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This commit is contained in:
Guangxin Zhang
2025-07-17 23:44:59 +08:00
parent 6eaa8e1c63
commit 89808285ed
1 changed files with 14 additions and 13 deletions
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27
unilabos/devices/liquid_handling/prcxi/prcxi.py
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@@ -427,10 +427,7 @@ class PRCXI9300Backend(LiquidHandlerBackend):
async def pick_up_tips(self, ops: List[Pickup], use_channels: List[int] = None): async def pick_up_tips(self, ops: List[Pickup], use_channels: List[int] = None):
"""Pick up tips from the specified resource.""" """Pick up tips from the specified resource."""
if len(ops) != 8:
raise ValueError(f"PRCXI9300Backend pick_up_tips: Expected 8 pickups, got {len(ops)}")
plate_indexes = [] plate_indexes = []
for op in ops: for op in ops:
plate = op.resource.parent.parent plate = op.resource.parent.parent
@@ -451,7 +448,10 @@ class PRCXI9300Backend(LiquidHandlerBackend):
PlateNo = plate_indexes[0] + 1 PlateNo = plate_indexes[0] + 1
hole_col = tip_columns[0] + 1 hole_col = tip_columns[0] + 1
step = self.api_client.Load(dosage=0, plate_no=PlateNo, is_whole_plate=False, hole_row=1, hole_col=hole_col, if self.channel_num == 1:
hole_row = tipspot_index % 8 + 1
step = self.api_client.Load(dosage=0, plate_no=PlateNo, is_whole_plate=False, hole_row=hole_row, hole_col=hole_col,
blending_times=0, balance_height=0, plate_or_hole=f"H{hole_col}-8,T{PlateNo}", blending_times=0, balance_height=0, plate_or_hole=f"H{hole_col}-8,T{PlateNo}",
hole_numbers="1,2,3,4,5,6,7,8") hole_numbers="1,2,3,4,5,6,7,8")
self.steps_todo_list.append(step) self.steps_todo_list.append(step)
@@ -571,9 +571,6 @@ class PRCXI9300Backend(LiquidHandlerBackend):
"""Aspirate liquid from the specified resources.""" """Aspirate liquid from the specified resources."""
if len(ops) != 8:
raise ValueError(f"PRCXI9300Backend aspirate: Expected 8 aspirate, got {len(ops)}")
plate_indexes = [] plate_indexes = []
for op in ops: for op in ops:
plate = op.resource.parent.parent plate = op.resource.parent.parent
@@ -600,7 +597,10 @@ class PRCXI9300Backend(LiquidHandlerBackend):
PlateNo = plate_indexes[0] + 1 PlateNo = plate_indexes[0] + 1
hole_col = tip_columns[0] + 1 hole_col = tip_columns[0] + 1
step = self.api_client.Imbibing(dosage=int(volumes[0]), plate_no=PlateNo, is_whole_plate=False, hole_row=1, if self.channel_num == 1:
hole_row = tipspot_index % 8 + 1
step = self.api_client.Imbibing(dosage=int(volumes[0]), plate_no=PlateNo, is_whole_plate=False, hole_row=hole_row,
hole_col=hole_col, blending_times=0, balance_height=0, hole_col=hole_col, blending_times=0, balance_height=0,
plate_or_hole=f"H{hole_col}-8,T{PlateNo}", hole_numbers="1,2,3,4,5,6,7,8") plate_or_hole=f"H{hole_col}-8,T{PlateNo}", hole_numbers="1,2,3,4,5,6,7,8")
self.steps_todo_list.append(step) self.steps_todo_list.append(step)
@@ -611,9 +611,6 @@ class PRCXI9300Backend(LiquidHandlerBackend):
"""Dispense liquid into the specified resources.""" """Dispense liquid into the specified resources."""
if len(ops) != 8:
raise ValueError(f"PRCXI9300Backend dispense: Expected 8 dispense, got {len(ops)}")
plate_indexes = [] plate_indexes = []
for op in ops: for op in ops:
plate = op.resource.parent.parent plate = op.resource.parent.parent
@@ -640,12 +637,15 @@ class PRCXI9300Backend(LiquidHandlerBackend):
PlateNo = plate_indexes[0] + 1 PlateNo = plate_indexes[0] + 1
hole_col = tip_columns[0] + 1 hole_col = tip_columns[0] + 1
if self.channel_num == 1:
hole_row = tipspot_index % 8 + 1
step = self.api_client.Tapping( step = self.api_client.Tapping(
"Left", "Left",
dosage=int(volumes[0]), dosage=int(volumes[0]),
plate_no=PlateNo, plate_no=PlateNo,
is_whole_plate=False, is_whole_plate=False,
hole_row=1, hole_row=hole_row,
hole_col=hole_col, hole_col=hole_col,
blending_times=0, blending_times=0,
balance_height=0, balance_height=0,
@@ -1278,6 +1278,7 @@ if __name__ == "__main__":
asyncio.run(handler.dispense(plate1.children[3],[10],[1])) asyncio.run(handler.dispense(plate1.children[3],[10],[1]))
asyncio.run(handler.mix(plate1.children[3], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100)) asyncio.run(handler.mix(plate1.children[3], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
asyncio.run(handler.discard_tips()) asyncio.run(handler.discard_tips())
# asyncio.run(handler.discard_tips()) # asyncio.run(handler.discard_tips())
# asyncio.run(handler.mix(well_containers.children[:8 # asyncio.run(handler.mix(well_containers.children[:8
# ], mix_time=3, mix_vol=50, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100)) # ], mix_time=3, mix_vol=50, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))