From 9b3377aedb68822a06dab0a751c6db4db908d03f Mon Sep 17 00:00:00 2001 From: qxw138 Date: Sun, 8 Jun 2025 15:52:20 +0800 Subject: [PATCH] Update biomek_test.py --- .../devices/liquid_handling/biomek_test.py | 888 +++++++++--------- 1 file changed, 442 insertions(+), 446 deletions(-) diff --git a/unilabos/devices/liquid_handling/biomek_test.py b/unilabos/devices/liquid_handling/biomek_test.py index 185d47b5..25e669d0 100644 --- a/unilabos/devices/liquid_handling/biomek_test.py +++ b/unilabos/devices/liquid_handling/biomek_test.py @@ -276,14 +276,14 @@ class LiquidHandlerBiomek: "Class": f"LabwareClasses\\{class_name}", "DataSets": {"Volume": {}}, "RuntimeDataSets": {"Volume": {}}, - "EvalAmounts": (float(liquid_volume[0]),) if liquid_volume else (0.0,), + "EvalAmounts": (float(liquid_volume[0]),) if liquid_volume else (0,), "Nominal": False, "EvalLiquids": (liquid_type[0],) if liquid_type else ("Water",) } elif instrument_type == "plate_96": # 96孔板类型配置 - volume_per_well = float(liquid_volume[0]) if liquid_volume else 500.0 + volume_per_well = float(liquid_volume[0]) if liquid_volume else 0 liquid_per_well = liquid_type[0] if liquid_type else "Water" config = { @@ -473,452 +473,448 @@ if __name__ == "__main__": print("包含: 仪器设置 + 完整实验步骤") # 完整的步骤信息（从biomek.py复制） - steps_info = ''' + steps_info = { + "steps": [ { - "steps": [ - { - "step_number": 1, - "operation": "transfer", - "description": "转移PCR产物或酶促反应液至0.05ml 96孔板中", - "parameters": { - "source": "P1", - "target": "P11", - "tip_rack": "BC230", - "volume": 50 - } - }, - { - "step_number": 2, - "operation": "transfer", - "description": "加入2倍体积Bind Beads BC至产物中", - "parameters": { - "source": "P2", - "target": "P11", - "tip_rack": "BC230", - "volume": 100 - } - }, - { - "step_number": 3, - "operation": "move_labware", - "description": "移动P11至Orbital1用于振荡混匀", - "parameters": { - "source": "P11", - "target": "Orbital1" - } - }, - { - "step_number": 4, - "operation": "oscillation", - "description": "在Orbital1上振荡混匀Bind Beads BC与PCR产物（700-900rpm，300秒）", - "parameters": { - "rpm": 800, - "time": 300 - } - }, - { - "step_number": 5, - "operation": "move_labware", - "description": "移动混匀后的板回P11", - "parameters": { - "source": "Orbital1", - "target": "P11" - } - }, - { - "step_number": 6, - "operation": "move_labware", - "description": "将P11移动到磁力架（P12）吸附3分钟", - "parameters": { - "source": "P11", - "target": "P12" - } - }, - { - "step_number": 7, - "operation": "incubation", - "description": "磁力架上室温静置3分钟完成吸附", - "parameters": { - "time": 180 - } - }, - { - "step_number": 8, - "operation": "transfer", - "description": "去除上清液至废液槽", - "parameters": { - "source": "P12", - "target": "P22", - "tip_rack": "BC230", - "volume": 150 - } - }, - { - "step_number": 9, - "operation": "transfer", - "description": "加入300-500μl 75%乙醇清洗", - "parameters": { - "source": "P3", - "target": "P12", - "tip_rack": "BC230", - "volume": 400 - } - }, - { - "step_number": 10, - "operation": "move_labware", - "description": "移动清洗板到Orbital1进行振荡", - "parameters": { - "source": "P12", - "target": "Orbital1" - } - }, - { - "step_number": 11, - "operation": "oscillation", - "description": "乙醇清洗液振荡混匀（700-900rpm, 45秒）", - "parameters": { - "rpm": 800, - "time": 45 - } - }, - { - "step_number": 12, - "operation": "move_labware", - "description": "振荡后将板移回磁力架P12吸附", - "parameters": { - "source": "Orbital1", - "target": "P12" - } - }, - { - "step_number": 13, - "operation": "incubation", - "description": "吸附3分钟", - "parameters": { - "time": 180 - } - }, - { - "step_number": 14, - "operation": "transfer", - "description": "去除乙醇上清液至废液槽", - "parameters": { - "source": "P12", - "target": "P22", - "tip_rack": "BC230", - "volume": 400 - } - }, - { - "step_number": 15, - "operation": "transfer", - "description": "第二次加入300-500μl 75%乙醇清洗", - "parameters": { - "source": "P3", - "target": "P12", - "tip_rack": "BC230", - "volume": 400 - } - }, - { - "step_number": 16, - "operation": "move_labware", - "description": "再次移动清洗板到Orbital1振荡", - "parameters": { - "source": "P12", - "target": "Orbital1" - } - }, - { - "step_number": 17, - "operation": "oscillation", - "description": "再次乙醇清洗液振荡混匀（700-900rpm, 45秒）", - "parameters": { - "rpm": 800, - "time": 45 - } - }, - { - "step_number": 18, - "operation": "move_labware", - "description": "振荡后板送回磁力架P12吸附", - "parameters": { - "source": "Orbital1", - "target": "P12" - } - }, - { - "step_number": 19, - "operation": "incubation", - "description": "再次吸附3分钟", - "parameters": { - "time": 180 - } - }, - { - "step_number": 20, - "operation": "transfer", - "description": "去除乙醇上清液至废液槽", - "parameters": { - "source": "P12", - "target": "P22", - "tip_rack": "BC230", - "volume": 400 - } - }, - { - "step_number": 21, - "operation": "incubation", - "description": "空气干燥15分钟", - "parameters": { - "time": 900 - } - }, - { - "step_number": 22, - "operation": "transfer", - "description": "加30-50μl Elution Buffer洗脱", - "parameters": { - "source": "P4", - "target": "P12", - "tip_rack": "BC230", - "volume": 40 - } - }, - { - "step_number": 23, - "operation": "move_labware", - "description": "移动到Orbital1振荡混匀（60秒）", - "parameters": { - "source": "P12", - "target": "Orbital1" - } - }, - { - "step_number": 24, - "operation": "oscillation", - "description": "Elution Buffer振荡混匀（700-900rpm, 60秒）", - "parameters": { - "rpm": 800, - "time": 60 - } - }, - { - "step_number": 25, - "operation": "move_labware", - "description": "振荡后送回磁力架P12", - "parameters": { - "source": "Orbital1", - "target": "P12" - } - }, - { - "step_number": 26, - "operation": "incubation", - "description": "室温静置3分钟（洗脱反应）", - "parameters": { - "time": 180 - } - }, - { - "step_number": 27, - "operation": "transfer", - "description": "将上清液（DNA）转移到新板（P13）", - "parameters": { - "source": "P12", - "target": "P13", - "tip_rack": "BC230", - "volume": 40 - } - } - ] + "step_number": 1, + "operation": "transfer", + "description": "转移PCR产物或酶促反应液至0.5ml 96孔板中", + "parameters": { + "source": "P1", + "target": "P11", + "tip_rack": "BC230", + "volume": 50 + } + }, + { + "step_number": 2, + "operation": "transfer", + "description": "加入2倍体积的Bind Beads BC至产物中", + "parameters": { + "source": "P2", + "target": "P11", + "tip_rack": "BC230", + "volume": 100 + } + }, + { + "step_number": 3, + "operation": "oscillation", + "description": "振荡混匀300秒", + "parameters": { + "rpm": 800, + "time": 300 + } + }, + { + "step_number": 4, + "operation": "move_labware", + "description": "转移至96孔磁力架上吸附3分钟", + "parameters": { + "source": "P11", + "target": "P12" + } + }, + { + "step_number": 5, + "operation": "incubation", + "description": "吸附3分钟", + "parameters": { + "time": 180 + } + }, + { + "step_number": 6, + "operation": "transfer", + "description": "吸弃或倒除上清液", + "parameters": { + "source": "P12", + "target": "P22", + "tip_rack": "BC230", + "volume": 150 + } + }, + { + "step_number": 7, + "operation": "transfer", + "description": "加入300-500μl 75%乙醇", + "parameters": { + "source": "P3", + "target": "P12", + "tip_rack": "BC230", + "volume": 400 + } + }, + { + "step_number": 8, + "operation": "move_labware", + "description": "移动至振荡器进行振荡混匀", + "parameters": { + "source": "P12", + "target": "Orbital1" + } + }, + { + "step_number": 9, + "operation": "oscillation", + "description": "振荡混匀60秒", + "parameters": { + "rpm": 800, + "time": 60 + } + }, + { + "step_number": 10, + "operation": "move_labware", + "description": "转移至96孔磁力架上吸附3分钟", + "parameters": { + "source": "Orbital1", + "target": "P12" + } + }, + { + "step_number": 11, + "operation": "incubation", + "description": "吸附3分钟", + "parameters": { + "time": 180 + } + }, + { + "step_number": 12, + "operation": "transfer", + "description": "吸弃或倒弃废液", + "parameters": { + "source": "P12", + "target": "P22", + "tip_rack": "BC230", + "volume": 400 + } + }, + { + "step_number": 13, + "operation": "transfer", + "description": "重复加入75%乙醇", + "parameters": { + "source": "P3", + "target": "P12", + "tip_rack": "BC230", + "volume": 400 + } + }, + { + "step_number": 14, + "operation": "move_labware", + "description": "移动至振荡器进行振荡混匀", + "parameters": { + "source": "P12", + "target": "Orbital1" + } + }, + { + "step_number": 15, + "operation": "oscillation", + "description": "振荡混匀60秒", + "parameters": { + "rpm": 800, + "time": 60 + } + }, + { + "step_number": 16, + "operation": "move_labware", + "description": "转移至96孔磁力架上吸附3分钟", + "parameters": { + "source": "Orbital1", + "target": "P12" + } + }, + { + "step_number": 17, + "operation": "incubation", + "description": "吸附3分钟", + "parameters": { + "time": 180 + } + }, + { + "step_number": 18, + "operation": "transfer", + "description": "吸弃或倒弃废液", + "parameters": { + "source": "P12", + "target": "P22", + "tip_rack": "BC230", + "volume": 400 + } + }, + { + "step_number": 19, + "operation": "move_labware", + "description": "正放96孔板，空气干燥15分钟", + "parameters": { + "source": "P12", + "target": "P13" + } + }, + { + "step_number": 20, + "operation": "incubation", + "description": "空气干燥15分钟", + "parameters": { + "time": 900 + } + }, + { + "step_number": 21, + "operation": "transfer", + "description": "加入30-50μl Elution Buffer", + "parameters": { + "source": "P4", + "target": "P13", + "tip_rack": "BC230", + "volume": 40 + } + }, + { + "step_number": 22, + "operation": "move_labware", + "description": "移动至振荡器进行振荡混匀", + "parameters": { + "source": "P13", + "target": "Orbital1" + } + }, + { + "step_number": 23, + "operation": "oscillation", + "description": "振荡混匀60秒", + "parameters": { + "rpm": 800, + "time": 60 + } + }, + { + "step_number": 24, + "operation": "move_labware", + "description": "室温静置3分钟", + "parameters": { + "source": "Orbital1", + "target": "P13" + } + }, + { + "step_number": 25, + "operation": "incubation", + "description": "室温静置3分钟", + "parameters": { + "time": 180 + } + }, + { + "step_number": 26, + "operation": "move_labware", + "description": "转移至96孔磁力架上吸附2分钟", + "parameters": { + "source": "P13", + "target": "P12" + } + }, + { + "step_number": 27, + "operation": "incubation", + "description": "吸附2分钟", + "parameters": { + "time": 120 + } + }, + { + "step_number": 28, + "operation": "transfer", + "description": "将DNA转移至新的板中", + "parameters": { + "source": "P12", + "target": "P14", + "tip_rack": "BC230", + "volume": 40 + } } - ''' - - # 完整的labware配置信息（从biomek.py复制） - labware_with_liquid = ''' - [ - { - "id": "Tip Rack BC230 on TL1", - "parent": "deck", - "slot_on_deck": "TL1", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "Tip Rack BC230 on TL2", - "parent": "deck", - "slot_on_deck": "TL2", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "Tip Rack BC230 on TL3", - "parent": "deck", - "slot_on_deck": "TL3", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "Tip Rack BC230 on TL4", - "parent": "deck", - "slot_on_deck": "TL4", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "Tip Rack BC230 on TL5", - "parent": "deck", - "slot_on_deck": "TL5", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "Tip Rack BC230 on P5", - "parent": "deck", - "slot_on_deck": "P5", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "Tip Rack BC230 on P6", - "parent": "deck", - "slot_on_deck": "P6", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "Tip Rack BC230 on P15", - "parent": "deck", - "slot_on_deck": "P15", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "Tip Rack BC230 on P16", - "parent": "deck", - "slot_on_deck": "P16", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "stock plate on P1", - "parent": "deck", - "slot_on_deck": "P1", - "class_name": "nest_12_reservoir_15ml", - "liquid_type": [ - "master_mix" - ], - "liquid_volume": [10000], - "liquid_input_wells": [ - "A1" - ] - }, - { - "id": "stock plate on P2", - "parent": "deck", - "slot_on_deck": "P2", - "class_name": "nest_12_reservoir_15ml", - "liquid_type": [ - "bind beads" - ], - "liquid_volume": [10000], - "liquid_input_wells": [ - "A1" - ] - }, - { - "id": "stock plate on P3", - "parent": "deck", - "slot_on_deck": "P3", - "class_name": "nest_12_reservoir_15ml", - "liquid_type": [ - "ethyl alcohol" - ], - "liquid_volume": [10000], - "liquid_input_wells": [ - "A1" - ] - }, - { - "id": "elution buffer on P4", - "parent": "deck", - "slot_on_deck": "P4", - "class_name": "nest_12_reservoir_15ml", - "liquid_type": [ - "elution buffer" - ], - "liquid_volume": [5000], - "liquid_input_wells": [ - "A1" - ] - }, - { - "id": "oscillation", - "parent": "deck", - "slot_on_deck": "Orbital1", - "class_name": "Orbital", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "working plate on P11", - "parent": "deck", - "slot_on_deck": "P11", - "class_name": "NEST 2ml Deep Well Plate", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "magnetics module on P12", - "parent": "deck", - "slot_on_deck": "P12", - "class_name": "magnetics module", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "working plate on P13", - "parent": "deck", - "slot_on_deck": "P13", - "class_name": "NEST 2ml Deep Well Plate", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - }, - { - "id": "waste on P22", - "parent": "deck", - "slot_on_deck": "P22", - "class_name": "nest_1_reservoir_195ml", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [] - } - ] - ''' + ] +} + # 完整的labware配置信息 + labware_with_liquid = [ + { + "id": "Tip Rack BC230 TL1", + "parent": "deck", + "slot_on_deck": "TL1", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 TL2", + "parent": "deck", + "slot_on_deck": "TL2", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 TL3", + "parent": "deck", + "slot_on_deck": "TL3", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 TL4", + "parent": "deck", + "slot_on_deck": "TL4", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 TL5", + "parent": "deck", + "slot_on_deck": "TL5", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 P5", + "parent": "deck", + "slot_on_deck": "P5", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 P6", + "parent": "deck", + "slot_on_deck": "P6", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 P15", + "parent": "deck", + "slot_on_deck": "P15", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 P16", + "parent": "deck", + "slot_on_deck": "P16", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "stock plate on P1", + "parent": "deck", + "slot_on_deck": "P1", + "class_name": "AgilentReservoir", + "liquid_type": ["PCR product"], + "liquid_volume": [5000], + "liquid_input_wells": ["A1"] + }, + { + "id": "stock plate on P2", + "parent": "deck", + "slot_on_deck": "P2", + "class_name": "AgilentReservoir", + "liquid_type": ["bind beads"], + "liquid_volume": [100000], + "liquid_input_wells": ["A1"] + }, + { + "id": "stock plate on P3", + "parent": "deck", + "slot_on_deck": "P3", + "class_name": "AgilentReservoir", + "liquid_type": ["75% ethanol"], + "liquid_volume": [100000], + "liquid_input_wells": ["A1"] + }, + { + "id": "stock plate on P4", + "parent": "deck", + "slot_on_deck": "P4", + "class_name": "AgilentReservoir", + "liquid_type": ["Elution Buffer"], + "liquid_volume": [5000], + "liquid_input_wells": ["A1"] + }, + { + "id": "working plate on P11", + "parent": "deck", + "slot_on_deck": "P11", + "class_name": "BCDeep96Round", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "working plate on P12", + "parent": "deck", + "slot_on_deck": "P12", + "class_name": "BCDeep96Round", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "working plate on P13", + "parent": "deck", + "slot_on_deck": "P13", + "class_name": "BCDeep96Round", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "working plate on P14", + "parent": "deck", + "slot_on_deck": "P14", + "class_name": "BCDeep96Round", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "waste on P22", + "parent": "deck", + "slot_on_deck": "P22", + "class_name": "AgilentReservoir", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "oscillation", + "parent": "deck", + "slot_on_deck": "Orbital1", + "class_name": "Orbital", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + } +] # 创建handler实例 handler = LiquidHandlerBiomek()