Update 9320

unilabos/devices/liquid_handling/prcxi/prcxi.py

@@ -961,71 +961,303 @@ class PRCXI9300Api:
 
 
 if __name__ == "__main__":
+    # # Example usage
+    # # 1. 用导出的json，给每个T1 T2板子设定相应的物料，如果是孔板和枪头盒，要对应区分
+    # # 2. 设计一个单点动作流程，可以跑
+    # # 3.
+    # deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
+    # plate1 = PRCXI9300Container(name="rackT1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    # plate1.load_state({
+    #     "Material": {
+    #         "uuid": "80652665f6a54402b2408d50b40398df",
+    #         "Co#de": "ZX-001-1000",
+    #         "Name": "1000μL Tip头",
+    #         "SummaryName": "1000μL Tip头",
+    #         "PipetteHeight": 100,
+    #         "materialEnum": 1
+    #     }
+    # })
+
+    # plate2 = PRCXI9300Container(name="plateT2", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    # plate2.load_state({
+    #     "Material": {
+    #         "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+    #     }
+    # })
+
+    # plate3 = PRCXI9300Container(name="plateT3", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    # plate3.load_state({
+    #     "Material": {
+    #         "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+    #     }
+    # })
+
+    # plate4 = PRCXI9300Container(name="rackT4", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    # plate4.load_state({
+    #     "Material": {
+    #         "uuid": "80652665f6a54402b2408d50b40398df",
+    #         "Code": "ZX-001-1000",
+    #         "Name": "1000μL Tip头",
+    #         "SummaryName": "1000μL Tip头",
+    #         "PipetteHeight": 100,
+    #         "materialEnum": 1
+    #     }
+    # })
+
+    # plate5 = PRCXI9300Container(name="plateT5", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    # plate5.load_state({
+    #     "Material": {
+    #         "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+    #     }
+    # })
+    # plate6 = PRCXI9300Trash(name="trash", size_x=50, size_y=500, size_z=10, category="trash")
+    # plate6.load_state({
+    #     "Material": {
+    #         "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+    #     }
+    # })
+
+    # from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul
+    # from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat 
+
+    # tip_rack = tipone_96_tiprack_200ul("TipRack")
+    # well_containers = corning_96_wellplate_360ul_flat("Plate")
+    # # from pprint import pprint
+    # # pprint(well_containers.children)
+    # plate1.assign_child_resource(tip_rack, location=Coordinate(0, 0, 0))
+    # plate2.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+
+    # deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate2, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate3, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate4, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate5, location=Coordinate(0, 0, 0))
+    # deck.assign_child_resource(plate6, location=Coordinate(0, 0, 0))
+
+    # print(plate2)  
+    # # plate_2_liquids = [[(None, 500)]]*96
+
+    # # plate2.set_well_liquids(plate_2_liquids)
+
+
+    # handler = PRCXI9300Handler(deck=deck, host="192.168.3.9", port=9999, timeout=10.0, setup=False, debug=True, matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1")
+    # handler.set_tiprack([tip_rack])  # Set the tip rack for the handler
+    # asyncio.run(handler.setup())  # Initialize the handler and setup the connection
+    # from pylabrobot.resources import set_volume_tracking
+
+    # # from pylabrobot.resources import set_tip_tracking
+    # set_volume_tracking(enabled=True)
+    # plate2.set_well_liquids([("Water", 100)] * plate2.num_items)
+    # asyncio.run(handler.create_protocol(protocol_name="Test Protocol"))  # Initialize the backend and setup the connection
+    # # asyncio.run(handler.pick_up_tips(tip_rack.children[:8],[0,1,2,3,4,5,6,7]))
+
+    # # asyncio.run(handler.aspirate(well_containers.children[:8],[50]*8, [0,1,2,3,4,5,6,7]))
+    # # asyncio.run(handler.dispense(well_containers.children[:8],[50]*8,[0,1,2,3,4,5,6,7]))
+    # # asyncio.run(handler.drop_tips(tip_rack.children[8:16],[0,1,2,3,4,5,6,7]))
+    # # asyncio.run(handler.discard_tips())
+    # # asyncio.run(handler.mix(well_containers.children[:8
+    # # ], mix_time=3, mix_vol=50, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
+    # #print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2))  # Print matrix info
+    # asyncio.run(handler.add_liquid(
+    #     asp_vols=[100]*16,
+    #     dis_vols=[100]*16,
+    #     reagent_sources=well_containers.children[-16:],
+    #     targets=well_containers.children[:16],
+    #     use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
+    #     flow_rates=[None] * 32,
+    #     offsets=[Coordinate(0, 0, 0)] * 32,
+    #     liquid_height=[None] * 16,
+    #     blow_out_air_volume=[None] * 16,
+    #     delays=None,
+    #     mix_time=3,
+    #     mix_vol=50,
+    #     spread="wide",
+    # ))
+
+    # # asyncio.run(handler.remove_liquid(
+    # #     vols=[100]*16,
+    # #     sources=well_containers.children[-16:],
+    # #     waste_liquid=well_containers.children[:16], # 这个有些奇怪，但是好像也只能这么写
+    # #     use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
+    # #     flow_rates=[None] * 32,
+    # #     offsets=[Coordinate(0, 0, 0)] * 32,
+    # #     liquid_height=[None] * 32,
+    # #     blow_out_air_volume=[None] * 32,
+    # #     spread="wide",
+    # # ))
+    # # asyncio.run(handler.transfer_liquid(
+    # #     asp_vols=[100]*16,
+    # #     dis_vols=[100]*16,
+    # #     tip_racks=[tip_rack],
+    # #     sources=well_containers.children[-16:],
+    # #     targets=well_containers.children[:16],
+    # #     use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
+    # #     offsets=[Coordinate(0, 0, 0)] * 32,
+    # #     asp_flow_rates=[None] * 16,
+    # #     dis_flow_rates=[None] * 16,
+    # #     liquid_height=[None] * 32,
+    # #     blow_out_air_volume=[None] * 32,
+    # #     mix_times=3,
+    # #     mix_vol=50,
+    # #     spread="wide",
+    # # ))
+    # print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2))  # Print matrix info
+    # # input("pick_up_tips add step")
+    # #asyncio.run(handler.run_protocol())  # Run the protocol
+    # # input("Running protocol...")
+    # # input("Press Enter to continue...")  # Wait for user input before proceeding
+    # # print("PRCXI9300Handler initialized with deck and host settings.")
+
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     # Example usage
     # 1. 用导出的json，给每个T1 T2板子设定相应的物料，如果是孔板和枪头盒，要对应区分
     # 2. 设计一个单点动作流程，可以跑
     # 3.
     deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
-    plate1 = PRCXI9300Container(name="rackT1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate1 = PRCXI9300Container(name="HPLCPlateT1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
     plate1.load_state({
         "Material": {
-            "uuid": "80652665f6a54402b2408d50b40398df",
+            "uuid": "548bbc3df0d4447586f2c19d2c0c0c55",
-            "Co#de": "ZX-001-1000",
+            "Code": "HPLC01",
-            "Name": "1000μL Tip头",
+            "Name":  "HPLC料盘"
-            "SummaryName": "1000μL Tip头",
-            "PipetteHeight": 100,
-            "materialEnum": 1
         }
     })
 
     plate2 = PRCXI9300Container(name="plateT2", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
     plate2.load_state({
         "Material": {
-            "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
         }
     })
     
+    #储液槽
+
     plate3 = PRCXI9300Container(name="plateT3", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
     plate3.load_state({
         "Material": {
-            "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
         }
     })
 
-    plate4 = PRCXI9300Container(name="rackT4", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate4 = PRCXI9300Trash(name="trash", size_x=50, size_y=50, size_z=10, category="trash", ordering=collections.OrderedDict())
     plate4.load_state({
         "Material": {
-            "uuid": "80652665f6a54402b2408d50b40398df",
+            "uuid": "730067cf07ae43849ddf4034299030e9"
-            "Code": "ZX-001-1000",
-            "Name": "1000μL Tip头",
-            "SummaryName": "1000μL Tip头",
-            "PipetteHeight": 100,
-            "materialEnum": 1
         }
     })
 
     plate5 = PRCXI9300Container(name="plateT5", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
     plate5.load_state({
         "Material": {
-            "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
         }
     })
-    plate6 = PRCXI9300Trash(name="trash", size_x=50, size_y=500, size_z=10, category="trash")
+
+    plate6 = PRCXI9300Container(name="plateT6", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
     plate6.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+
+    plate7 = PRCXI9300Container(name="plateT7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate7.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+
+    plate8 = PRCXI9300Container(name="rackT8", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate8.load_state({
+        "Material": {
+            "uuid": "068b3815e36b4a72a59bae017011b29f",
+            "Code": "ZX-001-10+",
+            "Name": "10μL加长 Tip头"
+        }
+    })
+
+    plate9 = PRCXI9300Container(name="plateT9", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate9.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+
+    plate10 = PRCXI9300Container(name="plateT10", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate10.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+
+
+
+    plate11 = PRCXI9300Container(name="plateT11", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate11.load_state({
         "Material": {
             "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
         }
     })
 
-    from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul
-    from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat 
 
-    tip_rack = tipone_96_tiprack_200ul("TipRack")
+    plate12 = PRCXI9300Container(name="plateT12", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
-    well_containers = corning_96_wellplate_360ul_flat("Plate")
+    plate12.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+
+
+    plate13 = PRCXI9300Container(name="plateT13", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
+    plate13.load_state({
+        "Material": {
+            "uuid": "04211a2dc93547fe9bf6121eac533650"
+        }
+    })
+
+    from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul,opentrons_96_tiprack_10ul
+    from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
+
+    tip_rack = opentrons_96_tiprack_10ul("TipRack")
+    well_containers = nest_96_wellplate_2ml_deep("Plate")
     # from pprint import pprint
     # pprint(well_containers.children)
-    plate1.assign_child_resource(tip_rack, location=Coordinate(0, 0, 0))
+    plate1.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
     plate2.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate3.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate4.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate5.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate6.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate7.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate8.assign_child_resource(tip_rack, location=Coordinate(0, 0, 0))
+    plate9.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate10.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate11.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate12.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
+    plate13.assign_child_resource(well_containers, location=Coordinate(0, 0, 0))
 
     deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
     deck.assign_child_resource(plate2, location=Coordinate(0, 0, 0))
@@ -1033,11 +1265,14 @@ if __name__ == "__main__":
     deck.assign_child_resource(plate4, location=Coordinate(0, 0, 0))
     deck.assign_child_resource(plate5, location=Coordinate(0, 0, 0))
     deck.assign_child_resource(plate6, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate7, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate8, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate9, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate10, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate11, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate12, location=Coordinate(0, 0, 0))
+    deck.assign_child_resource(plate13, location=Coordinate(0, 0, 0))
 
-    print(plate2)  
-    # plate_2_liquids = [[(None, 500)]]*96
-
-    # plate2.set_well_liquids(plate_2_liquids)
 
 
     handler = PRCXI9300Handler(deck=deck, host="192.168.3.9", port=9999, timeout=10.0, setup=False, debug=True, matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1")
@@ -1108,6 +1343,3 @@ if __name__ == "__main__":
     # input("Press Enter to continue...")  # Wait for user input before proceeding
     # print("PRCXI9300Handler initialized with deck and host settings.")
 
-
-
-# 但是怎么丢tip？这个需要手动设置一下？