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同步了Biomek.py 现在应可用

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This commit is contained in:
Guangxin Zhang
2025-06-08 16:58:19 +08:00
parent 6577fe12eb
commit 12c17ec26e
2 changed files with 669 additions and 469 deletions
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534
unilabos/devices/liquid_handling/biomek.py
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@@ -16,20 +16,37 @@ from .liquid_handler_abstract import LiquidHandlerAbstract
import json
from typing import Sequence, Optional, List, Union, Literal
class LiquidHandlerBiomek(LiquidHandlerAbstract):
#class LiquidHandlerBiomek(LiquidHandlerAbstract):
class LiquidHandlerBiomek:
"""
Biomek液体处理器的实现类，继承自LiquidHandlerAbstract。
该类用于处理Biomek液体处理器的特定操作。
"""
def __init__(self, backend=None, deck=None, *args, **kwargs):
super().__init__(backend, deck, *args, **kwargs)
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
self._status = "Idle" # 初始状态为 Idle
self._success = False # 初始成功状态为 False
self._status_queue = kwargs.get("status_queue", None) # 状态队列
self.temp_protocol = {}
self.py32_path = "/opt/py32" # Biomek的Python 3.2路径
# 预定义的仪器分类
self.tip_racks = [
"BC230", "BC1025F", "BC50", "TipRack200", "TipRack1000",
"tip", "tips", "Tip", "Tips"
]
self.reservoirs = [
"AgilentReservoir", "nest_12_reservoir_15ml", "nest_1_reservoir_195ml",
"reservoir", "Reservoir", "waste", "Waste"
]
self.plates_96 = [
"BCDeep96Round", "Matrix96_750uL", "NEST 2ml Deep Well Plate", "nest_96_wellplate_100ul_pcr_full_skirt",
"nest_96_wellplate_200ul_flat", "Matrix96", "96", "plate", "Plate"
]
self.aspirate_techniques = {
'MC P300 high':{
'Position': 'P1',
@@ -88,6 +105,29 @@ class LiquidHandlerBiomek(LiquidHandlerAbstract):
'UseExpression': False}
}
def _get_instrument_type(self, class_name: str) -> str:
"""
根据class_name判断仪器类型
Returns:
str: "tip_rack", "reservoir", "plate_96", 或 "unknown"
"""
# 检查是否是枪头架
for tip_name in self.tip_racks:
if tip_name in class_name:
return "tip_rack"
# 检查是否是储液槽
for reservoir_name in self.reservoirs:
if reservoir_name in class_name:
return "reservoir"
# 检查是否是96孔板
for plate_name in self.plates_96:
if plate_name in class_name:
return "plate_96"
return "unknown"
@classmethod
def deserialize(cls, data: dict, allow_marshal: bool = False) -> LiquidHandler:
@@ -137,7 +177,7 @@ class LiquidHandlerBiomek(LiquidHandlerAbstract):
"date": protocol_date,
"type": protocol_type,
},
"labwares": [],
"labwares": {},
"steps": [],
}
return self.temp_protocol
@@ -303,6 +343,104 @@ class LiquidHandlerBiomek(LiquidHandlerAbstract):
return
def instrument_setup_biomek(
self,
id: str,
parent: str,
slot_on_deck: str,
class_name: str,
liquid_type: list[str],
liquid_volume: list[int],
liquid_input_wells: list[str],
):
"""
设置Biomek仪器的参数配置，按照DeckItems格式
根据不同的仪器类型（容器、tip rack等）设置相应的参数结构
位置作为键，配置列表作为值
"""
# 判断仪器类型
instrument_type = self._get_instrument_type(class_name)
config = None # 初始化为None
if instrument_type == "reservoir":
# 储液槽类型配置
config = {
"Properties": {
"Name": id, # 使用id作为名称
"Device": "",
"liquidtype": liquid_type[0] if liquid_type else "Water",
"BarCode": "",
"SenseEveryTime": False
},
"Known": True,
"Class": f"LabwareClasses\\{class_name}",
"DataSets": {"Volume": {}},
"RuntimeDataSets": {"Volume": {}},
"EvalAmounts": (float(liquid_volume[0]),) if liquid_volume else (0,),
"Nominal": False,
"EvalLiquids": (liquid_type[0],) if liquid_type else ("Water",)
}
elif instrument_type == "plate_96":
# 96孔板类型配置
volume_per_well = float(liquid_volume[0]) if liquid_volume else 0
liquid_per_well = liquid_type[0] if liquid_type else "Water"
config = {
"Properties": {
"Name": id, # 使用id作为名称
"Device": "",
"liquidtype": liquid_per_well,
"BarCode": "",
"SenseEveryTime": False
},
"Known": True,
"Class": f"LabwareClasses\\{class_name}",
"DataSets": {"Volume": {}},
"RuntimeDataSets": {"Volume": {}},
"EvalAmounts": tuple([volume_per_well] * 96),
"Nominal": False,
"EvalLiquids": tuple([liquid_per_well] * 96)
}
elif instrument_type == "tip_rack":
# 枪头架类型配置
tip_config = {
"Class": "TipClasses\\T230",
"Contents": [],
"_RT_Contents": [],
"Used": False,
"RT_Used": False,
"Dirty": False,
"RT_Dirty": False,
"MaxVolumeUsed": 0.0,
"RT_MaxVolumeUsed": 0.0
}
config = {
"Tips": tip_config,
"RT_Tips": tip_config.copy(),
"Properties": {},
"Known": False,
"Class": f"LabwareClasses\\{class_name}",
"DataSets": {"Volume": {}},
"RuntimeDataSets": {"Volume": {}}
}
# 按照DeckItems格式存储：位置作为键，配置列表作为值
if config is not None:
self.temp_protocol["labwares"][slot_on_deck] = [config]
else:
# 空位置
self.temp_protocol["labwares"][slot_on_deck] = []
return
def transfer_biomek(
self,
source: str,
@@ -369,10 +507,12 @@ class LiquidHandlerBiomek(LiquidHandlerAbstract):
transfer_params["items"] = items
transfer_params["Solvent"] = 'Water'
transfer_params["TipLocation"] = tip_rack
self.temp_protocol["steps"].append(transfer_params)
tmp={'transfer': transfer_params}
self.temp_protocol["steps"].append(tmp)
return
def move_biomek(
self,
source: str,
@@ -430,13 +570,18 @@ class LiquidHandlerBiomek(LiquidHandlerAbstract):
if __name__ == "__main__":
print("=== Biomek完整流程测试 ===")
print("包含: 仪器设置 + 完整实验步骤")
# 完整的步骤信息（从biomek.py复制）
steps_info = '''
{
"steps": [
{
"step_number": 1,
"operation": "transfer",
"description": "转移PCR产物或酶促反应液至0.05ml 96孔板中",
"description": "转移PCR产物或酶促反应液至0.5ml 96孔板中",
"parameters": {
"source": "P1",
"target": "P11",
@@ -447,7 +592,7 @@ if __name__ == "__main__":
{
"step_number": 2,
"operation": "transfer",
"description": "加入2倍体积Bind Beads BC至产物中",
"description": "加入2倍体积的Bind Beads BC至产物中",
"parameters": {
"source": "P2",
"target": "P11",
@@ -457,52 +602,34 @@ if __name__ == "__main__":
},
{
"step_number": 3,
"operation": "move_labware",
"description": "移动P11至Orbital1用于振荡混匀",
"parameters": {
"source": "P11",
"target": "Orbital1"
}
},
{
"step_number": 4,
"operation": "oscillation",
"description": "在Orbital1上振荡混匀Bind Beads BC与PCR产物（700-900rpm，300秒）",
"description": "振荡混匀300秒",
"parameters": {
"rpm": 800,
"time": 300
}
},
{
"step_number": 5,
"step_number": 4,
"operation": "move_labware",
"description": "移动混匀后的板回P11",
"parameters": {
"source": "Orbital1",
"target": "P11"
}
},
{
"step_number": 6,
"operation": "move_labware",
"description": "将P11移动到磁力架（P12）吸附3分钟",
"description": "转移至96孔磁力架上吸附3分钟",
"parameters": {
"source": "P11",
"target": "P12"
}
},
{
"step_number": 7,
"step_number": 5,
"operation": "incubation",
"description": "磁力架上室温静置3分钟完成吸附",
"description": "吸附3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 8,
"step_number": 6,
"operation": "transfer",
"description": "去除上清液至废液槽",
"description": "吸弃或倒除上清液",
"parameters": {
"source": "P12",
"target": "P22",
@@ -511,9 +638,9 @@ if __name__ == "__main__":
}
},
{
"step_number": 9,
"step_number": 7,
"operation": "transfer",
"description": "加入300-500μl 75%乙醇清洗",
"description": "加入300-500μl 75%乙醇",
"parameters": {
"source": "P3",
"target": "P12",
@@ -522,34 +649,34 @@ if __name__ == "__main__":
}
},
{
"step_number": 10,
"step_number": 8,
"operation": "move_labware",
"description": "移动清洗板到Orbital1进行振荡",
"description": "移动至振荡器进行振荡混匀",
"parameters": {
"source": "P12",
"target": "Orbital1"
}
},
{
"step_number": 11,
"step_number": 9,
"operation": "oscillation",
"description": "乙醇清洗液振荡混匀（700-900rpm, 45秒）",
"description": "振荡混匀60秒",
"parameters": {
"rpm": 800,
"time": 45
"time": 60
}
},
{
"step_number": 12,
"step_number": 10,
"operation": "move_labware",
"description": "振荡后将板移回磁力架P12吸附",
"description": "转移至96孔磁力架上吸附3分钟",
"parameters": {
"source": "Orbital1",
"target": "P12"
}
},
{
"step_number": 13,
"step_number": 11,
"operation": "incubation",
"description": "吸附3分钟",
"parameters": {
@@ -557,9 +684,9 @@ if __name__ == "__main__":
}
},
{
"step_number": 14,
"step_number": 12,
"operation": "transfer",
"description": "去除乙醇上清液至废液槽",
"description": "吸弃或倒弃废液",
"parameters": {
"source": "P12",
"target": "P22",
@@ -568,9 +695,9 @@ if __name__ == "__main__":
}
},
{
"step_number": 15,
"step_number": 13,
"operation": "transfer",
"description": "第二次加入300-500μl 75%乙醇清洗",
"description": "重复加入75%乙醇",
"parameters": {
"source": "P3",
"target": "P12",
@@ -579,44 +706,44 @@ if __name__ == "__main__":
}
},
{
"step_number": 16,
"step_number": 14,
"operation": "move_labware",
"description": "再次移动清洗板到Orbital1振荡",
"description": "移动至振荡器进行振荡混匀",
"parameters": {
"source": "P12",
"target": "Orbital1"
}
},
{
"step_number": 17,
"step_number": 15,
"operation": "oscillation",
"description": "再次乙醇清洗液振荡混匀（700-900rpm, 45秒）",
"description": "振荡混匀60秒",
"parameters": {
"rpm": 800,
"time": 45
"time": 60
}
},
{
"step_number": 18,
"step_number": 16,
"operation": "move_labware",
"description": "振荡后板送回磁力架P12吸附",
"description": "转移至96孔磁力架上吸附3分钟",
"parameters": {
"source": "Orbital1",
"target": "P12"
}
},
{
"step_number": 19,
"step_number": 17,
"operation": "incubation",
"description": "再次吸附3分钟",
"description": "吸附3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 20,
"step_number": 18,
"operation": "transfer",
"description": "去除乙醇上清液至废液槽",
"description": "吸弃或倒弃废液",
"parameters": {
"source": "P12",
"target": "P22",
@@ -625,7 +752,16 @@ if __name__ == "__main__":
}
},
{
"step_number": 21,
"step_number": 19,
"operation": "move_labware",
"description": "正放96孔板，空气干燥15分钟",
"parameters": {
"source": "P12",
"target": "P13"
}
},
{
"step_number": 20,
"operation": "incubation",
"description": "空气干燥15分钟",
"parameters": {
@@ -633,58 +769,75 @@ if __name__ == "__main__":
}
},
{
"step_number": 22,
"step_number": 21,
"operation": "transfer",
"description": "加30-50μl Elution Buffer洗脱",
"description": "加入30-50μl Elution Buffer",
"parameters": {
"source": "P4",
"target": "P12",
"target": "P13",
"tip_rack": "BC230",
"volume": 40
}
},
{
"step_number": 23,
"step_number": 22,
"operation": "move_labware",
"description": "移动到Orbital1振荡混匀（60秒）",
"description": "移动至振荡器进行振荡混匀",
"parameters": {
"source": "P12",
"source": "P13",
"target": "Orbital1"
}
},
{
"step_number": 24,
"step_number": 23,
"operation": "oscillation",
"description": "Elution Buffer振荡混匀（700-900rpm, 60秒）",
"description": "振荡混匀60秒",
"parameters": {
"rpm": 800,
"time": 60
}
},
{
"step_number": 25,
"step_number": 24,
"operation": "move_labware",
"description": "振荡后送回磁力架P12",
"description": "室温静置3分钟",
"parameters": {
"source": "Orbital1",
"target": "P12"
"target": "P13"
}
},
{
"step_number": 26,
"step_number": 25,
"operation": "incubation",
"description": "室温静置3分钟（洗脱反应）",
"description": "室温静置3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 26,
"operation": "move_labware",
"description": "转移至96孔磁力架上吸附2分钟",
"parameters": {
"source": "P13",
"target": "P12"
}
},
{
"step_number": 27,
"operation": "incubation",
"description": "吸附2分钟",
"parameters": {
"time": 120
}
},
{
"step_number": 28,
"operation": "transfer",
"description": "将上清液（DNA）转移到新板（P13）",
"description": "将DNA转移至新的板中",
"parameters": {
"source": "P12",
"target": "P13",
"target": "P14",
"tip_rack": "BC230",
"volume": 40
}
@@ -692,11 +845,11 @@ if __name__ == "__main__":
]
}
'''
# 完整的labware配置信息
labware_with_liquid = '''
[
{
"id": "Tip Rack BC230 on TL1",
"id": "Tip Rack BC230 TL1",
"parent": "deck",
"slot_on_deck": "TL1",
"class_name": "BC230",
@@ -705,7 +858,7 @@ if __name__ == "__main__":
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on TL2",
"id": "Tip Rack BC230 TL2",
"parent": "deck",
"slot_on_deck": "TL2",
"class_name": "BC230",
@@ -714,7 +867,7 @@ if __name__ == "__main__":
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on TL3",
"id": "Tip Rack BC230 TL3",
"parent": "deck",
"slot_on_deck": "TL3",
"class_name": "BC230",
@@ -723,7 +876,7 @@ if __name__ == "__main__":
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on TL4",
"id": "Tip Rack BC230 TL4",
"parent": "deck",
"slot_on_deck": "TL4",
"class_name": "BC230",
@@ -732,7 +885,7 @@ if __name__ == "__main__":
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on TL5",
"id": "Tip Rack BC230 TL5",
"parent": "deck",
"slot_on_deck": "TL5",
"class_name": "BC230",
@@ -741,7 +894,7 @@ if __name__ == "__main__":
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on P5",
"id": "Tip Rack BC230 P5",
"parent": "deck",
"slot_on_deck": "P5",
"class_name": "BC230",
@@ -750,7 +903,7 @@ if __name__ == "__main__":
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on P6",
"id": "Tip Rack BC230 P6",
"parent": "deck",
"slot_on_deck": "P6",
"class_name": "BC230",
@@ -759,7 +912,7 @@ if __name__ == "__main__":
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on P15",
"id": "Tip Rack BC230 P15",
"parent": "deck",
"slot_on_deck": "P15",
"class_name": "BC230",
@@ -768,7 +921,7 @@ if __name__ == "__main__":
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on P16",
"id": "Tip Rack BC230 P16",
"parent": "deck",
"slot_on_deck": "P16",
"class_name": "BC230",
@@ -780,53 +933,82 @@ if __name__ == "__main__":
"id": "stock plate on P1",
"parent": "deck",
"slot_on_deck": "P1",
"class_name": "nest_12_reservoir_15ml",
"liquid_type": [
"master_mix"
],
"liquid_volume": [10000],
"liquid_input_wells": [
"A1"
]
"class_name": "AgilentReservoir",
"liquid_type": ["PCR product"],
"liquid_volume": [5000],
"liquid_input_wells": ["A1"]
},
{
"id": "stock plate on P2",
"parent": "deck",
"slot_on_deck": "P2",
"class_name": "nest_12_reservoir_15ml",
"liquid_type": [
"bind beads"
],
"liquid_volume": [10000],
"liquid_input_wells": [
"A1"
]
"class_name": "AgilentReservoir",
"liquid_type": ["bind beads"],
"liquid_volume": [100000],
"liquid_input_wells": ["A1"]
},
{
"id": "stock plate on P3",
"parent": "deck",
"slot_on_deck": "P3",
"class_name": "nest_12_reservoir_15ml",
"liquid_type": [
"ethyl alcohol"
],
"liquid_volume": [10000],
"liquid_input_wells": [
"A1"
]
"class_name": "AgilentReservoir",
"liquid_type": ["75% ethanol"],
"liquid_volume": [100000],
"liquid_input_wells": ["A1"]
},
{
"id": "elution buffer on P4",
"id": "stock plate on P4",
"parent": "deck",
"slot_on_deck": "P4",
"class_name": "nest_12_reservoir_15ml",
"liquid_type": [
"elution buffer"
],
"class_name": "AgilentReservoir",
"liquid_type": ["Elution Buffer"],
"liquid_volume": [5000],
"liquid_input_wells": [
"A1"
]
"liquid_input_wells": ["A1"]
},
{
"id": "working plate on P11",
"parent": "deck",
"slot_on_deck": "P11",
"class_name": "BCDeep96Round",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P12",
"parent": "deck",
"slot_on_deck": "P12",
"class_name": "BCDeep96Round",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P13",
"parent": "deck",
"slot_on_deck": "P13",
"class_name": "BCDeep96Round",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P14",
"parent": "deck",
"slot_on_deck": "P14",
"class_name": "BCDeep96Round",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "waste on P22",
"parent": "deck",
"slot_on_deck": "P22",
"class_name": "AgilentReservoir",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "oscillation",
@@ -836,77 +1018,95 @@ if __name__ == "__main__":
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P11",
"parent": "deck",
"slot_on_deck": "P11",
"class_name": "NEST 2ml Deep Well Plate",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "magnetics module on P12",
"parent": "deck",
"slot_on_deck": "P12",
"class_name": "magnetics module",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P13",
"parent": "deck",
"slot_on_deck": "P13",
"class_name": "NEST 2ml Deep Well Plate",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "waste on P22",
"parent": "deck",
"slot_on_deck": "P22",
"class_name": "nest_1_reservoir_195ml",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
}
]
'''
# 创建Biomek液体处理器实例
# 创建handler实例
handler = LiquidHandlerBiomek()
handler.temp_protocol = {
"meta": {},
"labwares": [],
"steps": []
}
# 创建协议
protocol = handler.create_protocol(
protocol_name="DNA纯化完整流程",
protocol_description="使用磁珠进行DNA纯化的完整自动化流程",
protocol_version="1.0",
protocol_author="Biomek系统",
protocol_date="2024-01-01",
protocol_type="DNA_purification"
)
input_steps = json.loads(steps_info)
print("\n=== 第一步：设置所有仪器 ===")
# 解析labware配置
labwares = json.loads(labware_with_liquid)
# 设置所有仪器
instrument_count = 0
for labware in labwares:
print(f"设置仪器: {labware['id']} ({labware['class_name']}) 在位置 {labware['slot_on_deck']}")
handler.instrument_setup_biomek(
id=labware['id'],
parent=labware['parent'],
slot_on_deck=labware['slot_on_deck'],
class_name=labware['class_name'],
liquid_type=labware['liquid_type'],
liquid_volume=labware['liquid_volume'],
liquid_input_wells=labware['liquid_input_wells']
)
instrument_count += 1
print(f"总共设置了 {instrument_count} 个仪器位置")
print("\n=== 第二步：执行实验步骤 ===")
# 解析步骤信息
input_steps = json.loads(steps_info)
# 执行所有步骤
step_count = 0
for step in input_steps['steps']:
operation = step['operation']
parameters = step['parameters']
description = step['description']
print(f"步骤 {step['step_number']}: {description}")
if operation == 'transfer':
handler.transfer_biomek(source=parameters['source'],
handler.transfer_biomek(
source=parameters['source'],
target=parameters['target'],
volume=parameters['volume'],
tip_rack=parameters['tip_rack'],
aspirate_techniques='MC P300 high',
dispense_techniques='MC P300 high')
dispense_techniques='MC P300 high'
)
elif operation == 'move_labware':
handler.move_biomek(source=parameters['source'],
target=parameters['target'])
handler.move_biomek(
source=parameters['source'],
target=parameters['target']
)
elif operation == 'oscillation':
handler.oscillation_biomek(rpm=parameters['rpm'],
time=parameters['time'])
handler.oscillation_biomek(
rpm=parameters['rpm'],
time=parameters['time']
)
elif operation == 'incubation':
handler.incubation_biomek(time=parameters['time'])
handler.incubation_biomek(
time=parameters['time']
)
print(json.dumps(handler.temp_protocol, indent=4))
step_count += 1
print(f"总共执行了 {step_count} 个步骤")
print("\n=== 第三步：保存完整协议 ===")
# 获取脚本目录
script_dir = pathlib.Path(__file__).parent
# 保存完整协议
complete_output_path = script_dir / "complete_biomek_protocol_0608.json"
with open(complete_output_path, 'w', encoding='utf-8') as f:
json.dump(handler.temp_protocol, f, indent=4, ensure_ascii=False)
print(f"完整协议已保存到: {complete_output_path}")
print("\n=== 测试完成 ===")
print("完整的DNA纯化流程已成功转换为Biomek格式！")