对于PRCXI9320的transfer_group,一对多和多对多

This commit is contained in:
Guangxin Zhang
2025-09-15 00:29:16 +08:00
parent 1cd07915e7
commit 94cdcbf24e
4 changed files with 844 additions and 254 deletions

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@@ -981,121 +981,119 @@ if __name__ == "__main__":
# 4.
deck = PRCXI9300Deck(name="PRCXI_Deck_9300", size_x=100, size_y=100, size_z=100)
# deck = PRCXI9300Deck(name="PRCXI_Deck_9300", size_x=100, size_y=100, size_z=100)
from pylabrobot.resources.opentrons.tip_racks import opentrons_96_tiprack_300ul,opentrons_96_tiprack_10ul
from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
# from pylabrobot.resources.opentrons.tip_racks import opentrons_96_tiprack_300ul,opentrons_96_tiprack_10ul
# from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
def get_well_container(name: str) -> PRCXI9300Container:
well_containers = corning_96_wellplate_360ul_flat(name).serialize()
plate = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="plate",
ordering=well_containers["ordering"])
plate_serialized = plate.serialize()
plate_serialized["parent_name"] = deck.name
well_containers.update({k: v for k, v in plate_serialized.items() if k not in ["children"]})
new_plate: PRCXI9300Container = PRCXI9300Container.deserialize(well_containers)
return new_plate
# def get_well_container(name: str) -> PRCXI9300Container:
# well_containers = corning_96_wellplate_360ul_flat(name).serialize()
# plate = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="plate",
# ordering=well_containers["ordering"])
# plate_serialized = plate.serialize()
# plate_serialized["parent_name"] = deck.name
# well_containers.update({k: v for k, v in plate_serialized.items() if k not in ["children"]})
# new_plate: PRCXI9300Container = PRCXI9300Container.deserialize(well_containers)
# return new_plate
def get_tip_rack(name: str) -> PRCXI9300Container:
tip_racks = opentrons_96_tiprack_300ul("name").serialize()
tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
ordering=tip_racks["ordering"])
tip_rack_serialized = tip_rack.serialize()
tip_rack_serialized["parent_name"] = deck.name
tip_racks.update({k: v for k, v in tip_rack_serialized.items() if k not in ["children"]})
new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
return new_tip_rack
# def get_tip_rack(name: str) -> PRCXI9300Container:
# tip_racks = opentrons_96_tiprack_300ul("name").serialize()
# tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
# ordering=tip_racks["ordering"])
# tip_rack_serialized = tip_rack.serialize()
# tip_rack_serialized["parent_name"] = deck.name
# tip_racks.update({k: v for k, v in tip_rack_serialized.items() if k not in ["children"]})
# new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
# return new_tip_rack
plate1 = get_tip_rack("RackT1")
plate1.load_state({
"Material": {
"uuid": "076250742950465b9d6ea29a225dfb00",
"Code": "ZX-001-300",
"Name": "300μL Tip头"
}
})
# plate1 = get_tip_rack("RackT1")
# plate1.load_state({
# "Material": {
# "uuid": "076250742950465b9d6ea29a225dfb00",
# "Code": "ZX-001-300",
# "Name": "300μL Tip头"
# }
# })
plate2 = get_well_container("PlateT2")
plate2.load_state({
"Material": {
"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
"Code": "ZX-019-2.2",
"Name": "96深孔板"
}
})
# plate2 = get_well_container("PlateT2")
# plate2.load_state({
# "Material": {
# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
# "Code": "ZX-019-2.2",
# "Name": "96深孔板"
# }
# })
plate3 = PRCXI9300Trash("trash", size_x=50, size_y=100, size_z=10, category="trash")
plate3.load_state({
"Material": {
"uuid": "730067cf07ae43849ddf4034299030e9"
}
})
# plate3 = PRCXI9300Trash("trash", size_x=50, size_y=100, size_z=10, category="trash")
# plate3.load_state({
# "Material": {
# "uuid": "730067cf07ae43849ddf4034299030e9"
# }
# })
plate4 = get_well_container("PlateT4")
plate4.load_state({
"Material": {
"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
"Code": "ZX-019-2.2",
"Name": "96深孔板"
}
})
# plate4 = get_well_container("PlateT4")
# plate4.load_state({
# "Material": {
# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
# "Code": "ZX-019-2.2",
# "Name": "96深孔板"
# }
# })
plate5 = get_well_container("PlateT5")
plate5.load_state({
"Material": {
"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
"Code": "ZX-019-2.2",
"Name": "96深孔板"
}
})
plate6 = get_well_container("PlateT6")
# plate5 = get_well_container("PlateT5")
# plate5.load_state({
# "Material": {
# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
# "Code": "ZX-019-2.2",
# "Name": "96深孔板"
# }
# })
# plate6 = get_well_container("PlateT6")
plate6.load_state({
"Material": {
"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
"Code": "ZX-019-2.2",
"Name": "96深孔板"
}
})
# plate6.load_state({
# "Material": {
# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
# "Code": "ZX-019-2.2",
# "Name": "96深孔板"
# }
# })
deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
deck.assign_child_resource(plate2, location=Coordinate(0, 0, 0))
deck.assign_child_resource(plate3, location=Coordinate(0, 0, 0))
deck.assign_child_resource(plate4, location=Coordinate(0, 0, 0))
deck.assign_child_resource(plate5, location=Coordinate(0, 0, 0))
deck.assign_child_resource(plate6, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate2, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate3, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate4, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate5, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate6, location=Coordinate(0, 0, 0))
# plate_2_liquids = [[('water', 500)]]*96
# # # plate_2_liquids = [[('water', 500)]]*96
# plate2.set_well_liquids(plate_2_liquids)
# # # plate2.set_well_liquids(plate_2_liquids)
handler = PRCXI9300Handler(deck=deck, host="10.181.214.132", port=9999,
timeout=10.0, setup=False, debug=False,
simulator=True,
matrix_id="71593",
channel_num=8, axis="Left") # Initialize the handler with the deck and host settings
# handler = PRCXI9300Handler(deck=deck, host="10.181.214.132", port=9999,
# timeout=10.0, setup=False, debug=False,
# simulator=True,
# matrix_id="71593",
# channel_num=8, axis="Left") # Initialize the handler with the deck and host settings
# plate_2_liquids = handler.set_group("water", plate2.children[:8], [200]*8)
# plate5_liquids = handler.set_group("master_mix", plate5.children[:8], [100]*8)
plate_2_liquids = handler.set_group("water", plate2.children[:8], [200]*8)
plate5_liquids = handler.set_group("master_mix", plate5.children[:8], [100]*8)
handler.set_tiprack([plate1])
asyncio.run(handler.setup()) # Initialize the handler and setup the connection
from pylabrobot.resources import set_volume_tracking
# handler.set_tiprack([plate1])
# asyncio.run(handler.setup()) # Initialize the handler and setup the connection
# from pylabrobot.resources import set_volume_tracking
# from pylabrobot.resources import set_tip_tracking
set_volume_tracking(enabled=True)
# set_volume_tracking(enabled=True)
# from unilabos.resources.graphio import *
# # A = tree_to_list([resource_plr_to_ulab(deck)])
# # with open("deck_9300_new.json", "w", encoding="utf-8") as f:
# # json.dump(A, f, indent=4, ensure_ascii=False)
asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
asyncio.run(handler.transfer_group("water", "master_mix", 100)) # Reset tip tracking
# asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
# asyncio.run(handler.transfer_group("water", "master_mix", 100)) # Reset tip tracking
# asyncio.run(handler.pick_up_tips(plate1.children[:8],[0,1,2,3,4,5,6,7]))
# print(plate1.children[:8])
@@ -1166,164 +1164,172 @@ if __name__ == "__main__":
### 9320 ###
# deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
# from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul,opentrons_96_tiprack_10ul
# from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
from pylabrobot.resources.opentrons.tip_racks import tipone_96_tiprack_200ul,opentrons_96_tiprack_10ul
from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
# def get_well_container(name: str) -> PRCXI9300Container:
# well_containers = corning_96_wellplate_360ul_flat(name).serialize()
# plate = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="plate",
# ordering=collections.OrderedDict())
# plate_serialized = plate.serialize()
# plate_serialized["parent_name"] = deck.name
# well_containers.update({k: v for k, v in plate_serialized.items() if k not in ["children"]})
# new_plate: PRCXI9300Container = PRCXI9300Container.deserialize(well_containers)
# return new_plate
def get_well_container(name: str) -> PRCXI9300Container:
well_containers = corning_96_wellplate_360ul_flat(name).serialize()
plate = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="plate",
ordering=collections.OrderedDict())
plate_serialized = plate.serialize()
plate_serialized["parent_name"] = deck.name
well_containers.update({k: v for k, v in plate_serialized.items() if k not in ["children"]})
new_plate: PRCXI9300Container = PRCXI9300Container.deserialize(well_containers)
return new_plate
# def get_tip_rack(name: str) -> PRCXI9300Container:
# tip_racks = opentrons_96_tiprack_10ul("name").serialize()
# tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
# ordering=collections.OrderedDict())
# tip_rack_serialized = tip_rack.serialize()
# tip_rack_serialized["parent_name"] = deck.name
# tip_racks.update({k: v for k, v in tip_rack_serialized.items() if k not in ["children"]})
# new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
# return new_tip_rack
def get_tip_rack(name: str) -> PRCXI9300Container:
tip_racks = opentrons_96_tiprack_10ul("name").serialize()
tip_rack = PRCXI9300Container(name=name, size_x=50, size_y=50, size_z=10, category="tip_rack",
ordering=collections.OrderedDict())
tip_rack_serialized = tip_rack.serialize()
tip_rack_serialized["parent_name"] = deck.name
tip_racks.update({k: v for k, v in tip_rack_serialized.items() if k not in ["children"]})
new_tip_rack: PRCXI9300Container = PRCXI9300Container.deserialize(tip_racks)
return new_tip_rack
# plate1 = get_well_container("HPLCPlateT1")
# plate1.load_state({
# "Material": {
# "uuid": "548bbc3df0d4447586f2c19d2c0c0c55",
# "Code": "HPLC01",
# "Name": "HPLC料盘"
# }
# })
# plate2 = get_well_container("PlateT2")
# plate2.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650",
# }
# })
# plate3 = get_well_container("PlateT3")
# plate3.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650",
# }
# })
# trash = PRCXI9300Trash(name="trash", size_x=50, size_y=50, size_z=10, category="trash")
# trash.load_state({
# "Material": {
# "uuid": "730067cf07ae43849ddf4034299030e9"
# }
# })
# plate5 = get_well_container("PlateT5")
# plate5.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650",
# }
# })
# plate6 = get_well_container("PlateT6")
# plate6.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650"
# }
# })
# plate7 = PRCXI9300Container(name="plateT7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
# plate7.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650"
# }
# })
# plate8 = get_tip_rack("RackT8")
# plate8.load_state({
# "Material": {
# "uuid": "068b3815e36b4a72a59bae017011b29f",
# "Code": "ZX-001-10+",
# "Name": "10μL加长 Tip头"
# }
# })
# plate9 = get_well_container("PlateT9")
# plate9.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650"
# }
# })
# plate10 = get_well_container("PlateT10")
# plate10.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650"
# }
# })
# plate11 = get_well_container("PlateT11")
# plate11.load_state({
# "Material": {
# "uuid": "57b1e4711e9e4a32b529f3132fc5931f",
# }
# })
# plate12 = get_well_container("PlateT12")
# plate12.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650"
# }
# })
# plate13 = get_well_container("PlateT13")
# plate13.load_state({
# "Material": {
# "uuid": "04211a2dc93547fe9bf6121eac533650"
# }
# })
plate1 = get_well_container("HPLCPlateT1")
plate1.load_state({
"Material": {
"uuid": "548bbc3df0d4447586f2c19d2c0c0c55",
"Code": "HPLC01",
"Name": "HPLC料盘"
}
})
plate2 = get_well_container("PlateT2")
plate2.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650",
}
})
plate3 = get_well_container("PlateT3")
plate3.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650",
}
})
trash = PRCXI9300Trash(name="trash", size_x=50, size_y=50, size_z=10, category="trash")
trash.load_state({
"Material": {
"uuid": "730067cf07ae43849ddf4034299030e9"
}
})
plate5 = get_well_container("PlateT5")
plate5.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650",
}
})
plate6 = get_well_container("PlateT6")
plate6.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650"
}
})
plate7 = PRCXI9300Container(name="plateT7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
plate7.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650"
}
})
plate8 = get_tip_rack("RackT8")
plate8.load_state({
"Material": {
"uuid": "068b3815e36b4a72a59bae017011b29f",
"Code": "ZX-001-10+",
"Name": "10μL加长 Tip头"
}
})
plate9 = get_well_container("PlateT9")
plate9.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650"
}
})
plate10 = get_well_container("PlateT10")
plate10.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650"
}
})
plate11 = get_well_container("PlateT11")
plate11.load_state({
"Material": {
"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
}
})
plate12 = get_well_container("PlateT12")
plate12.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650"
}
})
plate13 = get_well_container("PlateT13")
plate13.load_state({
"Material": {
"uuid": "04211a2dc93547fe9bf6121eac533650"
}
})
# # container_for_nothing = PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
# container_for_nothing = PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict())
# deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing2", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# deck.assign_child_resource(trash, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing3", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing4", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate8, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing5", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing6", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate11, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing8", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(plate1, location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing1", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing2", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(trash, location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing3", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing4", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(plate8, location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing5", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing6", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(plate11, location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing7", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
deck.assign_child_resource(PRCXI9300Container(name="container_for_nothing8", size_x=50, size_y=50, size_z=10, category="plate", ordering=collections.OrderedDict()), location=Coordinate(0, 0, 0))
# handler = PRCXI9300Handler(deck=deck, host="10.181.102.13", port=9999,
# timeout=10.0, setup=False, debug=False,
# matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1",
# channel_num=8, axis="Right") # Initialize the handler with the deck and host settings
handler = PRCXI9300Handler(deck=deck, host="172.21.5.75", port=9999,
timeout=10.0, setup=False, debug=True,
matrix_id="c1d0d5dc-40f2-4f24-97ac-9cc49c68496c",
channel_num=1, axis="Left",simulator=True) # Initialize the handler with the deck and host settings
# handler.set_tiprack([plate8]) # Set the tip rack for the handler
# asyncio.run(handler.setup()) # Initialize the handler and setup the connection
# from pylabrobot.resources import set_volume_tracking
# # from pylabrobot.resources import set_tip_tracking
# set_volume_tracking(enabled=True)
handler.set_tiprack([plate8]) # Set the tip rack for the handler
asyncio.run(handler.setup()) # Initialize the handler and setup the connection
from pylabrobot.resources import set_volume_tracking
# from pylabrobot.resources import set_tip_tracking
set_volume_tracking(enabled=True)
# plate11.set_well_liquids([("Water", 100) if (i % 8 == 0 and i // 8 < 6) else (None, 100) for i in range(96)]) # Set liquids for every 8 wells in plate8
plate_2_liquids = handler.set_group("water", [plate2.children[0]], [300])
#print(plate_2_liquids)
plate5_liquids = handler.set_group("master_mix", plate5.children[:23], [100]*23)
#print(plate5_liquids)
# from unilabos.resources.graphio import *
# plate11.set_well_liquids([("Water", 100) if (i % 8 == 0 and i // 8 < 6) else (None, 100) for i in range(96)]) # Set liquids for every 8 wells in plate8
from unilabos.resources.graphio import *
# A = tree_to_list([resource_plr_to_ulab(deck)])
# # with open("deck.json", "w", encoding="utf-8") as f:
# # json.dump(A, f, indent=4, ensure_ascii=False)
# print(plate11.get_well(0).tracker.get_used_volume())
# asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
# # asyncio.run(handler.pick_up_tips([plate8.children[8]],[0]))
# # print(plate8.children[8])
# # # asyncio.run(handler.run_protocol())
# # asyncio.run(handler.aspirate([plate11.children[0]],[10], [0]))
# # print(plate11.children[0])
# # # asyncio.run(handler.run_protocol())
# # asyncio.run(handler.dispense([plate1.children[0]],[10],[0]))
# # print(plate1.children[0])
# # # asyncio.run(handler.run_protocol())
# # asyncio.run(handler.mix([plate1.children[0]], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
# # print(plate1.children[0])
# # asyncio.run(handler.discard_tips())
asyncio.run(handler.transfer_group("water", "master_mix", 10)) # Reset tip tracking
# asyncio.run(handler.pick_up_tips([plate8.children[8]],[0]))
# print(plate8.children[8])
# asyncio.run(handler.run_protocol())
# asyncio.run(handler.aspirate([plate11.children[0]],[10], [0]))
# print(plate11.children[0])
# # asyncio.run(handler.run_protocol())
# asyncio.run(handler.dispense([plate1.children[0]],[10],[0]))
# print(plate1.children[0])
# # asyncio.run(handler.run_protocol())
# asyncio.run(handler.mix([plate1.children[0]], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
# print(plate1.children[0])
# asyncio.run(handler.discard_tips([0]))
# asyncio.run(handler.add_liquid(
# asp_vols=[10]*7,
@@ -1341,10 +1347,7 @@ if __name__ == "__main__":
# spread="custom",
# ))
# asyncio.run(handler.run_protocol()) # Run the protocol
# asyncio.run(handler.run_protocol()) # Run the protocol
@@ -1413,7 +1416,7 @@ if __name__ == "__main__":
# # # ))
# # print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2)) # Print matrix info
# # # input("pick_up_tips add step")
# # #asyncio.run(handler.run_protocol()) # Run the protocol
#asyncio.run(handler.run_protocol()) # Run the protocol
# # # input("Running protocol...")
# # # input("Press Enter to continue...") # Wait for user input before proceeding
# # # print("PRCXI9300Handler initialized with deck and host settings.")