = opentrons_96_tiprack_300ul("name").serialize()
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tip_racks = opentrons_96_tiprack_10ul("name").serialize()
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tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
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ordering=collections.OrderedDict())
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tip_rack_serialized = tip_rack.serialize()
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@@ -1002,333 +1179,157 @@ if __name__ == "__main__":
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new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
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return new_tip_rack
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plate1 = get_tip_rack("RackT1")
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plate1 = get_well_container("HPLCPlateT1")
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plate1.load_state({
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"Material": {
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"uuid": "076250742950465b9d6ea29a225dfb00",
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"Code": "ZX-001-300",
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"Name": "300μL Tip头"
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"uuid": "548bbc3df0d4447586f2c19d2c0c0c55",
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"Code": "HPLC01",
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"Name": "HPLC料盘"
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}
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})
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plate2 = get_well_container("PlateT2")
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plate2.load_state({
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"Material": {
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"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
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"Code": "ZX-019-2.2",
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"Name": "96深孔板"
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"uuid": "04211a2dc93547fe9bf6121eac533650",
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}
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})
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plate3 = PRCXI9300Trash("trash", size_x=50, size_y=100, size_z=10, category="trash")
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plate3 = get_well_container("PlateT3")
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plate3.load_state({
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"Material": {
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"uuid": "04211a2dc93547fe9bf6121eac533650",
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}
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})
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trash = PRCXI9300Trash(name="trash", size_x=50, size_y=50, size_z=10, category="trash")
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trash.load_state({
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"Material": {
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"uuid": "730067cf07ae43849ddf4034299030e9"
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}
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})
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plate4 = get_well_container("PlateT4")
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plate4.load_state({
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"Material": {
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"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
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"Code": "ZX-019-2.2",
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"Name": "96深孔板"
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}
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})
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plate5 = get_well_container("PlateT5")
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plate5.load_state({
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"Material": {
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"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
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"Code": "ZX-019-2.2",
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"Name": "96深孔板"
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"uuid": "04211a2dc93547fe9bf6121eac533650",
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}
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})
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plate6 = get_well_container("PlateT6")
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plate6.load_state({
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"Material": {
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"uuid": "04211a2dc93547fe9bf6121eac533650"
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}
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})
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plate7 = PRCXI9300Container(name="plateT7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
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plate7.load_state({
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"Material": {
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"uuid": "04211a2dc93547fe9bf6121eac533650"
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}
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})
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plate8 = get_tip_rack("RackT8")
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plate8.load_state({
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"Material": {
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"uuid": "068b3815e36b4a72a59bae017011b29f",
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"Code": "ZX-001-10+",
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"Name": "10μL加长 Tip头"
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}
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})
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plate9 = get_well_container("PlateT9")
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plate9.load_state({
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"Material": {
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"uuid": "04211a2dc93547fe9bf6121eac533650"
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}
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})
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plate10 = get_well_container("PlateT10")
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plate10.load_state({
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"Material": {
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"uuid": "04211a2dc93547fe9bf6121eac533650"
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}
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})
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plate11 = get_well_container("PlateT11")
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plate11.load_state({
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"Material": {
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"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
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"Code": "ZX-019-2.2",
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"Name": "96深孔板"
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}
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})
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plate12 = get_well_container("PlateT12")
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plate12.load_state({
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"Material": {
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"uuid": "04211a2dc93547fe9bf6121eac533650"
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}
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})
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plate13 = get_well_container("PlateT13")
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plate13.load_state({
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"Material": {
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"uuid": "04211a2dc93547fe9bf6121eac533650"
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}
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})
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# container_for_nothing = PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
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deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
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deck.assign_child_resource(plate2, location=Coordinate(0, 0, 0))
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deck.assign_child_resource(plate3, location=Coordinate(0, 0, 0))
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deck.assign_child_resource(plate4, location=Coordinate(0, 0, 0))
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deck.assign_child_resource(plate5, location=Coordinate(0, 0, 0))
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deck.assign_child_resource(plate6, location=Coordinate(0, 0, 0))
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deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing2", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(trash, location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing3", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing4", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(plate8, location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing5", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing6", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(plate11, location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing8", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
|
||||
# print(plate2)
|
||||
plate_2_liquids = [[('water', 500)]]*96
|
||||
plate2.set_well_liquids(plate_2_liquids)
|
||||
|
||||
handler = PRCXI9300Handler(deck=deck, host="10.181.214.132", port=9999,
|
||||
handler = PRCXI9300Handler(deck=deck, host="10.181.102.13", port=9999,
|
||||
timeout=10.0, setup=False, debug=False,
|
||||
matrix_id="71593",
|
||||
channel_num=8, axis="Left") # Initialize the handler with the deck and host settings
|
||||
matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1",
|
||||
channel_num=1, axis="Right") # Initialize the handler with the deck and host settings
|
||||
|
||||
|
||||
handler.set_tiprack([plate1])
|
||||
|
||||
handler.set_tiprack([plate8]) # Set the tip rack for the handler
|
||||
asyncio.run(handler.setup()) # Initialize the handler and setup the connection
|
||||
from pylabrobot.resources import set_volume_tracking
|
||||
# from pylabrobot.resources import set_tip_tracking
|
||||
set_volume_tracking(enabled=True)
|
||||
|
||||
plate11.set_well_liquids([("Water", 100) if (i % 8 == 0 and i // 8 < 6) else (None, 100) for i in range(96)]) # Set liquids for every 8 wells in plate8
|
||||
|
||||
from unilabos.resources.graphio import *
|
||||
|
||||
A = tree_to_list([resource_plr_to_ulab(deck)])
|
||||
with open("deck_9300_new.json", "w", encoding="utf-8") as f:
|
||||
json.dump(A, f, indent=4, ensure_ascii=False)
|
||||
# with open("deck.json", "w", encoding="utf-8") as f:
|
||||
# json.dump(A, f, indent=4, ensure_ascii=False)
|
||||
|
||||
print(plate11.get_well(0).tracker.get_used_volume())
|
||||
asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
|
||||
|
||||
# asyncio.run(handler.pick_up_tips(plate1.children[:8],[0,1,2,3,4,5,6,7]))
|
||||
# print(plate1.children[:8])
|
||||
# asyncio.run(handler.aspirate(plate2.children[:8],[50]*8, [0,1,2,3,4,5,6,7]))
|
||||
# print(plate2.children[:8])
|
||||
# asyncio.run(handler.dispense(plate5.children[:8],[50]*8,[0,1,2,3,4,5,6,7]))
|
||||
# print(plate5.children[:8])
|
||||
|
||||
# # # # asyncio.run(handler.drop_tips(tip_rack.children[8:16],[0,1,2,3,4,5,6,7]))
|
||||
# asyncio.run(handler.pick_up_tips([plate8.children[8]],[0]))
|
||||
# print(plate8.children[8])
|
||||
# # asyncio.run(handler.run_protocol())
|
||||
# asyncio.run(handler.aspirate([plate11.children[0]],[10], [0]))
|
||||
# print(plate11.children[0])
|
||||
# # asyncio.run(handler.run_protocol())
|
||||
# asyncio.run(handler.dispense([plate1.children[0]],[10],[0]))
|
||||
# print(plate1.children[0])
|
||||
# # asyncio.run(handler.run_protocol())
|
||||
# asyncio.run(handler.mix([plate1.children[0]], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
|
||||
# print(plate1.children[0])
|
||||
# asyncio.run(handler.discard_tips())
|
||||
|
||||
# asyncio.run(handler.mix(well_containers.children[:8
|
||||
# ], mix_time=3, mix_vol=50, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
|
||||
# #print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2)) # Print matrix info
|
||||
# asyncio.run(handler.add_liquid(
|
||||
# asp_vols=[100]*16,
|
||||
# dis_vols=[100]*16,
|
||||
# reagent_sources=plate2.children[:16],
|
||||
# targets=plate5.children[:16],
|
||||
# use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
|
||||
# flow_rates=[None] * 32,
|
||||
# offsets=[Coordinate(0, 0, 0)] * 32,
|
||||
# liquid_height=[None] * 16,
|
||||
# blow_out_air_volume=[None] * 16,
|
||||
# delays=None,
|
||||
# mix_time=3,
|
||||
# mix_vol=50,
|
||||
# spread="wide",
|
||||
# ))
|
||||
# asyncio.run(handler.run_protocol()) # Run the protocol
|
||||
# asyncio.run(handler.remove_liquid(
|
||||
# vols=[100]*16,
|
||||
# sources=plate2.children[-16:],
|
||||
# waste_liquid=plate5.children[:16], # 这个有些奇怪，但是好像也只能这么写
|
||||
# use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
|
||||
# flow_rates=[None] * 32,
|
||||
# offsets=[Coordinate(0, 0, 0)] * 32,
|
||||
# liquid_height=[None] * 32,
|
||||
# blow_out_air_volume=[None] * 32,
|
||||
# spread="wide",
|
||||
# ))
|
||||
asyncio.run(handler.add_liquid(
|
||||
asp_vols=[10]*7,
|
||||
dis_vols=[10]*7,
|
||||
reagent_sources=plate11.children[:7],
|
||||
targets=plate1.children[2:9],
|
||||
use_channels=[0],
|
||||
flow_rates=[None] * 7,
|
||||
offsets=[Coordinate(0, 0, 0)] * 7,
|
||||
liquid_height=[None] * 7,
|
||||
blow_out_air_volume=[None] * 2,
|
||||
delays=None,
|
||||
mix_time=3,
|
||||
mix_vol=5,
|
||||
spread="custom",
|
||||
))
|
||||
|
||||
acid = [20]*8+[40]*8+[60]*8+[80]*8+[100]*8+[120]*8+[140]*8+[160]*8+[180]*8+[200]*8+[220]*8+[240]*8
|
||||
alkaline = acid[::-1] # Reverse the acid list for alkaline
|
||||
asyncio.run(handler.transfer_liquid(
|
||||
asp_vols=acid,
|
||||
dis_vols=acid,
|
||||
tip_racks=[plate1],
|
||||
sources=plate2.children[:],
|
||||
targets=plate5.children[:],
|
||||
use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
|
||||
offsets=[Coordinate(0, 0, 0)] * 32,
|
||||
asp_flow_rates=[None] * 16,
|
||||
dis_flow_rates=[None] * 16,
|
||||
liquid_height=[None] * 32,
|
||||
blow_out_air_volume=[None] * 32,
|
||||
mix_times=3,
|
||||
mix_vol=50,
|
||||
spread="wide",
|
||||
))
|
||||
asyncio.run(handler.run_protocol()) # Run the protocol
|
||||
# # input("Running protocol...")
|
||||
# # input("Press Enter to continue...") # Wait for user input before proceeding
|
||||
# # print("PRCXI9300Handler initialized with deck and host settings.")
|
||||
|
||||
# Example usage
|
||||
# 1. 用导出的json，给每个T1 T2板子设定相应的物料，如果是孔板和枪头盒，要对应区分
|
||||
# 2. 设计一个单点动作流程，可以跑
|
||||
# 3.
|
||||
|
||||
|
||||
# deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
|
||||
|
||||
# from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul,opentrons_96_tiprack_10ul
|
||||
# from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
|
||||
|
||||
# def get_well_container(name: str) -> PRCXI9300Container:
|
||||
# well_containers = corning_96_wellplate_360ul_flat(name).serialize()
|
||||
# plate = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="plate",
|
||||
# ordering=collections.OrderedDict())
|
||||
# plate_serialized = plate.serialize()
|
||||
# plate_serialized["parent_name"] = deck.name
|
||||
# well_containers.update({k: v for k, v in plate_serialized.items() if k not in ["children"]})
|
||||
# new_plate: PRCXI9300Container = PRCXI9300Container.deserialize(well_containers)
|
||||
# return new_plate
|
||||
|
||||
# def get_tip_rack(name: str) -> PRCXI9300Container:
|
||||
# tip_racks = opentrons_96_tiprack_10ul("name").serialize()
|
||||
# tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
|
||||
# ordering=collections.OrderedDict())
|
||||
# tip_rack_serialized = tip_rack.serialize()
|
||||
# tip_rack_serialized["parent_name"] = deck.name
|
||||
# tip_racks.update({k: v for k, v in tip_rack_serialized.items() if k not in ["children"]})
|
||||
# new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
|
||||
# return new_tip_rack
|
||||
|
||||
# plate1 = get_well_container("HPLCPlateT1")
|
||||
# plate1.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "548bbc3df0d4447586f2c19d2c0c0c55",
|
||||
# "Code": "HPLC01",
|
||||
# "Name": "HPLC料盘"
|
||||
# }
|
||||
# })
|
||||
# plate2 = get_well_container("PlateT2")
|
||||
# plate2.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650",
|
||||
# }
|
||||
# })
|
||||
# plate3 = get_well_container("PlateT3")
|
||||
# plate3.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650",
|
||||
# }
|
||||
# })
|
||||
# trash = PRCXI9300Trash(name="trash", size_x=50, size_y=50, size_z=10, category="trash")
|
||||
# trash.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "730067cf07ae43849ddf4034299030e9"
|
||||
# }
|
||||
# })
|
||||
# plate5 = get_well_container("PlateT5")
|
||||
# plate5.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650",
|
||||
# }
|
||||
# })
|
||||
# plate6 = get_well_container("PlateT6")
|
||||
# plate6.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650"
|
||||
# }
|
||||
# })
|
||||
# plate7 = PRCXI9300Container(name="plateT7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
|
||||
# plate7.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650"
|
||||
# }
|
||||
# })
|
||||
# plate8 = get_tip_rack("RackT8")
|
||||
# plate8.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "068b3815e36b4a72a59bae017011b29f",
|
||||
# "Code": "ZX-001-10+",
|
||||
# "Name": "10μL加长 Tip头"
|
||||
# }
|
||||
# })
|
||||
# plate9 = get_well_container("PlateT9")
|
||||
# plate9.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650"
|
||||
# }
|
||||
# })
|
||||
# plate10 = get_well_container("PlateT10")
|
||||
# plate10.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650"
|
||||
# }
|
||||
# })
|
||||
# plate11 = get_well_container("PlateT11")
|
||||
# plate11.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
|
||||
# }
|
||||
# })
|
||||
# plate12 = get_well_container("PlateT12")
|
||||
# plate12.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650"
|
||||
# }
|
||||
# })
|
||||
# plate13 = get_well_container("PlateT13")
|
||||
# plate13.load_state({
|
||||
# "Material": {
|
||||
# "uuid": "04211a2dc93547fe9bf6121eac533650"
|
||||
# }
|
||||
# })
|
||||
|
||||
# # container_for_nothing = PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
|
||||
|
||||
# deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing2", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(trash, location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing3", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing4", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(plate8, location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing5", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing6", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(plate11, location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing8", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
|
||||
|
||||
# handler = PRCXI9300Handler(deck=deck, host="10.181.102.13", port=9999,
|
||||
# timeout=10.0, setup=False, debug=False,
|
||||
# matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1",
|
||||
# channel_num=1, axis="Right") # Initialize the handler with the deck and host settings
|
||||
|
||||
# handler.set_tiprack([plate8]) # Set the tip rack for the handler
|
||||
# asyncio.run(handler.setup()) # Initialize the handler and setup the connection
|
||||
# from pylabrobot.resources import set_volume_tracking
|
||||
# # from pylabrobot.resources import set_tip_tracking
|
||||
# set_volume_tracking(enabled=True)
|
||||
|
||||
# plate11.set_well_liquids([("Water", 100) if (i % 8 == 0 and i // 8 < 6) else (None, 100) for i in range(96)]) # Set liquids for every 8 wells in plate8
|
||||
|
||||
# from unilabos.resources.graphio import *
|
||||
|
||||
# A = tree_to_list([resource_plr_to_ulab(deck)])
|
||||
# # with open("deck.json", "w", encoding="utf-8") as f:
|
||||
# # json.dump(A, f, indent=4, ensure_ascii=False)
|
||||
|
||||
# print(plate11.get_well(0).tracker.get_used_volume())
|
||||
# asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
|
||||
|
||||
# # asyncio.run(handler.pick_up_tips([plate8.children[8]],[0]))
|
||||
# # print(plate8.children[8])
|
||||
# # # asyncio.run(handler.run_protocol())
|
||||
# # asyncio.run(handler.aspirate([plate11.children[0]],[10], [0]))
|
||||
# # print(plate11.children[0])
|
||||
# # # asyncio.run(handler.run_protocol())
|
||||
# # asyncio.run(handler.dispense([plate1.children[0]],[10],[0]))
|
||||
# # print(plate1.children[0])
|
||||
# # # asyncio.run(handler.run_protocol())
|
||||
# # asyncio.run(handler.mix([plate1.children[0]], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
|
||||
# # print(plate1.children[0])
|
||||
# # asyncio.run(handler.discard_tips())
|
||||
|
||||
# asyncio.run(handler.add_liquid(
|
||||
# asp_vols=[10]*7,
|
||||
# dis_vols=[10]*7,
|
||||
# reagent_sources=[plate11.children[0]],
|
||||
# targets=plate1.children[2:9],
|
||||
# use_channels=[0],
|
||||
# flow_rates=[None] * 4,
|
||||
# offsets=[Coordinate(0, 0, 0)] * 4,
|
||||
# liquid_height=[None] * 2,
|
||||
# blow_out_air_volume=[None] * 2,
|
||||
# delays=None,
|
||||
# mix_time=3,
|
||||
# mix_vol=5,
|
||||
# spread="wide",
|
||||
# ))
|
||||
|
||||
# # asyncio.run(handler.transfer_liquid(
|
||||
# # asp_vols=[10]*2,
|
||||
# # dis_vols=[10]*2,
|
||||
|
||||
Reference in New Issue
Block a user