diff --git a/unilabos/devices/liquid_handling/biomek.py b/unilabos/devices/liquid_handling/biomek.py
index 35c5f6f7..9bd702d1 100644
--- a/unilabos/devices/liquid_handling/biomek.py
+++ b/unilabos/devices/liquid_handling/biomek.py
@@ -290,20 +290,29 @@ class LiquidHandlerBiomek(LiquidHandlerAbstract):
         处理Biomek的液体转移操作。
 
         """
-        
+        items = []
+
         asp_params = self.aspirate_techniques.get(aspirate_techniques, {})
         dis_params = self.dispense_techniques.get(dispense_techniques, {})
 
+        asp_params['Position'] = source
+        dis_params['Position'] = target
+        asp_params['Volume'] = str(volume)
+        dis_params['Volume'] = str(volume)
+
+        items.append(asp_params)
+        items.append(dis_params)
+
         transfer_params = {
             "Span8": False,
             "Pod": "Pod1",
-            "items": {},                      
+            "items": [],                      
             "Wash": False,
             "Dynamic?": True,
             "AutoSelectActiveWashTechnique": False,
             "ActiveWashTechnique": "",
-            "ChangeTipsBetweenDests": False,
-            "ChangeTipsBetweenSources": True,
+            "ChangeTipsBetweenDests": True,
+            "ChangeTipsBetweenSources": False,
             "DefaultCaption": "",             
             "UseExpression": False,
             "LeaveTipsOn": False,
@@ -320,29 +329,22 @@ class LiquidHandlerBiomek(LiquidHandlerAbstract):
             "SplitVolume": False,
             "SplitVolumeCleaning": False,
             "Stop": "Destinations",
-            "TipLocation": "BC1025F",
+            "TipLocation": "BC230",
             "UseCurrentTips": False,    
-            "UseDisposableTips": True,
+            "UseDisposableTips": False,
             "UseFixedTips": False,
             "UseJIT": True,
             "UseMandrelSelection": True,
             "UseProbes": [True, True, True, True, True, True, True, True],
-            "WashCycles": "1",
+            "WashCycles": "4",
             "WashVolume": "110%",
             "Wizard": False
         }
-
-        items: dict = {}
-        items["Source"] = source
-        items["Destination"] = target
-        items["Volume"] = volume 
         transfer_params["items"] = items
-        transfer_params["Solvent"] =  asp_params['Solvent']
+        transfer_params["Solvent"] = 'Water'
         transfer_params["TipLocation"] = tip_rack
-        transfer_params.update(asp_params)
-        transfer_params.update(dis_params)
         self.temp_protocol["steps"].append(transfer_params)
-        
+
         return 
 
 
@@ -612,20 +614,31 @@ if __name__ == "__main__":
     '''
 
 
-    labware_with_liquid = '''
-    [    {
-            "id": "stock plate on P1",
-            "parent": "deck",
-            "slot_on_deck": "P1",
-            "class_name": "nest_12_reservoir_15ml",
-            "liquid_type": [
-                "master_mix"
-            ],
-            "liquid_volume": [10000],
-            "liquid_input_wells": [
-                "A1"
-            ]
-        },
+
+labware_with_liquid = '''
+[    {
+        "id": "stock plate on P1",
+        "parent": "deck",
+        "slot_on_deck": "P1",
+        "class_name": "nest_12_reservoir_15ml",
+        "liquid_type": [
+            "master_mix"
+        ],
+        "liquid_volume": [10000],
+        "liquid_input_wells": [
+            "A1"
+        ]
+    },
+    {
+        "id": "Tip Rack BC230 TL2",
+        "parent": "deck",
+        "slot_on_deck": "TL2",
+        "class_name": "BC230",
+        "liquid_type": [],
+        "liquid_volume": [],
+        "liquid_input_wells": [
+        ]
+    },
         {
             "id": "Tip Rack BC230 TL2",
             "parent": "deck",
@@ -813,30 +826,30 @@ if __name__ == "__main__":
     
     '''
 
-    handler = LiquidHandlerBiomek()
-    handler.temp_protocol = {
-        "meta": {},
-        "labwares": [],
-        "steps": []
-    }
 
-    input_steps = json.loads(steps_info)
-    labwares = json.loads(labware_with_liquid)
 
-    for step in input_steps['steps']:
-        if step['operation'] != 'transfer':
-            continue
-        parameters = step['parameters']
-        tip_rack=parameters['tip_rack']
-        # 找到labwares中与tip_rack匹配的项的id
-        tip_rack_id = [lw['id'] for lw in labwares if lw['class_name'] == tip_rack][0]
+handler = LiquidHandlerBiomek()
+
+handler.temp_protocol = {
+    "meta": {},
+    "labwares": [],
+    "steps": []
+}
+
+input_steps = json.loads(steps_info)
+labwares = json.loads(labware_with_liquid)
+
+for step in input_steps['steps']:
+    if step['operation'] != 'transfer':
+        continue
+    parameters = step['parameters']
+
+    handler.transfer_biomek(source=parameters['source'],
+                            target=parameters['target'],
+                            volume=parameters['volume'],
+                            tip_rack=parameters['tip_rack'],
+                            aspirate_techniques='MC P300 high',
+                            dispense_techniques='MC P300 high'
+                            )
 
-        handler.transfer_biomek(source=parameters['source'],
-                                target=parameters['target'],
-                                volume=parameters['volume'],
-                                tip_rack=tip_rack_id,
-                                aspirate_techniques='MC P300 high',
-                                dispense_techniques='MC P300 high'
-                                )
-    print(json.dumps(handler.temp_protocol['steps'],indent=4, ensure_ascii=False))
 
diff --git a/unilabos/devices/liquid_handling/biomek.txt b/unilabos/devices/liquid_handling/biomek.txt
new file mode 100644
index 00000000..2d830a6d
--- /dev/null
+++ b/unilabos/devices/liquid_handling/biomek.txt
@@ -0,0 +1,642 @@
+
+ 当前方法 Method0530 包含 36 个步骤
+
+ 步骤 0:
+  Bitmap: OStepUI.ocx,START
+  Let: {}
+  Weak: {}
+  Prompt: {}
+
+ 步骤 1:
+  BarcodeInput?: False
+  DeckItems: {'P1': [{'Properties': {'Name': '', 'Device': '', 'liquidtype': 'Water', 'BarCode': '', 'SenseEveryTime': False}, 'Known': True, 'Class': 'LabwareClasses\\Matrix96_750uL', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}, 'EvalAmounts': (500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0), 'Nominal': False, 'EvalLiquids': ('Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water')}], 'P10': [{'Tips': {'Class': 'TipClasses\\T50F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T50F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC50F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'P8': [], 'P9': [{'Tips': {'Class': 'TipClasses\\T50F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T50F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC50F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'P11': [{'Properties': {'Name': '', 'Device': '', 'liquidtype': 'Water', 'BarCode': '', 'SenseEveryTime': False}, 'Known': True, 'Class': 'LabwareClasses\\BCDeep96Round', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}, 'EvalAmounts': (0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0), 'Nominal': False, 'EvalLiquids': ('Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water')}], 'P12': [], 'P13': [{'Properties': {'Name': '', 'Device': '', 'liquidtype': 'Water', 'BarCode': '', 'SenseEveryTime': False}, 'Known': True, 'Class': 'LabwareClasses\\BCDeep96Round', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}, 'EvalAmounts': (0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0, 0.0), 'Nominal': False, 'EvalLiquids': ('Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water')}], 'P14': [], 'P15': [], 'P16': [{'Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC1025F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'P17': [{'Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC1025F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'P18': [{'Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC1025F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'P19': [{'Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC1025F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'P20': [], 'P2': [{'Properties': {'Name': '', 'Device': '', 'liquidtype': 'Water', 'BarCode': '', 'SenseEveryTime': False}, 'Known': True, 'Class': 'LabwareClasses\\Matrix96_750uL', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}, 'EvalAmounts': (500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0), 'Nominal': False, 'EvalLiquids': ('Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water')}], 'P21': [], 'P22': [{'Properties': {'Name': '', 'Device': '', 'liquidtype': 'Water', 'BarCode': '', 'SenseEveryTime': False}, 'Known': True, 'Class': 'LabwareClasses\\AgilentReservoir', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}, 'EvalAmounts': (0.0,), 'Nominal': False, 'EvalLiquids': ('Water',)}], 'P23': [], 'P24': [], 'P25': [], 'P26': [], 'P27': [], 'P28': [{'Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC1025F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'P29': [{'Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC1025F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'P30': [], 'P3': [{'Properties': {'Name': '', 'Device': '', 'liquidtype': 'Water', 'BarCode': '', 'SenseEveryTime': False}, 'Known': True, 'Class': 'LabwareClasses\\AgilentReservoir', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}, 'EvalAmounts': (300000.0,), 'Nominal': False, 'EvalLiquids': ('Water',)}], 'P4': [{'Properties': {'Name': '', 'Device': '', 'liquidtype': 'Water', 'BarCode': '', 'SenseEveryTime': False}, 'Known': True, 'Class': 'LabwareClasses\\Matrix96_750uL', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}, 'EvalAmounts': (500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0, 500.0), 'Nominal': False, 'EvalLiquids': ('Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water', 'Water')}], 'P5': [], 'P6': [], 'P7': [], 'TL1': [{'Tips': {'Class': 'TipClasses\\T230', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T230', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC230', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'TL2': [{'Tips': {'Class': 'TipClasses\\T230', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T230', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC230', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'TL3': [{'Tips': {'Class': 'TipClasses\\T230', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T230', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC230', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'TL4': [{'Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC1025F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'TL5': [{'Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'RT_Tips': {'Class': 'TipClasses\\T1025F', 'Contents': [], '_RT_Contents': [], 'Used': False, 'RT_Used': False, 'Dirty': False, 'RT_Dirty': False, 'MaxVolumeUsed': 0.0, 'RT_MaxVolumeUsed': 0.0}, 'Properties': {}, 'Known': False, 'Class': 'LabwareClasses\\BC1025F', 'DataSets': {'Volume': {}}, 'RuntimeDataSets': {'Volume': {}}}], 'TR1': [], 'WS1': 'Water'}
+  Layout: Multichannel
+  Pause?: True
+  PodSetup: {'LeftHasTips': False, 'LeftTipType': '', 'RightHasTips': False, 'RightTipType': ''}
+  SplitterPosition: 206
+  VerifyPodSetup?: True
+
+ 步骤 2:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P1', 'Height': -2.0, 'Volume': '50', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'Matrix96_750uL', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P11', 'Height': -2.0, 'Volume': '50', 'liquidtype': 'Tip Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 50 µL from P1 to P11
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC230
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 3:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P2', 'Height': -2.0, 'Volume': '100', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'Matrix96_750uL', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P11', 'Height': -2.0, 'Volume': '100', 'liquidtype': 'Tip Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 100 µL from P2 to P11
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC230
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 4:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: P11
+  Target: Orbital1
+  LeaveBottomLabware: False
+
+ 步骤 5:
+  Device: 
+  Parameters: ()
+  Command: 
+
+ 步骤 6:
+  Device: 
+  Parameters: ()
+  Command: 
+
+ 步骤 7:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: Orbital1
+  Target: P11
+  LeaveBottomLabware: False
+
+ 步骤 8:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: P11
+  Target: P12
+  LeaveBottomLabware: False
+
+ 步骤 9:
+  Message: Paused
+  Location: the whole system
+  Time: 180
+  Mode: TimedResource
+
+ 步骤 10:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P12', 'Height': -2.0, 'Volume': '150', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P22', 'Height': -2.0, 'Volume': '150', 'liquidtype': 'Tip Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 150 µL from P12 to P22
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC230
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 11:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P3', 'Height': -2.0, 'Volume': '400', 'liquidtype': 'Well Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P12', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Tip Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 200 µL from P3 to P12
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC1025F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 12:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P3', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Well Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P12', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Tip Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 200 µL from P3 to P12
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC1025F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 13:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: P12
+  Target: Orbital1
+  LeaveBottomLabware: False
+
+ 步骤 14:
+  Device: 
+  Parameters: ()
+  Command: 
+
+ 步骤 15:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: Orbital1
+  Target: P12
+  LeaveBottomLabware: False
+
+ 步骤 16:
+  Message: Paused
+  Location: the whole system
+  Time: 180
+  Mode: TimedResource
+
+ 步骤 17:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P12', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P22', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Tip Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 200 µL from P12 to P22
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC1025F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 18:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P12', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P22', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Tip Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 200 µL from P12 to P22
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC1025F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 19:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P3', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Well Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P12', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Tip Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 200 µL from P3 to P12
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC1025F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 20:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P3', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Well Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P12', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Tip Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 200 µL from P3 to P12
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC1025F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 21:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: P12
+  Target: Orbital1
+  LeaveBottomLabware: False
+
+ 步骤 22:
+  Device: OrbitalShaker0
+  Parameters: ('800', '3', '45', 'CounterClockwise', None)
+  Command: Timed Shake
+
+ 步骤 23:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: Orbital1
+  Target: P12
+  LeaveBottomLabware: False
+
+ 步骤 24:
+  Message: Paused
+  Location: the whole system
+  Time: 180
+  Mode: TimedResource
+
+ 步骤 25:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P12', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P22', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Tip Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 200 µL from P12 to P22
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC1025F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 26:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P12', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P22', 'Height': -2.0, 'Volume': '200', 'liquidtype': 'Tip Contents', 'LabwareClass': 'AgilentReservoir', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True,), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 200 µL from P12 to P22
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC1025F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 27:
+  Message: Paused
+  Location: the whole system
+  Time: 900
+  Mode: TimedResource
+
+ 步骤 28:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P4', 'Height': -2.0, 'Volume': '40', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'Matrix96_750uL', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P12', 'Height': -2.0, 'Volume': '40', 'liquidtype': 'Tip Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 40 µL from P4 to P12
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC50F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 29:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: P12
+  Target: Orbital1
+  LeaveBottomLabware: False
+
+ 步骤 30:
+  Device: OrbitalShaker0
+  Parameters: ('800', '3', '60', 'CounterClockwise', None)
+  Command: Timed Shake
+
+ 步骤 31:
+  Message: Paused
+  Location: the whole system
+  Time: 180
+  Mode: TimedResource
+
+ 步骤 32:
+  Pod: Pod1
+  GripSide: A1 near
+  Source: Orbital1
+  Target: P12
+  LeaveBottomLabware: False
+
+ 步骤 33:
+  Message: Paused
+  Location: the whole system
+  Time: 120
+  Mode: TimedResource
+
+ 步骤 34:
+  Span8: False
+  Pod: Pod1
+  Wash: False
+  items: [{'Position': 'P12', 'Height': -2.0, 'Volume': '40', 'liquidtype': 'Well Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': True, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}, {'Position': 'P13', 'Height': -2.0, 'Volume': '40', 'liquidtype': 'Tip Contents', 'WellsX': 12, 'LabwareClass': 'BCDeep96Round', 'AutoSelectPrototype': True, 'ColsFirst': True, 'CustomHeight': False, 'DataSetPattern': False, 'HeightFrom': 0, 'LocalPattern': True, 'Operation': 'Dispense', 'OverrideHeight': False, 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC', 'ReferencedPattern': '', 'RowsFirst': False, 'SectionExpression': '', 'SelectionInfo': (1,), 'SetMark': True, 'Source': False, 'StartAtMark': False, 'StartAtSelection': True, 'UseExpression': False}]
+  Dynamic?: True
+  AutoSelectActiveWashTechnique: False
+  ActiveWashTechnique: 
+  ChangeTipsBetweenDests: True
+  ChangeTipsBetweenSources: False
+  DefaultCaption: Transfer 40 µL from P12 to P13
+  UseExpression: False
+  LeaveTipsOn: False
+  MandrelExpression: 
+  Repeats: 1
+  RepeatsByVolume: False
+  Replicates: 1
+  ShowTipHandlingDetails: True
+  ShowTransferDetails: True
+  Solvent: Water
+  Span8Wash: False
+  Span8WashVolume: 2
+  Span8WasteVolume: 1
+  SplitVolume: False
+  SplitVolumeCleaning: False
+  Stop: Destinations
+  TipLocation: BC50F
+  UseCurrentTips: False
+  UseDisposableTips: False
+  UseFixedTips: False
+  UseJIT: True
+  UseMandrelSelection: True
+  UseProbes: (True, True, True, True, True, True, True, True)
+  WashCycles: 4
+  WashVolume: 110%
+  Wizard: False
+
+ 步骤 35:
+  Type: 
+  Bitmap: OStepUI.ocx,FINISH
+  cleardeck: True
+  cleardevices: True
+  cleanuppods: True
+  PodsToMaxZ: True
+  ClearGlobals: True
+  ParkPods: True
+  Authent: False
+  Collapsed: True
+  ConnectionString: 
+  Password: 
+  Path: 
+  Report: False
+  Server: 
+  TableName: 
+  UserName: 
+  Catalog: 
diff --git a/unilabos/devices/liquid_handling/biomek_temporary_protocol.json b/unilabos/devices/liquid_handling/biomek_temporary_protocol.json
new file mode 100644
index 00000000..129c3ba2
--- /dev/null
+++ b/unilabos/devices/liquid_handling/biomek_temporary_protocol.json
@@ -0,0 +1,2697 @@
+{
+    "meta": {},
+    "labwares": [],
+    "steps": [
+        {
+            "Span8": false,
+            "Pod": "Pod1",
+            "items": [
+                {
+                    "Position": "P12",
+                    "Height": -2.0,
+                    "Volume": "40",
+                    "liquidtype": "Well Contents",
+                    "WellsX": 12,
+                    "LabwareClass": "Matrix96_750uL",
+                    "AutoSelectPrototype": true,
+                    "ColsFirst": true,
+                    "CustomHeight": false,
+                    "DataSetPattern": false,
+                    "HeightFrom": 0,
+                    "LocalPattern": true,
+                    "Operation": "Aspirate",
+                    "OverrideHeight": false,
+                    "Pattern": [
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
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+                        true,
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+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
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+                        true,
+                        true,
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+                        true,
+                        true,
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+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true
+                    ],
+                    "Prototype": "MC P300 High",
+                    "ReferencedPattern": "",
+                    "RowsFirst": false,
+                    "SectionExpression": "",
+                    "SelectionInfo": [
+                        1
+                    ],
+                    "SetMark": true,
+                    "Source": false,
+                    "StartAtMark": false,
+                    "StartAtSelection": true,
+                    "UseExpression": false
+                }
+            ],
+            "Wash": false,
+            "Dynamic?": true,
+            "AutoSelectActiveWashTechnique": false,
+            "ActiveWashTechnique": "",
+            "ChangeTipsBetweenDests": true,
+            "ChangeTipsBetweenSources": false,
+            "DefaultCaption": "",
+            "UseExpression": false,
+            "LeaveTipsOn": false,
+            "MandrelExpression": "",
+            "Repeats": "1",
+            "RepeatsByVolume": false,
+            "Replicates": "1",
+            "ShowTipHandlingDetails": false,
+            "ShowTransferDetails": true,
+            "Solvent": "Water",
+            "Span8Wash": false,
+            "Span8WashVolume": "2",
+            "Span8WasteVolume": "1",
+            "SplitVolume": false,
+            "SplitVolumeCleaning": false,
+            "Stop": "Destinations",
+            "TipLocation": "BC230",
+            "UseCurrentTips": false,
+            "UseDisposableTips": false,
+            "UseFixedTips": false,
+            "UseJIT": true,
+            "UseMandrelSelection": true,
+            "UseProbes": [
+                true,
+                true,
+                true,
+                true,
+                true,
+                true,
+                true,
+                true
+            ],
+            "WashCycles": "4",
+            "WashVolume": "110%",
+            "Wizard": false
+        },
+        {
+            "Span8": false,
+            "Pod": "Pod1",
+            "items": [
+                {
+                    "Position": "P12",
+                    "Height": -2.0,
+                    "Volume": "40",
+                    "liquidtype": "Well Contents",
+                    "WellsX": 12,
+                    "LabwareClass": "Matrix96_750uL",
+                    "AutoSelectPrototype": true,
+                    "ColsFirst": true,
+                    "CustomHeight": false,
+                    "DataSetPattern": false,
+                    "HeightFrom": 0,
+                    "LocalPattern": true,
+                    "Operation": "Aspirate",
+                    "OverrideHeight": false,
+                    "Pattern": [
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true
+                    ],
+                    "Prototype": "MC P300 High",
+                    "ReferencedPattern": "",
+                    "RowsFirst": false,
+                    "SectionExpression": "",
+                    "SelectionInfo": [
+                        1
+                    ],
+                    "SetMark": true,
+                    "Source": true,
+                    "StartAtMark": false,
+                    "StartAtSelection": true,
+                    "UseExpression": false
+                },
+                {
+                    "Position": "P13",
+                    "Height": -2.0,
+                    "Volume": "40",
+                    "liquidtype": "Tip Contents",
+                    "WellsX": 12,
+                    "LabwareClass": "Matrix96_750uL",
+                    "AutoSelectPrototype": true,
+                    "ColsFirst": true,
+                    "CustomHeight": false,
+                    "DataSetPattern": false,
+                    "HeightFrom": 0,
+                    "LocalPattern": true,
+                    "Operation": "Dispense",
+                    "OverrideHeight": false,
+                    "Pattern": [
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true,
+                        true
+                    ],
+                    "Prototype": "MC P300 High",
+                    "ReferencedPattern": "",
+                    "RowsFirst": false,
+                    "SectionExpression": "",
+                    "SelectionInfo": [
+                        1
+                    ],
+                    "SetMark": true,
+                    "Source": false,
+                    "StartAtMark": false,
+                    "StartAtSelection": true,
+                    "UseExpression": false
+                }
+            ],
+            "Wash": false,
+            "Dynamic?": true,
+            "AutoSelectActiveWashTechnique": false,
+            "ActiveWashTechnique": "",
+            "ChangeTipsBetweenDests": true,
+            "ChangeTipsBetweenSources": false,
+            "DefaultCaption": "",
+            "UseExpression": false,
+            "LeaveTipsOn": false,
+            "MandrelExpression": "",
+            "Repeats": "1",
+            "RepeatsByVolume": false,
+            "Replicates": "1",
+            "ShowTipHandlingDetails": false,
+            "ShowTransferDetails": true,
+            "Solvent": "Water",
+            "Span8Wash": false,
+            "Span8WashVolume": "2",
+            "Span8WasteVolume": "1",
+            "SplitVolume": false,
+            "SplitVolumeCleaning": false,
+            "Stop": "Destinations",
+            "TipLocation": "BC230",
+            "UseCurrentTips": false,
+            "UseDisposableTips": false,
+            "UseFixedTips": false,
+            "UseJIT": true,
+            "UseMandrelSelection": true,
+            "UseProbes": [
+                true,
+                true,
+                true,
+                true,
+                true,
+                true,
+                true,
+                true
+            ],
+            "WashCycles": "4",
+            "WashVolume": "110%",
+            "Wizard": false
+        }
+    ]
+}
\ No newline at end of file
diff --git a/unilabos/devices/liquid_handling/biomek_test.py b/unilabos/devices/liquid_handling/biomek_test.py
index e8f51388..424d5ac7 100644
--- a/unilabos/devices/liquid_handling/biomek_test.py
+++ b/unilabos/devices/liquid_handling/biomek_test.py
@@ -15,269 +15,6 @@ from pylabrobot.resources import (
 import json
 from typing import Sequence, Optional, List, Union, Literal
 
-steps_info = ''' 
-{
-  "steps": [
-    {
-      "step_number": 1,
-      "operation": "transfer",
-      "description": "转移PCR产物或酶促反应液至0.05ml 96孔板中",
-      "parameters": {
-        "source": "P1",
-        "target": "P11",
-        "tip_rack": "BC230",
-        "volume": 50
-      }
-    },
-    {
-      "step_number": 2,
-      "operation": "transfer",
-      "description": "加入2倍体积Bind Beads BC至产物中",
-      "parameters": {
-        "source": "P2",
-        "target": "P11",
-        "tip_rack": "BC230",
-        "volume": 100
-      }
-    },
-    {
-      "step_number": 3,
-      "operation": "move_labware",
-      "description": "移动P11至Orbital1用于振荡混匀",
-      "parameters": {
-        "source": "P11",
-        "target": "Orbital1"
-      }
-    },
-    {
-      "step_number": 4,
-      "operation": "oscillation",
-      "description": "在Orbital1上振荡混匀Bind Beads BC与PCR产物（700-900rpm，300秒）",
-      "parameters": {
-        "rpm": 800,
-        "time": 300
-      }
-    },
-    {
-      "step_number": 5,
-      "operation": "move_labware",
-      "description": "移动混匀后的板回P11",
-      "parameters": {
-        "source": "Orbital1",
-        "target": "P11"
-      }
-    },
-    {
-      "step_number": 6,
-      "operation": "move_labware",
-      "description": "将P11移动到磁力架（P12）吸附3分钟",
-      "parameters": {
-        "source": "P11",
-        "target": "P12"
-      }
-    },
-    {
-      "step_number": 7,
-      "operation": "incubation",
-      "description": "磁力架上室温静置3分钟完成吸附",
-      "parameters": {
-        "time": 180
-      }
-    },
-    {
-      "step_number": 8,
-      "operation": "transfer",
-      "description": "去除上清液至废液槽",
-      "parameters": {
-        "source": "P12",
-        "target": "P22",
-        "tip_rack": "BC230",
-        "volume": 150
-      }
-    },
-    {
-      "step_number": 9,
-      "operation": "transfer",
-      "description": "加入300-500μl 75%乙醇清洗",
-      "parameters": {
-        "source": "P3",
-        "target": "P12",
-        "tip_rack": "BC230",
-        "volume": 400
-      }
-    },
-    {
-      "step_number": 10,
-      "operation": "move_labware",
-      "description": "移动清洗板到Orbital1进行振荡",
-      "parameters": {
-        "source": "P12",
-        "target": "Orbital1"
-      }
-    },
-    {
-      "step_number": 11,
-      "operation": "oscillation",
-      "description": "乙醇清洗液振荡混匀（700-900rpm, 45秒）",
-      "parameters": {
-        "rpm": 800,
-        "time": 45
-      }
-    },
-    {
-      "step_number": 12,
-      "operation": "move_labware",
-      "description": "振荡后将板移回磁力架P12吸附",
-      "parameters": {
-        "source": "Orbital1",
-        "target": "P12"
-      }
-    },
-    {
-      "step_number": 13,
-      "operation": "incubation",
-      "description": "吸附3分钟",
-      "parameters": {
-        "time": 180
-      }
-    },
-    {
-      "step_number": 14,
-      "operation": "transfer",
-      "description": "去除乙醇上清液至废液槽",
-      "parameters": {
-        "source": "P12",
-        "target": "P22",
-        "tip_rack": "BC230",
-        "volume": 400
-      }
-    },
-    {
-      "step_number": 15,
-      "operation": "transfer",
-      "description": "第二次加入300-500μl 75%乙醇清洗",
-      "parameters": {
-        "source": "P3",
-        "target": "P12",
-        "tip_rack": "BC230",
-        "volume": 400
-      }
-    },
-    {
-      "step_number": 16,
-      "operation": "move_labware",
-      "description": "再次移动清洗板到Orbital1振荡",
-      "parameters": {
-        "source": "P12",
-        "target": "Orbital1"
-      }
-    },
-    {
-      "step_number": 17,
-      "operation": "oscillation",
-      "description": "再次乙醇清洗液振荡混匀（700-900rpm, 45秒）",
-      "parameters": {
-        "rpm": 800,
-        "time": 45
-      }
-    },
-    {
-      "step_number": 18,
-      "operation": "move_labware",
-      "description": "振荡后板送回磁力架P12吸附",
-      "parameters": {
-        "source": "Orbital1",
-        "target": "P12"
-      }
-    },
-    {
-      "step_number": 19,
-      "operation": "incubation",
-      "description": "再次吸附3分钟",
-      "parameters": {
-        "time": 180
-      }
-    },
-    {
-      "step_number": 20,
-      "operation": "transfer",
-      "description": "去除乙醇上清液至废液槽",
-      "parameters": {
-        "source": "P12",
-        "target": "P22",
-        "tip_rack": "BC230",
-        "volume": 400
-      }
-    },
-    {
-      "step_number": 21,
-      "operation": "incubation",
-      "description": "空气干燥15分钟",
-      "parameters": {
-        "time": 900
-      }
-    },
-    {
-      "step_number": 22,
-      "operation": "transfer",
-      "description": "加30-50μl Elution Buffer洗脱",
-      "parameters": {
-        "source": "P4",
-        "target": "P12",
-        "tip_rack": "BC230",
-        "volume": 40
-      }
-    },
-    {
-      "step_number": 23,
-      "operation": "move_labware",
-      "description": "移动到Orbital1振荡混匀（60秒）",
-      "parameters": {
-        "source": "P12",
-        "target": "Orbital1"
-      }
-    },
-    {
-      "step_number": 24,
-      "operation": "oscillation",
-      "description": "Elution Buffer振荡混匀（700-900rpm, 60秒）",
-      "parameters": {
-        "rpm": 800,
-        "time": 60
-      }
-    },
-    {
-      "step_number": 25,
-      "operation": "move_labware",
-      "description": "振荡后送回磁力架P12",
-      "parameters": {
-        "source": "Orbital1",
-        "target": "P12"
-      }
-    },
-    {
-      "step_number": 26,
-      "operation": "incubation",
-      "description": "室温静置3分钟（洗脱反应）",
-      "parameters": {
-        "time": 180
-      }
-    },
-    {
-      "step_number": 27,
-      "operation": "transfer",
-      "description": "将上清液（DNA）转移到新板（P13）",
-      "parameters": {
-        "source": "P12",
-        "target": "P13",
-        "tip_rack": "BC230",
-        "volume": 40
-      }
-    }
-  ]
-}
-'''
-
 
 
 #class LiquidHandlerBiomek(LiquidHandlerAbstract):
@@ -298,44 +35,60 @@ class LiquidHandlerBiomek:
         self.py32_path = "/opt/py32"  # Biomek的Python 3.2路径
         self.aspirate_techniques = {
             'MC P300 high':{       
-                "Solvent": "Water",
+                            'Position': 'P1', 
+                            'Height': -2.0, 
+                            'Volume': '50', 
+                            'liquidtype': 'Well Contents', 
+                            'WellsX': 12, 
+                            'LabwareClass': 'Matrix96_750uL', 
+                            'AutoSelectPrototype': True, 
+                            'ColsFirst': True, 
+                            'CustomHeight': False, 
+                            'DataSetPattern': False, 
+                            'HeightFrom': 0, 
+                            'LocalPattern': True, 
+                            'Operation': 'Aspirate', 
+                            'OverrideHeight': False, 
+                            'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True),
+                            'Prototype': 'MC P300 High', 
+                            'ReferencedPattern': '', 
+                            'RowsFirst': False, 
+                            'SectionExpression': '', 
+                            'SelectionInfo': (1,), 
+                            'SetMark': True, 
+                            'Source': True, 
+                            'StartAtMark': False, 
+                            'StartAtSelection': True, 
+                            'UseExpression': False},
             }
-        }
+      
         self.dispense_techniques = {
             'MC P300 high':{       
-            "Span8": False,
-            "Pod": "Pod1",
-            "Wash": False,
-            "Dynamic?": True,
-            "AutoSelectActiveWashTechnique": False,
-            "ActiveWashTechnique": "",
-            "ChangeTipsBetweenDests": True,
-            "ChangeTipsBetweenSources": False,
-            "DefaultCaption": "",             
-            "UseExpression": False,
-            "LeaveTipsOn": False,
-            "MandrelExpression": "",
-            "Repeats": "1",
-            "RepeatsByVolume": False,
-            "Replicates": "1",
-            "ShowTipHandlingDetails": False,
-            "ShowTransferDetails": True,
-            "Span8Wash": False,
-            "Span8WashVolume": "2",
-            "Span8WasteVolume": "1",
-            "SplitVolume": False,
-            "SplitVolumeCleaning": False,
-            "Stop": "Destinations",
-            "UseCurrentTips": False,    
-            "UseDisposableTips": False,
-            "UseFixedTips": False,
-            "UseJIT": True,
-            "UseMandrelSelection": True,
-            "UseProbes": [True, True, True, True, True, True, True, True],
-            "WashCycles": "3",
-            "WashVolume": "110%",
-            "Wizard": False
-            }
+                          'Position': 'P11', 
+                          'Height': -2.0, 
+                          'Volume': '50', 
+                          'liquidtype': 'Tip Contents', 
+                          'WellsX': 12, 
+                          'LabwareClass': 'Matrix96_750uL', 
+                          'AutoSelectPrototype': True, 
+                          'ColsFirst': True, 
+                          'CustomHeight': False, 
+                          'DataSetPattern': False, 
+                          'HeightFrom': 0, 
+                          'LocalPattern': True, 
+                          'Operation': 'Dispense', 
+                          'OverrideHeight': False, 
+                          'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 
+                          'Prototype': 'MC P300 High', 
+                          'ReferencedPattern': '', 
+                          'RowsFirst': False, 
+                          'SectionExpression': '', 
+                          'SelectionInfo': (1,), 
+                          'SetMark': True, 
+                          'Source': False, 
+                          'StartAtMark': False, 
+                          'StartAtSelection': True, 
+                          'UseExpression': False}
         }
 
 
@@ -460,20 +213,29 @@ class LiquidHandlerBiomek:
         处理Biomek的液体转移操作。
 
         """
-        
+        items = []
+
         asp_params = self.aspirate_techniques.get(aspirate_techniques, {})
         dis_params = self.dispense_techniques.get(dispense_techniques, {})
 
+        asp_params['Position'] = source
+        dis_params['Position'] = target
+        asp_params['Volume'] = str(volume)
+        dis_params['Volume'] = str(volume)
+
+        items.append(asp_params)
+        items.append(dis_params)
+
         transfer_params = {
             "Span8": False,
             "Pod": "Pod1",
-            "items": {},                      
+            "items": [],                      
             "Wash": False,
             "Dynamic?": True,
             "AutoSelectActiveWashTechnique": False,
             "ActiveWashTechnique": "",
-            "ChangeTipsBetweenDests": False,
-            "ChangeTipsBetweenSources": True,
+            "ChangeTipsBetweenDests": True,
+            "ChangeTipsBetweenSources": False,
             "DefaultCaption": "",             
             "UseExpression": False,
             "LeaveTipsOn": False,
@@ -490,27 +252,20 @@ class LiquidHandlerBiomek:
             "SplitVolume": False,
             "SplitVolumeCleaning": False,
             "Stop": "Destinations",
-            "TipLocation": "BC1025F",
+            "TipLocation": "BC230",
             "UseCurrentTips": False,    
-            "UseDisposableTips": True,
+            "UseDisposableTips": False,
             "UseFixedTips": False,
             "UseJIT": True,
             "UseMandrelSelection": True,
             "UseProbes": [True, True, True, True, True, True, True, True],
-            "WashCycles": "1",
+            "WashCycles": "4",
             "WashVolume": "110%",
             "Wizard": False
         }
-
-        items: dict = {}
-        items["Source"] = source
-        items["Destination"] = target
-        items["Volume"] = volume 
         transfer_params["items"] = items
-        transfer_params["Solvent"] =  asp_params['Solvent']
+        transfer_params["Solvent"] = 'Water'
         transfer_params["TipLocation"] = tip_rack
-        transfer_params.update(asp_params)
-        transfer_params.update(dis_params)
         self.temp_protocol["steps"].append(transfer_params)
 
         return
@@ -574,6 +329,274 @@ class LiquidHandlerBiomek:
         return
                       
 
+
+if __name__ == "__main__":
+
+    steps_info = ''' 
+    {
+      "steps": [
+        {
+          "step_number": 1,
+          "operation": "transfer",
+          "description": "转移PCR产物或酶促反应液至0.05ml 96孔板中",
+          "parameters": {
+            "source": "P1",
+            "target": "P11",
+            "tip_rack": "BC230",
+            "volume": 50
+          }
+        },
+        {
+          "step_number": 2,
+          "operation": "transfer",
+          "description": "加入2倍体积Bind Beads BC至产物中",
+          "parameters": {
+            "source": "P2",
+            "target": "P11",
+            "tip_rack": "BC230",
+            "volume": 100
+          }
+        },
+        {
+          "step_number": 3,
+          "operation": "move_labware",
+          "description": "移动P11至Orbital1用于振荡混匀",
+          "parameters": {
+            "source": "P11",
+            "target": "Orbital1"
+          }
+        },
+        {
+          "step_number": 4,
+          "operation": "oscillation",
+          "description": "在Orbital1上振荡混匀Bind Beads BC与PCR产物（700-900rpm，300秒）",
+          "parameters": {
+            "rpm": 800,
+            "time": 300
+          }
+        },
+        {
+          "step_number": 5,
+          "operation": "move_labware",
+          "description": "移动混匀后的板回P11",
+          "parameters": {
+            "source": "Orbital1",
+            "target": "P11"
+          }
+        },
+        {
+          "step_number": 6,
+          "operation": "move_labware",
+          "description": "将P11移动到磁力架（P12）吸附3分钟",
+          "parameters": {
+            "source": "P11",
+            "target": "P12"
+          }
+        },
+        {
+          "step_number": 7,
+          "operation": "incubation",
+          "description": "磁力架上室温静置3分钟完成吸附",
+          "parameters": {
+            "time": 180
+          }
+        },
+        {
+          "step_number": 8,
+          "operation": "transfer",
+          "description": "去除上清液至废液槽",
+          "parameters": {
+            "source": "P12",
+            "target": "P22",
+            "tip_rack": "BC230",
+            "volume": 150
+          }
+        },
+        {
+          "step_number": 9,
+          "operation": "transfer",
+          "description": "加入300-500μl 75%乙醇清洗",
+          "parameters": {
+            "source": "P3",
+            "target": "P12",
+            "tip_rack": "BC230",
+            "volume": 400
+          }
+        },
+        {
+          "step_number": 10,
+          "operation": "move_labware",
+          "description": "移动清洗板到Orbital1进行振荡",
+          "parameters": {
+            "source": "P12",
+            "target": "Orbital1"
+          }
+        },
+        {
+          "step_number": 11,
+          "operation": "oscillation",
+          "description": "乙醇清洗液振荡混匀（700-900rpm, 45秒）",
+          "parameters": {
+            "rpm": 800,
+            "time": 45
+          }
+        },
+        {
+          "step_number": 12,
+          "operation": "move_labware",
+          "description": "振荡后将板移回磁力架P12吸附",
+          "parameters": {
+            "source": "Orbital1",
+            "target": "P12"
+          }
+        },
+        {
+          "step_number": 13,
+          "operation": "incubation",
+          "description": "吸附3分钟",
+          "parameters": {
+            "time": 180
+          }
+        },
+        {
+          "step_number": 14,
+          "operation": "transfer",
+          "description": "去除乙醇上清液至废液槽",
+          "parameters": {
+            "source": "P12",
+            "target": "P22",
+            "tip_rack": "BC230",
+            "volume": 400
+          }
+        },
+        {
+          "step_number": 15,
+          "operation": "transfer",
+          "description": "第二次加入300-500μl 75%乙醇清洗",
+          "parameters": {
+            "source": "P3",
+            "target": "P12",
+            "tip_rack": "BC230",
+            "volume": 400
+          }
+        },
+        {
+          "step_number": 16,
+          "operation": "move_labware",
+          "description": "再次移动清洗板到Orbital1振荡",
+          "parameters": {
+            "source": "P12",
+            "target": "Orbital1"
+          }
+        },
+        {
+          "step_number": 17,
+          "operation": "oscillation",
+          "description": "再次乙醇清洗液振荡混匀（700-900rpm, 45秒）",
+          "parameters": {
+            "rpm": 800,
+            "time": 45
+          }
+        },
+        {
+          "step_number": 18,
+          "operation": "move_labware",
+          "description": "振荡后板送回磁力架P12吸附",
+          "parameters": {
+            "source": "Orbital1",
+            "target": "P12"
+          }
+        },
+        {
+          "step_number": 19,
+          "operation": "incubation",
+          "description": "再次吸附3分钟",
+          "parameters": {
+            "time": 180
+          }
+        },
+        {
+          "step_number": 20,
+          "operation": "transfer",
+          "description": "去除乙醇上清液至废液槽",
+          "parameters": {
+            "source": "P12",
+            "target": "P22",
+            "tip_rack": "BC230",
+            "volume": 400
+          }
+        },
+        {
+          "step_number": 21,
+          "operation": "incubation",
+          "description": "空气干燥15分钟",
+          "parameters": {
+            "time": 900
+          }
+        },
+        {
+          "step_number": 22,
+          "operation": "transfer",
+          "description": "加30-50μl Elution Buffer洗脱",
+          "parameters": {
+            "source": "P4",
+            "target": "P12",
+            "tip_rack": "BC230",
+            "volume": 40
+          }
+        },
+        {
+          "step_number": 23,
+          "operation": "move_labware",
+          "description": "移动到Orbital1振荡混匀（60秒）",
+          "parameters": {
+            "source": "P12",
+            "target": "Orbital1"
+          }
+        },
+        {
+          "step_number": 24,
+          "operation": "oscillation",
+          "description": "Elution Buffer振荡混匀（700-900rpm, 60秒）",
+          "parameters": {
+            "rpm": 800,
+            "time": 60
+          }
+        },
+        {
+          "step_number": 25,
+          "operation": "move_labware",
+          "description": "振荡后送回磁力架P12",
+          "parameters": {
+            "source": "Orbital1",
+            "target": "P12"
+          }
+        },
+        {
+          "step_number": 26,
+          "operation": "incubation",
+          "description": "室温静置3分钟（洗脱反应）",
+          "parameters": {
+            "time": 180
+          }
+        },
+        {
+          "step_number": 27,
+          "operation": "transfer",
+          "description": "将上清液（DNA）转移到新板（P13）",
+          "parameters": {
+            "source": "P12",
+            "target": "P13",
+            "tip_rack": "BC230",
+            "volume": 40
+          }
+        }
+      ]
+    }
+    '''
+
+
+
 labware_with_liquid = '''
 [    {
         "id": "stock plate on P1",
@@ -599,181 +622,191 @@ labware_with_liquid = '''
         ]
     },
         {
-        "id": "Tip Rack BC230 on TL3",
-        "parent": "deck",
-        "slot_on_deck": "TL3",
-        "class_name": "BC230",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
-        {
-        "id": "Tip Rack BC230 on TL4",
-        "parent": "deck",
-        "slot_on_deck": "TL4",
-        "class_name": "BC230",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
-        {
-        "id": "Tip Rack BC230 on TL5",
-        "parent": "deck",
-        "slot_on_deck": "TL5",
-        "class_name": "BC230",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
+            "id": "Tip Rack BC230 TL2",
+            "parent": "deck",
+            "slot_on_deck": "TL2",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
             {
-        "id": "Tip Rack BC230 on P5",
-        "parent": "deck",
-        "slot_on_deck": "P5",
-        "class_name": "BC230",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
+            "id": "Tip Rack BC230 on TL3",
+            "parent": "deck",
+            "slot_on_deck": "TL3",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
+            {
+            "id": "Tip Rack BC230 on TL4",
+            "parent": "deck",
+            "slot_on_deck": "TL4",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
+            {
+            "id": "Tip Rack BC230 on TL5",
+            "parent": "deck",
+            "slot_on_deck": "TL5",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
                 {
-        "id": "Tip Rack BC230 on P6",
-        "parent": "deck",
-        "slot_on_deck": "P6",
-        "class_name": "BC230",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
+            "id": "Tip Rack BC230 on P5",
+            "parent": "deck",
+            "slot_on_deck": "P5",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
                     {
-        "id": "Tip Rack BC230 on P7",
-        "parent": "deck",
-        "slot_on_deck": "P7",
-        "class_name": "BC230",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
-    {
-        "id": "Tip Rack BC230 on P8",
-        "parent": "deck",
-        "slot_on_deck": "P8",
-        "class_name": "BC230",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
+            "id": "Tip Rack BC230 on P6",
+            "parent": "deck",
+            "slot_on_deck": "P6",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
+                        {
+            "id": "Tip Rack BC230 on P7",
+            "parent": "deck",
+            "slot_on_deck": "P7",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
         {
-        "id": "Tip Rack BC230 P16",
-        "parent": "deck",
-        "slot_on_deck": "P16",
-        "class_name": "BC230",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
-    {
-        "id": "stock plate on 4",
-        "parent": "deck",
-        "slot_on_deck": "P2",
-        "class_name": "nest_12_reservoir_15ml",
-        "liquid_type": [
-            "bind beads"
-        ],
-        "liquid_volume": [10000],
-        "liquid_input_wells": [
-            "A1"
-        ]
-    },
-        {
-        "id": "stock plate on P2",
-        "parent": "deck",
-        "slot_on_deck": "P2",
-        "class_name": "nest_12_reservoir_15ml",
-        "liquid_type": [
-            "bind beads"
-        ],
-        "liquid_volume": [10000],
-        "liquid_input_wells": [
-            "A1"
-        ]
-    },
-        {
-        "id": "stock plate on P3",
-        "parent": "deck",
-        "slot_on_deck": "P3",
-        "class_name": "nest_12_reservoir_15ml",
-        "liquid_type": [
-            "ethyl alcohol"
-        ],
-        "liquid_volume": [10000],
-        "liquid_input_wells": [
-            "A1"
-        ]
-    },
-
-        {
-        "id": "oscillation",
-        "parent": "deck",
-        "slot_on_deck": "Orbital1",
-        "class_name": "Orbital",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
-        {
-        "id": "working plate on P11",
-        "parent": "deck",
-        "slot_on_deck": "P11",
-        "class_name": "NEST 2ml Deep Well Plate",
-        "liquid_type": [
-        ],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
+            "id": "Tip Rack BC230 on P8",
+            "parent": "deck",
+            "slot_on_deck": "P8",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
             {
-        "id": "magnetics module on P12",
-        "parent": "deck",
-        "slot_on_deck": "P12",
-        "class_name": "magnetics module",
-        "liquid_type": [],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
-                {
-        "id": "working plate on P13",
-        "parent": "deck",
-        "slot_on_deck": "P13",
-        "class_name": "NEST 2ml Deep Well Plate",
-        "liquid_type": [
-        ],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    },
+            "id": "Tip Rack BC230 P16",
+            "parent": "deck",
+            "slot_on_deck": "P16",
+            "class_name": "BC230",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
         {
-        "id": "waste on P22",
-        "parent": "deck",
-        "slot_on_deck": "P22",
-        "class_name": "nest_1_reservoir_195ml",
-        "liquid_type": [
-        ],
-        "liquid_volume": [],
-        "liquid_input_wells": [
-        ]
-    }
-]
-
-'''
+            "id": "stock plate on 4",
+            "parent": "deck",
+            "slot_on_deck": "P2",
+            "class_name": "nest_12_reservoir_15ml",
+            "liquid_type": [
+                "bind beads"
+            ],
+            "liquid_volume": [10000],
+            "liquid_input_wells": [
+                "A1"
+            ]
+        },
+            {
+            "id": "stock plate on P2",
+            "parent": "deck",
+            "slot_on_deck": "P2",
+            "class_name": "nest_12_reservoir_15ml",
+            "liquid_type": [
+                "bind beads"
+            ],
+            "liquid_volume": [10000],
+            "liquid_input_wells": [
+                "A1"
+            ]
+        },
+            {
+            "id": "stock plate on P3",
+            "parent": "deck",
+            "slot_on_deck": "P3",
+            "class_name": "nest_12_reservoir_15ml",
+            "liquid_type": [
+                "ethyl alcohol"
+            ],
+            "liquid_volume": [10000],
+            "liquid_input_wells": [
+                "A1"
+            ]
+        },
+    
+            {
+            "id": "oscillation",
+            "parent": "deck",
+            "slot_on_deck": "Orbital1",
+            "class_name": "Orbital",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
+            {
+            "id": "working plate on P11",
+            "parent": "deck",
+            "slot_on_deck": "P11",
+            "class_name": "NEST 2ml Deep Well Plate",
+            "liquid_type": [
+            ],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
+                {
+            "id": "magnetics module on P12",
+            "parent": "deck",
+            "slot_on_deck": "P12",
+            "class_name": "magnetics module",
+            "liquid_type": [],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
+                    {
+            "id": "working plate on P13",
+            "parent": "deck",
+            "slot_on_deck": "P13",
+            "class_name": "NEST 2ml Deep Well Plate",
+            "liquid_type": [
+            ],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        },
+            {
+            "id": "waste on P22",
+            "parent": "deck",
+            "slot_on_deck": "P22",
+            "class_name": "nest_1_reservoir_195ml",
+            "liquid_type": [
+            ],
+            "liquid_volume": [],
+            "liquid_input_wells": [
+            ]
+        }
+    ]
+    
+    '''
 
 
 
@@ -792,7 +825,6 @@ for step in input_steps['steps']:
     if step['operation'] != 'transfer':
         continue
     parameters = step['parameters']
-    
 
     handler.transfer_biomek(source=parameters['source'],
                             target=parameters['target'],
@@ -801,6 +833,5 @@ for step in input_steps['steps']:
                             aspirate_techniques='MC P300 high',
                             dispense_techniques='MC P300 high'
                             )
-    
-print(json.dumps(handler.temp_protocol['steps'],indent=4, ensure_ascii=False))
 
+print(json.dumps(handler.temp_protocol, indent=4))