update

unilabos/devices/liquid_handling/liquid_handler_abstract.py

@@ -716,14 +716,14 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
                     raise ValueError(f"Length of `asp_vols` {len(asp_vols)} must match `targets` {len(targets)}.")
                 # 首先应该对任务分组，然后每次1个/8个进行操作处理
                 if len(use_channels) == 1:
-                    tip = []
-                    for x in range(len(use_channels)):
-                        tip.extend(next(self.current_tip))
-                    await self.pick_up_tips(tip)
-
                     for _ in range(len(targets)):
+                        tip = []
+                        for x in range(len(use_channels)):
+                            tip.extend(next(self.current_tip))
+                        await self.pick_up_tips(tip)
+
                         await self.aspirate(
-                            resources=reagent_sources,
+                            resources=[reagent_sources[_]],
                             vols=[asp_vols[_]],
                             use_channels=use_channels,
                             flow_rates=[flow_rates[0]] if flow_rates else None,
@@ -759,17 +759,18 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
                         if delays is not None:
                             await self.custom_delay(seconds=delays[1])
                         await self.touch_tip(targets[_])
-                    await self.discard_tips()
+                        await self.discard_tips()
 
                 elif len(use_channels) == 8:
                     # 对于8个的情况，需要判断此时任务是不是能被8通道移液站来成功处理
                     if len(targets) % 8 != 0:
                         raise ValueError(f"Length of `targets` {len(targets)} must be a multiple of 8 for 8-channel mode.")
-                    tip = []
+
-                    for _ in range(len(use_channels)):
-                        tip.extend(next(self.current_tip))
-                    await self.pick_up_tips(tip)
                     for i in range(0, len(targets), 8):
+                        tip = []
+                        for _ in range(len(use_channels)):
+                            tip.extend(next(self.current_tip))
+                        await self.pick_up_tips(tip)
                         current_targets = targets[i:i + 8]
                         current_reagent_sources = reagent_sources[i:i + 8]
                         current_asp_vols = asp_vols[i:i + 8]
@@ -819,7 +820,7 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
                         if delays is not None:
                             await self.custom_delay(seconds=delays[1])
                         await self.touch_tip(current_targets)
-                    await self.discard_tips()
+                        await self.discard_tips()
 
 
         # except Exception as e:

unilabos/devices/liquid_handling/prcxi/prcxi.py

@@ -977,9 +977,186 @@ if __name__ == "__main__":
     # 4.
 
     
-    deck = PRCXI9300Deck(name="PRCXI_Deck_9300", size_x=100, size_y=100, size_z=100)
+    # deck = PRCXI9300Deck(name="PRCXI_Deck_9300", size_x=100, size_y=100, size_z=100)
 
-    from pylabrobot.resources.opentrons.tip_racks import opentrons_96_tiprack_300ul,opentrons_96_tiprack_10ul
+    # from pylabrobot.resources.opentrons.tip_racks import opentrons_96_tiprack_300ul,opentrons_96_tiprack_10ul
+    # from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
+
+    # def get_well_container(name: str) -> PRCXI9300Container:
+    #     well_containers = corning_96_wellplate_360ul_flat(name).serialize()
+    #     plate = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="plate",
+    #                        ordering=collections.OrderedDict())
+    #     plate_serialized = plate.serialize()
+    #     plate_serialized["parent_name"] = deck.name
+    #     well_containers.update({k: v for k, v in plate_serialized.items() if k not in ["children"]})
+    #     new_plate: PRCXI9300Container = PRCXI9300Container.deserialize(well_containers)
+    #     return new_plate
+
+    # def get_tip_rack(name: str) -> PRCXI9300Container:
+    #     tip_racks = opentrons_96_tiprack_300ul("name").serialize()
+    #     tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
+    #                        ordering=collections.OrderedDict())
+    #     tip_rack_serialized = tip_rack.serialize()
+    #     tip_rack_serialized["parent_name"] = deck.name
+    #     tip_racks.update({k: v for k, v in tip_rack_serialized.items() if k not in ["children"]})
+    #     new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
+    #     return new_tip_rack
+    
+    # plate1 = get_tip_rack("RackT1")
+    # plate1.load_state({
+    #     "Material": {
+    #         "uuid": "076250742950465b9d6ea29a225dfb00",
+    #         "Code": "ZX-001-300",
+    #         "Name": "300μL Tip头"
+    #     }
+    # })
+
+    # plate2 = get_well_container("PlateT2")
+    # plate2.load_state({
+    #     "Material": {
+    #         "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+    #         "Code": "ZX-019-2.2",
+    #         "Name": "96深孔板"
+    #     }
+    # })
+
+
+    # plate3 = PRCXI9300Trash("trash", size_x=50, size_y=100, size_z=10, category="trash")
+    # plate3.load_state({
+    #     "Material": {
+    #         "uuid": "730067cf07ae43849ddf4034299030e9"
+    #     }
+    # })
+
+    # plate4 = get_well_container("PlateT4")
+    # plate4.load_state({
+    #     "Material": {
+    #         "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+    #         "Code": "ZX-019-2.2",
+    #         "Name": "96深孔板"
+    #     }
+    # })
+
+    # plate5 = get_well_container("PlateT5")
+    # plate5.load_state({
+    #     "Material": {
+    #         "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+    #         "Code": "ZX-019-2.2",
+    #         "Name": "96深孔板"
+    #     }
+    # })
+    # plate6 = get_well_container("PlateT6")
+
+    # plate6.load_state({
+    #     "Material": {
+    #         "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+    #         "Code": "ZX-019-2.2",
+    #         "Name": "96深孔板"
+    #     }
+    # })
+
+    # deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate2, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate3, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate4, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate5, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate6, location=Coordinate(0, 0, 0))
+
+    # # print(plate2)
+    # plate_2_liquids = [[('water', 500)]]*96
+    # plate2.set_well_liquids(plate_2_liquids)
+
+    # handler = PRCXI9300Handler(deck=deck, host="10.181.214.132", port=9999, 
+    #                            timeout=10.0, setup=False, debug=False, 
+    #                            matrix_id="71593",
+    #                            channel_num=8, axis="Left")  # Initialize the handler with the deck and host settings
+    
+
+    # handler.set_tiprack([plate1]) 
+
+    # asyncio.run(handler.setup())  # Initialize the handler and setup the connection
+    # from pylabrobot.resources import set_volume_tracking
+    # # from pylabrobot.resources import set_tip_tracking
+    # set_volume_tracking(enabled=True)
+    # from unilabos.resources.graphio import *
+    # A = tree_to_list([resource_plr_to_ulab(deck)])
+    # with open("deck_9300_new.json", "w", encoding="utf-8") as f:
+    #     json.dump(A, f, indent=4, ensure_ascii=False)
+    # asyncio.run(handler.create_protocol(protocol_name="Test Protocol"))  # Initialize the backend and setup the connection
+
+    # # asyncio.run(handler.pick_up_tips(plate1.children[:8],[0,1,2,3,4,5,6,7]))
+    # # print(plate1.children[:8])
+    # # asyncio.run(handler.aspirate(plate2.children[:8],[50]*8, [0,1,2,3,4,5,6,7]))
+    # # print(plate2.children[:8])
+    # # asyncio.run(handler.dispense(plate5.children[:8],[50]*8,[0,1,2,3,4,5,6,7]))
+    # # print(plate5.children[:8])
+
+    # # # # # asyncio.run(handler.drop_tips(tip_rack.children[8:16],[0,1,2,3,4,5,6,7]))
+    # # asyncio.run(handler.discard_tips())
+
+    # # asyncio.run(handler.mix(well_containers.children[:8
+    # # ], mix_time=3, mix_vol=50, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
+    # # #print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2))  # Print matrix info
+    # # asyncio.run(handler.add_liquid(
+    # #     asp_vols=[100]*16,
+    # #     dis_vols=[100]*16,
+    # #     reagent_sources=plate2.children[:16],
+    # #     targets=plate5.children[:16],
+    # #     use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
+    # #     flow_rates=[None] * 32,
+    # #     offsets=[Coordinate(0, 0, 0)] * 32,
+    # #     liquid_height=[None] * 16,
+    # #     blow_out_air_volume=[None] * 16,
+    # #     delays=None,
+    # #     mix_time=3,
+    # #     mix_vol=50,
+    # #     spread="wide",
+    # # ))
+    # # asyncio.run(handler.run_protocol())  # Run the protocol
+    # # asyncio.run(handler.remove_liquid(
+    # #     vols=[100]*16,
+    # #     sources=plate2.children[-16:],
+    # #     waste_liquid=plate5.children[:16], # 这个有些奇怪，但是好像也只能这么写
+    # #     use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
+    # #     flow_rates=[None] * 32,
+    # #     offsets=[Coordinate(0, 0, 0)] * 32,
+    # #     liquid_height=[None] * 32,
+    # #     blow_out_air_volume=[None] * 32,
+    # #     spread="wide",
+    # # ))
+
+    # acid = [20]*8+[40]*8+[60]*8+[80]*8+[100]*8+[120]*8+[140]*8+[160]*8+[180]*8+[200]*8+[220]*8+[240]*8
+    # alkaline = acid[::-1]  # Reverse the acid list for alkaline
+    # asyncio.run(handler.transfer_liquid(
+    #     asp_vols=acid,
+    #     dis_vols=acid,
+    #     tip_racks=[plate1],
+    #     sources=plate2.children[:],
+    #     targets=plate5.children[:],
+    #     use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
+    #     offsets=[Coordinate(0, 0, 0)] * 32,
+    #     asp_flow_rates=[None] * 16,
+    #     dis_flow_rates=[None] * 16,
+    #     liquid_height=[None] * 32,
+    #     blow_out_air_volume=[None] * 32,
+    #     mix_times=3,
+    #     mix_vol=50,
+    #     spread="wide",
+    # ))
+    # asyncio.run(handler.run_protocol())  # Run the protocol
+    # # # input("Running protocol...")
+    # # # input("Press Enter to continue...")  # Wait for user input before proceeding
+    # # # print("PRCXI9300Handler initialized with deck and host settings.")
+
+    # # Example usage
+    # # 1. 用导出的json，给每个T1 T2板子设定相应的物料，如果是孔板和枪头盒，要对应区分
+    # # 2. 设计一个单点动作流程，可以跑
+    # # 3.
+
+
+    deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
+
+    from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul,opentrons_96_tiprack_10ul
     from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
 
     def get_well_container(name: str) -> PRCXI9300Container:
@@ -993,7 +1170,7 @@ if __name__ == "__main__":
         return new_plate
 
     def get_tip_rack(name: str) -> PRCXI9300Container:
-        tip_racks = opentrons_96_tiprack_300ul("name").serialize()
+        tip_racks = opentrons_96_tiprack_10ul("name").serialize()
         tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
                            ordering=collections.OrderedDict())
         tip_rack_serialized = tip_rack.serialize()
@@ -1002,333 +1179,157 @@ if __name__ == "__main__":
         new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
         return new_tip_rack
     
-    plate1 = get_tip_rack("RackT1")
+    plate1 = get_well_container("HPLCPlateT1")
     plate1.load_state({
         "Material": {
-            "uuid": "076250742950465b9d6ea29a225dfb00",
+            "uuid": "548bbc3df0d4447586f2c19d2c0c0c55",
-            "Code": "ZX-001-300",
+            "Code": "HPLC01",
-            "Name": "300μL Tip头"
+            "Name":  "HPLC料盘"
         }
     })
-
     plate2 = get_well_container("PlateT2")
     plate2.load_state({
         "Material": {
-            "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+            "uuid": "04211a2dc93547fe9bf6121eac533650",
-            "Code": "ZX-019-2.2",
-            "Name": "96深孔板"
         }
     })
-
+    plate3 = get_well_container("PlateT3")
-
-    plate3 = PRCXI9300Trash("trash", size_x=50, size_y=100, size_z=10, category="trash")
     plate3.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650",
+        }
+    })
+    trash = PRCXI9300Trash(name="trash", size_x=50, size_y=50, size_z=10, category="trash")
+    trash.load_state({
         "Material": {
             "uuid": "730067cf07ae43849ddf4034299030e9"
         }
     })
-
-    plate4 = get_well_container("PlateT4")
-    plate4.load_state({
-        "Material": {
-            "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
-            "Code": "ZX-019-2.2",
-            "Name": "96深孔板"
-        }
-    })
-
     plate5 = get_well_container("PlateT5")
     plate5.load_state({
         "Material": {
-            "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+            "uuid": "04211a2dc93547fe9bf6121eac533650",
-            "Code": "ZX-019-2.2",
-            "Name": "96深孔板"
         }
     })
     plate6 = get_well_container("PlateT6")
-
     plate6.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+    plate7 = PRCXI9300Container(name="plateT7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate7.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+    plate8 = get_tip_rack("RackT8")
+    plate8.load_state({
+        "Material": {
+            "uuid": "068b3815e36b4a72a59bae017011b29f",
+            "Code": "ZX-001-10+",
+            "Name": "10μL加长 Tip头"
+        }
+    })
+    plate9 = get_well_container("PlateT9")
+    plate9.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+    plate10 = get_well_container("PlateT10")
+    plate10.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+    plate11 = get_well_container("PlateT11")
+    plate11.load_state({
         "Material": {
             "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
-            "Code": "ZX-019-2.2",
+        }
-            "Name": "96深孔板"
+    })
+    plate12 = get_well_container("PlateT12")
+    plate12.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+    plate13 = get_well_container("PlateT13")
+    plate13.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
         }
     })
 
+    # container_for_nothing = PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+
     deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
-    deck.assign_child_resource(plate2, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-    deck.assign_child_resource(plate3, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing2", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-    deck.assign_child_resource(plate4, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(trash, location=Coordinate(0, 0, 0))
-    deck.assign_child_resource(plate5, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing3", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-    deck.assign_child_resource(plate6, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing4", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate8, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing5", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing6", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate11, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing8", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
 
-    # print(plate2)
+    handler = PRCXI9300Handler(deck=deck, host="10.181.102.13", port=9999, 
-    plate_2_liquids = [[('water', 500)]]*96
-    plate2.set_well_liquids(plate_2_liquids)
-
-    handler = PRCXI9300Handler(deck=deck, host="10.181.214.132", port=9999, 
                                timeout=10.0, setup=False, debug=False, 
-                               matrix_id="71593",
+                               matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1",
-                               channel_num=8, axis="Left")  # Initialize the handler with the deck and host settings
+                               channel_num=1, axis="Right")  # Initialize the handler with the deck and host settings
     
-
+    handler.set_tiprack([plate8])  # Set the tip rack for the handler
-    handler.set_tiprack([plate1]) 
-
     asyncio.run(handler.setup())  # Initialize the handler and setup the connection
     from pylabrobot.resources import set_volume_tracking
     # from pylabrobot.resources import set_tip_tracking
     set_volume_tracking(enabled=True)
+
+    plate11.set_well_liquids([("Water", 100) if (i % 8 == 0 and i // 8 < 6) else (None, 100) for i in range(96)])  # Set liquids for every 8 wells in plate8
+
     from unilabos.resources.graphio import *
+
     A = tree_to_list([resource_plr_to_ulab(deck)])
-    with open("deck_9300_new.json", "w", encoding="utf-8") as f:
+    # with open("deck.json", "w", encoding="utf-8") as f:
-        json.dump(A, f, indent=4, ensure_ascii=False)
+    #     json.dump(A, f, indent=4, ensure_ascii=False)
+
+    print(plate11.get_well(0).tracker.get_used_volume())
     asyncio.run(handler.create_protocol(protocol_name="Test Protocol"))  # Initialize the backend and setup the connection
 
-    # asyncio.run(handler.pick_up_tips(plate1.children[:8],[0,1,2,3,4,5,6,7]))
+    # asyncio.run(handler.pick_up_tips([plate8.children[8]],[0]))
-    # print(plate1.children[:8])
+    # print(plate8.children[8])
-    # asyncio.run(handler.aspirate(plate2.children[:8],[50]*8, [0,1,2,3,4,5,6,7]))
+    # # asyncio.run(handler.run_protocol())
-    # print(plate2.children[:8])
+    # asyncio.run(handler.aspirate([plate11.children[0]],[10], [0]))
-    # asyncio.run(handler.dispense(plate5.children[:8],[50]*8,[0,1,2,3,4,5,6,7]))
+    # print(plate11.children[0])
-    # print(plate5.children[:8])
+    # # asyncio.run(handler.run_protocol())
-
+    # asyncio.run(handler.dispense([plate1.children[0]],[10],[0]))
-    # # # # asyncio.run(handler.drop_tips(tip_rack.children[8:16],[0,1,2,3,4,5,6,7]))
+    # print(plate1.children[0])
+    # # asyncio.run(handler.run_protocol())
+    # asyncio.run(handler.mix([plate1.children[0]], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
+    # print(plate1.children[0])
     # asyncio.run(handler.discard_tips())
 
-    # asyncio.run(handler.mix(well_containers.children[:8
+    asyncio.run(handler.add_liquid(
-    # ], mix_time=3, mix_vol=50, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
+    asp_vols=[10]*7,
-    # #print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2))  # Print matrix info
+    dis_vols=[10]*7,
-    # asyncio.run(handler.add_liquid(
+    reagent_sources=plate11.children[:7],
-    #     asp_vols=[100]*16,
+    targets=plate1.children[2:9],
-    #     dis_vols=[100]*16,
+    use_channels=[0],
-    #     reagent_sources=plate2.children[:16],
+    flow_rates=[None] * 7,
-    #     targets=plate5.children[:16],
+    offsets=[Coordinate(0, 0, 0)] * 7,
-    #     use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
+    liquid_height=[None] * 7,
-    #     flow_rates=[None] * 32,
+    blow_out_air_volume=[None] * 2,
-    #     offsets=[Coordinate(0, 0, 0)] * 32,
+    delays=None,
-    #     liquid_height=[None] * 16,
+    mix_time=3,
-    #     blow_out_air_volume=[None] * 16,
+    mix_vol=5,
-    #     delays=None,
+    spread="custom",
-    #     mix_time=3,
+))
-    #     mix_vol=50,
-    #     spread="wide",
-    # ))
-    # asyncio.run(handler.run_protocol())  # Run the protocol
-    # asyncio.run(handler.remove_liquid(
-    #     vols=[100]*16,
-    #     sources=plate2.children[-16:],
-    #     waste_liquid=plate5.children[:16], # 这个有些奇怪，但是好像也只能这么写
-    #     use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
-    #     flow_rates=[None] * 32,
-    #     offsets=[Coordinate(0, 0, 0)] * 32,
-    #     liquid_height=[None] * 32,
-    #     blow_out_air_volume=[None] * 32,
-    #     spread="wide",
-    # ))
 
-    acid = [20]*8+[40]*8+[60]*8+[80]*8+[100]*8+[120]*8+[140]*8+[160]*8+[180]*8+[200]*8+[220]*8+[240]*8
-    alkaline = acid[::-1]  # Reverse the acid list for alkaline
-    asyncio.run(handler.transfer_liquid(
-        asp_vols=acid,
-        dis_vols=acid,
-        tip_racks=[plate1],
-        sources=plate2.children[:],
-        targets=plate5.children[:],
-        use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
-        offsets=[Coordinate(0, 0, 0)] * 32,
-        asp_flow_rates=[None] * 16,
-        dis_flow_rates=[None] * 16,
-        liquid_height=[None] * 32,
-        blow_out_air_volume=[None] * 32,
-        mix_times=3,
-        mix_vol=50,
-        spread="wide",
-    ))
     asyncio.run(handler.run_protocol())  # Run the protocol
-    # # input("Running protocol...")
-    # # input("Press Enter to continue...")  # Wait for user input before proceeding
-    # # print("PRCXI9300Handler initialized with deck and host settings.")
-
-    # Example usage
-    # 1. 用导出的json，给每个T1 T2板子设定相应的物料，如果是孔板和枪头盒，要对应区分
-    # 2. 设计一个单点动作流程，可以跑
-    # 3.
-
-
-#     deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
-
-#     from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul,opentrons_96_tiprack_10ul
-#     from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
-
-#     def get_well_container(name: str) -> PRCXI9300Container:
-#         well_containers = corning_96_wellplate_360ul_flat(name).serialize()
-#         plate = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="plate",
-#                            ordering=collections.OrderedDict())
-#         plate_serialized = plate.serialize()
-#         plate_serialized["parent_name"] = deck.name
-#         well_containers.update({k: v for k, v in plate_serialized.items() if k not in ["children"]})
-#         new_plate: PRCXI9300Container = PRCXI9300Container.deserialize(well_containers)
-#         return new_plate
-
-#     def get_tip_rack(name: str) -> PRCXI9300Container:
-#         tip_racks = opentrons_96_tiprack_10ul("name").serialize()
-#         tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
-#                            ordering=collections.OrderedDict())
-#         tip_rack_serialized = tip_rack.serialize()
-#         tip_rack_serialized["parent_name"] = deck.name
-#         tip_racks.update({k: v for k, v in tip_rack_serialized.items() if k not in ["children"]})
-#         new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
-#         return new_tip_rack
-    
-#     plate1 = get_well_container("HPLCPlateT1")
-#     plate1.load_state({
-#         "Material": {
-#             "uuid": "548bbc3df0d4447586f2c19d2c0c0c55",
-#             "Code": "HPLC01",
-#             "Name":  "HPLC料盘"
-#         }
-#     })
-#     plate2 = get_well_container("PlateT2")
-#     plate2.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650",
-#         }
-#     })
-#     plate3 = get_well_container("PlateT3")
-#     plate3.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650",
-#         }
-#     })
-#     trash = PRCXI9300Trash(name="trash", size_x=50, size_y=50, size_z=10, category="trash")
-#     trash.load_state({
-#         "Material": {
-#             "uuid": "730067cf07ae43849ddf4034299030e9"
-#         }
-#     })
-#     plate5 = get_well_container("PlateT5")
-#     plate5.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650",
-#         }
-#     })
-#     plate6 = get_well_container("PlateT6")
-#     plate6.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650"
-#         }
-#     })
-#     plate7 = PRCXI9300Container(name="plateT7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
-#     plate7.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650"
-#         }
-#     })
-#     plate8 = get_tip_rack("RackT8")
-#     plate8.load_state({
-#         "Material": {
-#             "uuid": "068b3815e36b4a72a59bae017011b29f",
-#             "Code": "ZX-001-10+",
-#             "Name": "10μL加长 Tip头"
-#         }
-#     })
-#     plate9 = get_well_container("PlateT9")
-#     plate9.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650"
-#         }
-#     })
-#     plate10 = get_well_container("PlateT10")
-#     plate10.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650"
-#         }
-#     })
-#     plate11 = get_well_container("PlateT11")
-#     plate11.load_state({
-#         "Material": {
-#             "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
-#         }
-#     })
-#     plate12 = get_well_container("PlateT12")
-#     plate12.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650"
-#         }
-#     })
-#     plate13 = get_well_container("PlateT13")
-#     plate13.load_state({
-#         "Material": {
-#             "uuid": "04211a2dc93547fe9bf6121eac533650"
-#         }
-#     })
-
-#     # container_for_nothing = PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
-
-#     deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing2", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(trash, location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing3", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing4", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(plate8, location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing5", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing6", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(plate11, location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-#     deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing8", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
-
-#     handler = PRCXI9300Handler(deck=deck, host="10.181.102.13", port=9999, 
-#                                timeout=10.0, setup=False, debug=False, 
-#                                matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1",
-#                                channel_num=1, axis="Right")  # Initialize the handler with the deck and host settings
-    
-#     handler.set_tiprack([plate8])  # Set the tip rack for the handler
-#     asyncio.run(handler.setup())  # Initialize the handler and setup the connection
-#     from pylabrobot.resources import set_volume_tracking
-#     # from pylabrobot.resources import set_tip_tracking
-#     set_volume_tracking(enabled=True)
-
-#     plate11.set_well_liquids([("Water", 100) if (i % 8 == 0 and i // 8 < 6) else (None, 100) for i in range(96)])  # Set liquids for every 8 wells in plate8
-
-#     from unilabos.resources.graphio import *
-
-#     A = tree_to_list([resource_plr_to_ulab(deck)])
-#     # with open("deck.json", "w", encoding="utf-8") as f:
-#     #     json.dump(A, f, indent=4, ensure_ascii=False)
-
-#     print(plate11.get_well(0).tracker.get_used_volume())
-#     asyncio.run(handler.create_protocol(protocol_name="Test Protocol"))  # Initialize the backend and setup the connection
-
-#     # asyncio.run(handler.pick_up_tips([plate8.children[8]],[0]))
-#     # print(plate8.children[8])
-#     # # asyncio.run(handler.run_protocol())
-#     # asyncio.run(handler.aspirate([plate11.children[0]],[10], [0]))
-#     # print(plate11.children[0])
-#     # # asyncio.run(handler.run_protocol())
-#     # asyncio.run(handler.dispense([plate1.children[0]],[10],[0]))
-#     # print(plate1.children[0])
-#     # # asyncio.run(handler.run_protocol())
-#     # asyncio.run(handler.mix([plate1.children[0]], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
-#     # print(plate1.children[0])
-#     # asyncio.run(handler.discard_tips())
-
-#     asyncio.run(handler.add_liquid(
-#     asp_vols=[10]*7,
-#     dis_vols=[10]*7,
-#     reagent_sources=[plate11.children[0]],
-#     targets=plate1.children[2:9],
-#     use_channels=[0],
-#     flow_rates=[None] * 4,
-#     offsets=[Coordinate(0, 0, 0)] * 4,
-#     liquid_height=[None] * 2,
-#     blow_out_air_volume=[None] * 2,
-#     delays=None,
-#     mix_time=3,
-#     mix_vol=5,
-#     spread="wide",
-# ))
-
 # #     asyncio.run(handler.transfer_liquid(
 # #     asp_vols=[10]*2,
 # #     dis_vols=[10]*2,