From 6573c9e02eefdf63e563cd60323d247903146f4f Mon Sep 17 00:00:00 2001 From: qxw138 Date: Fri, 6 Jun 2025 22:42:06 +0800 Subject: [PATCH 1/3] biomek_test.py MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit biomek_test.py是最新的版本，运行它会生成complete_biomek_protocol.json --- unilabos/devices/liquid_handling/biomek.py | 146 +- .../devices/liquid_handling/biomek_test.py | 428 +- .../complete_biomek_protocol.json | 3760 +++++++++++++++++ 3 files changed, 4119 insertions(+), 215 deletions(-) create mode 100644 unilabos/devices/liquid_handling/complete_biomek_protocol.json diff --git a/unilabos/devices/liquid_handling/biomek.py b/unilabos/devices/liquid_handling/biomek.py index 173ea091..e4189e7c 100644 --- a/unilabos/devices/liquid_handling/biomek.py +++ b/unilabos/devices/liquid_handling/biomek.py @@ -688,133 +688,102 @@ if __name__ == "__main__": labware_with_liquid = ''' -[ { - "id": "stock plate on P1", - "parent": "deck", - "slot_on_deck": "P1", - "class_name": "nest_12_reservoir_15ml", - "liquid_type": [ - "master_mix" - ], - "liquid_volume": [10000], - "liquid_input_wells": [ - "A1" - ] - }, - { - "id": "Tip Rack BC230 TL2", - "parent": "deck", - "slot_on_deck": "TL2", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [ - ] - }, + [ { - "id": "Tip Rack BC230 TL2", + "id": "Tip Rack BC230 on TL1", + "parent": "deck", + "slot_on_deck": "TL1", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 on TL2", "parent": "deck", "slot_on_deck": "TL2", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on TL3", "parent": "deck", "slot_on_deck": "TL3", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on TL4", "parent": "deck", "slot_on_deck": "TL4", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on TL5", "parent": "deck", "slot_on_deck": "TL5", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on P5", "parent": "deck", "slot_on_deck": "P5", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on P6", "parent": "deck", "slot_on_deck": "P6", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] - }, - { - "id": "Tip Rack BC230 on P7", - "parent": "deck", - "slot_on_deck": "P7", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, { - "id": "Tip Rack BC230 on P8", + "id": "Tip Rack BC230 on P15", "parent": "deck", - "slot_on_deck": "P8", + "slot_on_deck": "P15", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { - "id": "Tip Rack BC230 P16", + { + "id": "Tip Rack BC230 on P16", "parent": "deck", "slot_on_deck": "P16", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, { - "id": "stock plate on 4", + "id": "stock plate on P1", "parent": "deck", - "slot_on_deck": "P2", + "slot_on_deck": "P1", "class_name": "nest_12_reservoir_15ml", "liquid_type": [ - "bind beads" + "master_mix" ], "liquid_volume": [10000], "liquid_input_wells": [ "A1" ] }, - { + { "id": "stock plate on P2", "parent": "deck", "slot_on_deck": "P2", @@ -827,7 +796,7 @@ labware_with_liquid = ''' "A1" ] }, - { + { "id": "stock plate on P3", "parent": "deck", "slot_on_deck": "P3", @@ -840,62 +809,65 @@ labware_with_liquid = ''' "A1" ] }, - - { + { + "id": "elution buffer on P4", + "parent": "deck", + "slot_on_deck": "P4", + "class_name": "nest_12_reservoir_15ml", + "liquid_type": [ + "elution buffer" + ], + "liquid_volume": [5000], + "liquid_input_wells": [ + "A1" + ] + }, + { "id": "oscillation", "parent": "deck", "slot_on_deck": "Orbital1", "class_name": "Orbital", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "working plate on P11", "parent": "deck", "slot_on_deck": "P11", "class_name": "NEST 2ml Deep Well Plate", - "liquid_type": [ - ], + "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "magnetics module on P12", "parent": "deck", "slot_on_deck": "P12", "class_name": "magnetics module", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "working plate on P13", "parent": "deck", "slot_on_deck": "P13", "class_name": "NEST 2ml Deep Well Plate", - "liquid_type": [ - ], + "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "waste on P22", "parent": "deck", "slot_on_deck": "P22", "class_name": "nest_1_reservoir_195ml", - "liquid_type": [ - ], + "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] } ] - ''' diff --git a/unilabos/devices/liquid_handling/biomek_test.py b/unilabos/devices/liquid_handling/biomek_test.py index e84000ab..85777604 100644 --- a/unilabos/devices/liquid_handling/biomek_test.py +++ b/unilabos/devices/liquid_handling/biomek_test.py @@ -13,6 +13,7 @@ from typing import List, Sequence, Optional, Union, Literal # from .liquid_handler_abstract import LiquidHandlerAbstract import json +import pathlib from typing import Sequence, Optional, List, Union, Literal @@ -33,6 +34,23 @@ class LiquidHandlerBiomek: self._status_queue = kwargs.get("status_queue", None) # 状态队列 self.temp_protocol = {} self.py32_path = "/opt/py32" # Biomek的Python 3.2路径 + + # 预定义的仪器分类 + self.tip_racks = [ + "BC230", "BC1025F", "BC50", "TipRack200", "TipRack1000", + "tip", "tips", "Tip", "Tips" + ] + + self.reservoirs = [ + "AgilentReservoir", "nest_12_reservoir_15ml", "nest_1_reservoir_195ml", + "reservoir", "Reservoir", "waste", "Waste" + ] + + self.plates_96 = [ + "BCDeep96Round", "Matrix96_750uL", "NEST 2ml Deep Well Plate", "nest_96_wellplate_100ul_pcr_full_skirt", + "nest_96_wellplate_200ul_flat", "Matrix96", "96", "plate", "Plate" + ] + self.aspirate_techniques = { 'MC P300 high':{ 'Position': 'P1', @@ -49,7 +67,7 @@ class LiquidHandlerBiomek: 'LocalPattern': True, 'Operation': 'Aspirate', 'OverrideHeight': False, - 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), + 'Pattern': (True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True, True), 'Prototype': 'MC P300 High', 'ReferencedPattern': '', 'RowsFirst': False, @@ -92,6 +110,30 @@ class LiquidHandlerBiomek: } + def _get_instrument_type(self, class_name: str) -> str: + """ + 根据class_name判断仪器类型 + + Returns: + str: "tip_rack", "reservoir", "plate_96", 或 "unknown" + """ + # 检查是否是枪头架 + for tip_name in self.tip_racks: + if tip_name in class_name: + return "tip_rack" + + # 检查是否是储液槽 + for reservoir_name in self.reservoirs: + if reservoir_name in class_name: + return "reservoir" + + # 检查是否是96孔板 + for plate_name in self.plates_96: + if plate_name in class_name: + return "plate_96" + + return "unknown" + def create_protocol( self, protocol_name: str, @@ -198,7 +240,104 @@ class LiquidHandlerBiomek: # } # self.temp_protocol["labwares"].append(resource) # return resource - + def instrument_setup_biomek( + self, + id: str, + parent: str, + slot_on_deck: str, + class_name: str, + liquid_type: list[str], + liquid_volume: list[int], + liquid_input_wells: list[str], + ): + """ + 设置Biomek仪器的参数配置，完全按照Biomek的实际格式 + + 根据不同的仪器类型（容器、tip rack等）设置相应的参数结构 + """ + + # 判断仪器类型 + instrument_type = self._get_instrument_type(class_name) + + config = {} # 初始化为空字典，以便在各种情况下都能返回 + + if instrument_type == "reservoir": + # 储液槽类型配置 + config = { + "Properties": { + "Name": id, # 使用id作为名称 + "Device": "", + "liquidtype": liquid_type[0] if liquid_type else "Water", + "BarCode": "", + "SenseEveryTime": False + }, + "Known": True, + "Class": f"LabwareClasses\\{class_name}", + "DataSets": {"Volume": {}}, + "RuntimeDataSets": {"Volume": {}}, + "EvalAmounts": (float(liquid_volume[0]),) if liquid_volume else (0.0,), + "Nominal": False, + "EvalLiquids": (liquid_type[0],) if liquid_type else ("Water",) + } + + elif instrument_type == "plate_96": + # 96孔板类型配置 + volume_per_well = float(liquid_volume[0]) if liquid_volume else 500.0 + liquid_per_well = liquid_type[0] if liquid_type else "Water" + + config = { + "Properties": { + "Name": id, # 使用id作为名称 + "Device": "", + "liquidtype": liquid_per_well, + "BarCode": "", + "SenseEveryTime": False + }, + "Known": True, + "Class": f"LabwareClasses\\{class_name}", + "DataSets": {"Volume": {}}, + "RuntimeDataSets": {"Volume": {}}, + "EvalAmounts": tuple([volume_per_well] * 96), + "Nominal": False, + "EvalLiquids": tuple([liquid_per_well] * 96) + } + + elif instrument_type == "tip_rack": + # 枪头架类型配置 + tip_config = { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": False, + "RT_Used": False, + "Dirty": False, + "RT_Dirty": False, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + } + + config = { + "Tips": tip_config, + "RT_Tips": tip_config.copy(), + "Properties": {}, + "Known": False, + "Class": f"LabwareClasses\\{class_name}", + "DataSets": {"Volume": {}}, + "RuntimeDataSets": {"Volume": {}} + } + + else: + # 未知类型或空位置的默认配置 + config = {} + + # 将配置直接添加到labwares列表中 + if config: # 只有非空配置才添加 + # 添加Name字段用于标识 + config["Name"] = id + config["Position"] = slot_on_deck + self.temp_protocol["labwares"].append(config) + + return def transfer_biomek( self, @@ -266,7 +405,8 @@ class LiquidHandlerBiomek: transfer_params["items"] = items transfer_params["Solvent"] = 'Water' transfer_params["TipLocation"] = tip_rack - self.temp_protocol["steps"].append(transfer_params) + tmp={'transfer': transfer_params} + self.temp_protocol["steps"].append(tmp) return @@ -287,7 +427,8 @@ class LiquidHandlerBiomek: "Target": target, "LeaveBottomLabware": False, } - self.temp_protocol["steps"].append(move_params) + tmp={'move': move_params} + self.temp_protocol["steps"].append(tmp) return @@ -304,7 +445,8 @@ class LiquidHandlerBiomek: "Time": time, "Mode": "TimedResource" } - self.temp_protocol["steps"].append(incubation_params) + tmp={'incubation': incubation_params} + self.temp_protocol["steps"].append(tmp) return @@ -321,14 +463,19 @@ class LiquidHandlerBiomek: 'Parameters': (str(rpm), '2', str(time), 'CounterClockwise'), 'Command': 'Timed Shake' } - self.temp_protocol["steps"].append(oscillation_params) + tmp={'oscillation': oscillation_params} + self.temp_protocol["steps"].append(tmp) return if __name__ == "__main__": - + + print("=== Biomek完整流程测试 ===") + print("包含: 仪器设置 + 完整实验步骤") + + # 完整的步骤信息（从biomek.py复制） steps_info = ''' { "steps": [ @@ -592,136 +739,104 @@ if __name__ == "__main__": } ''' - - -labware_with_liquid = ''' -[ { - "id": "stock plate on P1", - "parent": "deck", - "slot_on_deck": "P1", - "class_name": "nest_12_reservoir_15ml", - "liquid_type": [ - "master_mix" - ], - "liquid_volume": [10000], - "liquid_input_wells": [ - "A1" - ] - }, - { - "id": "Tip Rack BC230 TL2", - "parent": "deck", - "slot_on_deck": "TL2", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [ - ] - }, + # 完整的labware配置信息（从biomek.py复制） + labware_with_liquid = ''' + [ { - "id": "Tip Rack BC230 TL2", + "id": "Tip Rack BC230 on TL1", + "parent": "deck", + "slot_on_deck": "TL1", + "class_name": "BC230", + "liquid_type": [], + "liquid_volume": [], + "liquid_input_wells": [] + }, + { + "id": "Tip Rack BC230 on TL2", "parent": "deck", "slot_on_deck": "TL2", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on TL3", "parent": "deck", "slot_on_deck": "TL3", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on TL4", "parent": "deck", "slot_on_deck": "TL4", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on TL5", "parent": "deck", "slot_on_deck": "TL5", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on P5", "parent": "deck", "slot_on_deck": "P5", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "Tip Rack BC230 on P6", "parent": "deck", "slot_on_deck": "P6", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] - }, - { - "id": "Tip Rack BC230 on P7", - "parent": "deck", - "slot_on_deck": "P7", - "class_name": "BC230", - "liquid_type": [], - "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, { - "id": "Tip Rack BC230 on P8", + "id": "Tip Rack BC230 on P15", "parent": "deck", - "slot_on_deck": "P8", + "slot_on_deck": "P15", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { - "id": "Tip Rack BC230 P16", + { + "id": "Tip Rack BC230 on P16", "parent": "deck", "slot_on_deck": "P16", "class_name": "BC230", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, { - "id": "stock plate on 4", + "id": "stock plate on P1", "parent": "deck", - "slot_on_deck": "P2", + "slot_on_deck": "P1", "class_name": "nest_12_reservoir_15ml", "liquid_type": [ - "bind beads" + "master_mix" ], "liquid_volume": [10000], "liquid_input_wells": [ "A1" ] }, - { + { "id": "stock plate on P2", "parent": "deck", "slot_on_deck": "P2", @@ -734,7 +849,7 @@ labware_with_liquid = ''' "A1" ] }, - { + { "id": "stock plate on P3", "parent": "deck", "slot_on_deck": "P3", @@ -747,95 +862,152 @@ labware_with_liquid = ''' "A1" ] }, - - { + { + "id": "elution buffer on P4", + "parent": "deck", + "slot_on_deck": "P4", + "class_name": "nest_12_reservoir_15ml", + "liquid_type": [ + "elution buffer" + ], + "liquid_volume": [5000], + "liquid_input_wells": [ + "A1" + ] + }, + { "id": "oscillation", "parent": "deck", "slot_on_deck": "Orbital1", "class_name": "Orbital", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "working plate on P11", "parent": "deck", "slot_on_deck": "P11", "class_name": "NEST 2ml Deep Well Plate", - "liquid_type": [ - ], + "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "magnetics module on P12", "parent": "deck", "slot_on_deck": "P12", "class_name": "magnetics module", "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "working plate on P13", "parent": "deck", "slot_on_deck": "P13", "class_name": "NEST 2ml Deep Well Plate", - "liquid_type": [ - ], + "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] }, - { + { "id": "waste on P22", "parent": "deck", "slot_on_deck": "P22", "class_name": "nest_1_reservoir_195ml", - "liquid_type": [ - ], + "liquid_type": [], "liquid_volume": [], - "liquid_input_wells": [ - ] + "liquid_input_wells": [] } ] - ''' + # 创建handler实例 + handler = LiquidHandlerBiomek() + + # 创建协议 + protocol = handler.create_protocol( + protocol_name="DNA纯化完整流程", + protocol_description="使用磁珠进行DNA纯化的完整自动化流程", + protocol_version="1.0", + protocol_author="Biomek系统", + protocol_date="2024-01-01", + protocol_type="DNA_purification" + ) + print("\n=== 第一步：设置所有仪器 ===") + # 解析labware配置 + labwares = json.loads(labware_with_liquid) + + # 设置所有仪器 + instrument_count = 0 + for labware in labwares: + print(f"设置仪器: {labware['id']} ({labware['class_name']}) 在位置 {labware['slot_on_deck']}") + handler.instrument_setup_biomek( + id=labware['id'], + parent=labware['parent'], + slot_on_deck=labware['slot_on_deck'], + class_name=labware['class_name'], + liquid_type=labware['liquid_type'], + liquid_volume=labware['liquid_volume'], + liquid_input_wells=labware['liquid_input_wells'] + ) + instrument_count += 1 + + print(f"总共设置了 {instrument_count} 个仪器位置") -handler = LiquidHandlerBiomek() + print("\n=== 第二步：执行实验步骤 ===") + # 解析步骤信息 + input_steps = json.loads(steps_info) + + # 执行所有步骤 + step_count = 0 + for step in input_steps['steps']: + operation = step['operation'] + parameters = step['parameters'] + description = step['description'] + + print(f"步骤 {step['step_number']}: {description}") + + if operation == 'transfer': + handler.transfer_biomek( + source=parameters['source'], + target=parameters['target'], + volume=parameters['volume'], + tip_rack=parameters['tip_rack'], + aspirate_techniques='MC P300 high', + dispense_techniques='MC P300 high' + ) + elif operation == 'move_labware': + handler.move_biomek( + source=parameters['source'], + target=parameters['target'] + ) + elif operation == 'oscillation': + handler.oscillation_biomek( + rpm=parameters['rpm'], + time=parameters['time'] + ) + elif operation == 'incubation': + handler.incubation_biomek( + time=parameters['time'] + ) + + step_count += 1 + + print(f"总共执行了 {step_count} 个步骤") -handler.temp_protocol = { - "meta": {}, - "labwares": [], - "steps": [] -} - -input_steps = json.loads(steps_info) -labwares = json.loads(labware_with_liquid) - -for step in input_steps['steps']: - operation = step['operation'] - parameters = step['parameters'] - - if operation == 'transfer': - handler.transfer_biomek(source=parameters['source'], - target=parameters['target'], - volume=parameters['volume'], - tip_rack=parameters['tip_rack'], - aspirate_techniques='MC P300 high', - dispense_techniques='MC P300 high') - elif operation == 'move_labware': - handler.move_biomek(source=parameters['source'], - target=parameters['target']) - elif operation == 'oscillation': - handler.oscillation_biomek(rpm=parameters['rpm'], - time=parameters['time']) - elif operation == 'incubation': - handler.incubation_biomek(time=parameters['time']) - -print(json.dumps(handler.temp_protocol, indent=4)) + print("\n=== 第三步：保存完整协议 ===") + # 获取脚本目录 + script_dir = pathlib.Path(__file__).parent + + # 保存完整协议 + complete_output_path = script_dir / "complete_biomek_protocol.json" + with open(complete_output_path, 'w', encoding='utf-8') as f: + json.dump(handler.temp_protocol, f, indent=4, ensure_ascii=False) + + print(f"完整协议已保存到: {complete_output_path}") + + print("\n=== 测试完成 ===") + print("完整的DNA纯化流程已成功转换为Biomek格式！") diff --git a/unilabos/devices/liquid_handling/complete_biomek_protocol.json b/unilabos/devices/liquid_handling/complete_biomek_protocol.json new file mode 100644 index 00000000..2c0e95fd --- /dev/null +++ b/unilabos/devices/liquid_handling/complete_biomek_protocol.json @@ -0,0 +1,3760 @@ +{ + "meta": { + "name": "DNA纯化完整流程", + "description": "使用磁珠进行DNA纯化的完整自动化流程", + "version": "1.0", + "author": "Biomek系统", + "date": "2024-01-01", + "type": "DNA_purification" + }, + "labwares": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on TL1", + "Position": "TL1" + }, + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on TL2", + "Position": "TL2" + }, + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on TL3", + "Position": "TL3" + }, + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on TL4", + "Position": "TL4" + }, + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on TL5", + "Position": "TL5" + }, + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on P5", + "Position": "P5" + }, + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on P6", + "Position": "P6" + }, + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on P15", + "Position": "P15" + }, + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "Name": "Tip Rack BC230 on P16", + "Position": "P16" + }, + { + "Properties": { + "Name": "stock plate on P1", + "Device": "", + "liquidtype": "master_mix", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_12_reservoir_15ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 10000.0 + ], + "Nominal": false, + "EvalLiquids": [ + "master_mix" + ], + "Name": "stock plate on P1", + "Position": "P1" + }, + { + "Properties": { + "Name": "stock plate on P2", + "Device": "", + "liquidtype": "bind beads", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_12_reservoir_15ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 10000.0 + ], + "Nominal": false, + "EvalLiquids": [ + "bind beads" + ], + "Name": "stock plate on P2", + "Position": "P2" + }, + { + "Properties": { + "Name": "stock plate on P3", + "Device": "", + "liquidtype": "ethyl alcohol", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_12_reservoir_15ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 10000.0 + ], + "Nominal": false, + "EvalLiquids": [ + "ethyl alcohol" + ], + "Name": "stock plate on P3", + "Position": "P3" + }, + { + "Properties": { + "Name": "elution buffer on P4", + "Device": "", + "liquidtype": "elution buffer", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_12_reservoir_15ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 5000.0 + ], + "Nominal": false, + "EvalLiquids": [ + "elution buffer" + ], + "Name": "elution buffer on P4", + "Position": "P4" + }, + { + "Properties": { + "Name": "working plate on P11", + "Device": "", + "liquidtype": "Water", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\NEST 2ml Deep Well Plate", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0 + ], + "Nominal": false, + "EvalLiquids": [ + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water" + ], + "Name": "working plate on P11", + "Position": "P11" + }, + { + "Properties": { + "Name": "working plate on P13", + "Device": "", + "liquidtype": "Water", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\NEST 2ml Deep Well Plate", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0 + ], + "Nominal": false, + "EvalLiquids": [ + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water" + ], + "Name": "working plate on P13", + "Position": "P13" + }, + { + "Properties": { + "Name": "waste on P22", + "Device": "", + "liquidtype": "Water", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_1_reservoir_195ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 0.0 + ], + "Nominal": false, + "EvalLiquids": [ + "Water" + ], + "Name": "waste on P22", + "Position": "P22" + } + ], + "steps": [ + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P11", + "Target": "Orbital1", + "LeaveBottomLabware": false + } + }, + { + "oscillation": { + "Device": "OrbitalShaker0", + "Parameters": [ + "800", + "2", + "300", + "CounterClockwise" + ], + "Command": "Timed Shake" + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "Orbital1", + "Target": "P11", + "LeaveBottomLabware": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P11", + "Target": "P12", + "LeaveBottomLabware": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 180, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P12", + "Target": "Orbital1", + "LeaveBottomLabware": false + } + }, + { + "oscillation": { + "Device": "OrbitalShaker0", + "Parameters": [ + "800", + "2", + "45", + "CounterClockwise" + ], + "Command": "Timed Shake" + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "Orbital1", + "Target": "P12", + "LeaveBottomLabware": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 180, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P12", + "Target": "Orbital1", + "LeaveBottomLabware": false + } + }, + { + "oscillation": { + "Device": "OrbitalShaker0", + "Parameters": [ + "800", + "2", + "45", + "CounterClockwise" + ], + "Command": "Timed Shake" + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "Orbital1", + "Target": "P12", + "LeaveBottomLabware": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 180, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 900, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P12", + "Target": "Orbital1", + "LeaveBottomLabware": false + } + }, + { + "oscillation": { + "Device": "OrbitalShaker0", + "Parameters": [ + "800", + "2", + "60", + "CounterClockwise" + ], + "Command": "Timed Shake" + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "Orbital1", + "Target": "P12", + "LeaveBottomLabware": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 180, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + } + ] +} \ No newline at end of file From e840516ba4df2d09e926b6cffe077058e23b9930 Mon Sep 17 00:00:00 2001 From: qxw138 Date: Fri, 6 Jun 2025 22:50:11 +0800 Subject: [PATCH 2/3] Update biomek.py --- unilabos/devices/liquid_handling/biomek.py | 52 +++++++++++----------- 1 file changed, 26 insertions(+), 26 deletions(-) diff --git a/unilabos/devices/liquid_handling/biomek.py b/unilabos/devices/liquid_handling/biomek.py index 925ac53c..7d671a68 100644 --- a/unilabos/devices/liquid_handling/biomek.py +++ b/unilabos/devices/liquid_handling/biomek.py @@ -873,35 +873,35 @@ labware_with_liquid = ''' handler = LiquidHandlerBiomek() - handler.temp_protocol = { - "meta": {}, - "labwares": [], - "steps": [] - } +handler.temp_protocol = { + "meta": {}, + "labwares": [], + "steps": [] +} - input_steps = json.loads(steps_info) - labwares = json.loads(labware_with_liquid) +input_steps = json.loads(steps_info) +labwares = json.loads(labware_with_liquid) - for step in input_steps['steps']: - operation = step['operation'] - parameters = step['parameters'] +for step in input_steps['steps']: + operation = step['operation'] + parameters = step['parameters'] - if operation == 'transfer': - handler.transfer_biomek(source=parameters['source'], - target=parameters['target'], - volume=parameters['volume'], - tip_rack=parameters['tip_rack'], - aspirate_techniques='MC P300 high', - dispense_techniques='MC P300 high') - elif operation == 'move_labware': - handler.move_biomek(source=parameters['source'], - target=parameters['target']) - elif operation == 'oscillation': - handler.oscillation_biomek(rpm=parameters['rpm'], - time=parameters['time']) - elif operation == 'incubation': - handler.incubation_biomek(time=parameters['time']) + if operation == 'transfer': + handler.transfer_biomek(source=parameters['source'], + target=parameters['target'], + volume=parameters['volume'], + tip_rack=parameters['tip_rack'], + aspirate_techniques='MC P300 high', + dispense_techniques='MC P300 high') + elif operation == 'move_labware': + handler.move_biomek(source=parameters['source'], + target=parameters['target']) + elif operation == 'oscillation': + handler.oscillation_biomek(rpm=parameters['rpm'], + time=parameters['time']) + elif operation == 'incubation': + handler.incubation_biomek(time=parameters['time']) - print(json.dumps(handler.temp_protocol, indent=4)) +print(json.dumps(handler.temp_protocol, indent=4)) From 3f53f88390c3fe3d499f4534e52e56d6a81ab10e Mon Sep 17 00:00:00 2001 From: qxw138 Date: Sat, 7 Jun 2025 15:21:20 +0800 Subject: [PATCH 3/3] biomek_test.py --- .../devices/liquid_handling/biomek_test.py | 25 +- .../complete_biomek_protocol_0607.json | 3762 +++++++++++++++++ 2 files changed, 3773 insertions(+), 14 deletions(-) create mode 100644 unilabos/devices/liquid_handling/complete_biomek_protocol_0607.json diff --git a/unilabos/devices/liquid_handling/biomek_test.py b/unilabos/devices/liquid_handling/biomek_test.py index 85777604..185d47b5 100644 --- a/unilabos/devices/liquid_handling/biomek_test.py +++ b/unilabos/devices/liquid_handling/biomek_test.py @@ -168,7 +168,7 @@ class LiquidHandlerBiomek: "date": protocol_date, "type": protocol_type, }, - "labwares": [], + "labwares": {}, # 改为字典格式以匹配DeckItems "steps": [], } return self.temp_protocol @@ -251,15 +251,16 @@ class LiquidHandlerBiomek: liquid_input_wells: list[str], ): """ - 设置Biomek仪器的参数配置，完全按照Biomek的实际格式 + 设置Biomek仪器的参数配置，按照DeckItems格式 根据不同的仪器类型（容器、tip rack等）设置相应的参数结构 + 位置作为键，配置列表作为值 """ # 判断仪器类型 instrument_type = self._get_instrument_type(class_name) - config = {} # 初始化为空字典，以便在各种情况下都能返回 + config = None # 初始化为None if instrument_type == "reservoir": # 储液槽类型配置 @@ -325,17 +326,13 @@ class LiquidHandlerBiomek: "DataSets": {"Volume": {}}, "RuntimeDataSets": {"Volume": {}} } - - else: - # 未知类型或空位置的默认配置 - config = {} - # 将配置直接添加到labwares列表中 - if config: # 只有非空配置才添加 - # 添加Name字段用于标识 - config["Name"] = id - config["Position"] = slot_on_deck - self.temp_protocol["labwares"].append(config) + # 按照DeckItems格式存储：位置作为键，配置列表作为值 + if config is not None: + self.temp_protocol["labwares"][slot_on_deck] = [config] + else: + # 空位置 + self.temp_protocol["labwares"][slot_on_deck] = [] return @@ -1003,7 +1000,7 @@ if __name__ == "__main__": script_dir = pathlib.Path(__file__).parent # 保存完整协议 - complete_output_path = script_dir / "complete_biomek_protocol.json" + complete_output_path = script_dir / "complete_biomek_protocol_0607.json" with open(complete_output_path, 'w', encoding='utf-8') as f: json.dump(handler.temp_protocol, f, indent=4, ensure_ascii=False) diff --git a/unilabos/devices/liquid_handling/complete_biomek_protocol_0607.json b/unilabos/devices/liquid_handling/complete_biomek_protocol_0607.json new file mode 100644 index 00000000..be73033c --- /dev/null +++ b/unilabos/devices/liquid_handling/complete_biomek_protocol_0607.json @@ -0,0 +1,3762 @@ +{ + "meta": { + "name": "DNA纯化完整流程", + "description": "使用磁珠进行DNA纯化的完整自动化流程", + "version": "1.0", + "author": "Biomek系统", + "date": "2024-01-01", + "type": "DNA_purification" + }, + "labwares": { + "TL1": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "TL2": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "TL3": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "TL4": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "TL5": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "P5": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "P6": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "P15": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "P16": [ + { + "Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "RT_Tips": { + "Class": "TipClasses\\T230", + "Contents": [], + "_RT_Contents": [], + "Used": false, + "RT_Used": false, + "Dirty": false, + "RT_Dirty": false, + "MaxVolumeUsed": 0.0, + "RT_MaxVolumeUsed": 0.0 + }, + "Properties": {}, + "Known": false, + "Class": "LabwareClasses\\BC230", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + } + } + ], + "P1": [ + { + "Properties": { + "Name": "stock plate on P1", + "Device": "", + "liquidtype": "master_mix", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_12_reservoir_15ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 10000.0 + ], + "Nominal": false, + "EvalLiquids": [ + "master_mix" + ] + } + ], + "P2": [ + { + "Properties": { + "Name": "stock plate on P2", + "Device": "", + "liquidtype": "bind beads", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_12_reservoir_15ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 10000.0 + ], + "Nominal": false, + "EvalLiquids": [ + "bind beads" + ] + } + ], + "P3": [ + { + "Properties": { + "Name": "stock plate on P3", + "Device": "", + "liquidtype": "ethyl alcohol", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_12_reservoir_15ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 10000.0 + ], + "Nominal": false, + "EvalLiquids": [ + "ethyl alcohol" + ] + } + ], + "P4": [ + { + "Properties": { + "Name": "elution buffer on P4", + "Device": "", + "liquidtype": "elution buffer", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_12_reservoir_15ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 5000.0 + ], + "Nominal": false, + "EvalLiquids": [ + "elution buffer" + ] + } + ], + "Orbital1": [], + "P11": [ + { + "Properties": { + "Name": "working plate on P11", + "Device": "", + "liquidtype": "Water", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\NEST 2ml Deep Well Plate", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0 + ], + "Nominal": false, + "EvalLiquids": [ + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water" + ] + } + ], + "P12": [], + "P13": [ + { + "Properties": { + "Name": "working plate on P13", + "Device": "", + "liquidtype": "Water", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\NEST 2ml Deep Well Plate", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0, + 500.0 + ], + "Nominal": false, + "EvalLiquids": [ + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water", + "Water" + ] + } + ], + "P22": [ + { + "Properties": { + "Name": "waste on P22", + "Device": "", + "liquidtype": "Water", + "BarCode": "", + "SenseEveryTime": false + }, + "Known": true, + "Class": "LabwareClasses\\nest_1_reservoir_195ml", + "DataSets": { + "Volume": {} + }, + "RuntimeDataSets": { + "Volume": {} + }, + "EvalAmounts": [ + 0.0 + ], + "Nominal": false, + "EvalLiquids": [ + "Water" + ] + } + ] + }, + "steps": [ + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P11", + "Target": "Orbital1", + "LeaveBottomLabware": false + } + }, + { + "oscillation": { + "Device": "OrbitalShaker0", + "Parameters": [ + "800", + "2", + "300", + "CounterClockwise" + ], + "Command": "Timed Shake" + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "Orbital1", + "Target": "P11", + "LeaveBottomLabware": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P11", + "Target": "P12", + "LeaveBottomLabware": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 180, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P12", + "Target": "Orbital1", + "LeaveBottomLabware": false + } + }, + { + "oscillation": { + "Device": "OrbitalShaker0", + "Parameters": [ + "800", + "2", + "45", + "CounterClockwise" + ], + "Command": "Timed Shake" + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "Orbital1", + "Target": "P12", + "LeaveBottomLabware": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 180, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P12", + "Target": "Orbital1", + "LeaveBottomLabware": false + } + }, + { + "oscillation": { + "Device": "OrbitalShaker0", + "Parameters": [ + "800", + "2", + "45", + "CounterClockwise" + ], + "Command": "Timed Shake" + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "Orbital1", + "Target": "P12", + "LeaveBottomLabware": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 180, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 900, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "P12", + "Target": "Orbital1", + "LeaveBottomLabware": false + } + }, + { + "oscillation": { + "Device": "OrbitalShaker0", + "Parameters": [ + "800", + "2", + "60", + "CounterClockwise" + ], + "Command": "Timed Shake" + } + }, + { + "move": { + "Pod": "Pod1", + "GripSide": "A1 near", + "Source": "Orbital1", + "Target": "P12", + "LeaveBottomLabware": false + } + }, + { + "incubation": { + "Message": "Paused", + "Location": "the whole system", + "Time": 180, + "Mode": "TimedResource" + } + }, + { + "transfer": { + "Span8": false, + "Pod": "Pod1", + "items": [ + { + "Position": "P12", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Well Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Aspirate", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": true, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + }, + { + "Position": "P13", + "Height": -2.0, + "Volume": "40", + "liquidtype": "Tip Contents", + "WellsX": 12, + "LabwareClass": "Matrix96_750uL", + "AutoSelectPrototype": true, + "ColsFirst": true, + "CustomHeight": false, + "DataSetPattern": false, + "HeightFrom": 0, + "LocalPattern": true, + "Operation": "Dispense", + "OverrideHeight": false, + "Pattern": [ + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true, + true + ], + "Prototype": "MC P300 High", + "ReferencedPattern": "", + "RowsFirst": false, + "SectionExpression": "", + "SelectionInfo": [ + 1 + ], + "SetMark": true, + "Source": false, + "StartAtMark": false, + "StartAtSelection": true, + "UseExpression": false + } + ], + "Wash": false, + "Dynamic?": true, + "AutoSelectActiveWashTechnique": false, + "ActiveWashTechnique": "", + "ChangeTipsBetweenDests": true, + "ChangeTipsBetweenSources": false, + "DefaultCaption": "", + "UseExpression": false, + "LeaveTipsOn": false, + "MandrelExpression": "", + "Repeats": "1", + "RepeatsByVolume": false, + "Replicates": "1", + "ShowTipHandlingDetails": false, + "ShowTransferDetails": true, + "Solvent": "Water", + "Span8Wash": false, + "Span8WashVolume": "2", + "Span8WasteVolume": "1", + "SplitVolume": false, + "SplitVolumeCleaning": false, + "Stop": "Destinations", + "TipLocation": "BC230", + "UseCurrentTips": false, + "UseDisposableTips": false, + "UseFixedTips": false, + "UseJIT": true, + "UseMandrelSelection": true, + "UseProbes": [ + true, + true, + true, + true, + true, + true, + true, + true + ], + "WashCycles": "4", + "WashVolume": "110%", + "Wizard": false + } + } + ] +} \ No newline at end of file