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4 Commits
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c7c14d2332
| Author | SHA1 | Date | |
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c7c14d2332 | ||
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6fdd482649 | ||
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d390236318 | ||
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ed8ee29732 |
@@ -218,7 +218,7 @@ def main():
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if hasattr(BasicConfig, "log_level"):
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logger.info(f"Log level set to '{BasicConfig.log_level}' from config file.")
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configure_logger(loglevel=BasicConfig.log_level)
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configure_logger(loglevel=BasicConfig.log_level, working_dir=working_dir)
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if args_dict["addr"] == "test":
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print_status("使用测试环境地址", "info")
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@@ -147,6 +147,9 @@ class LiquidHandlerMiddleware(LiquidHandler):
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offsets: Optional[List[Coordinate]] = None,
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**backend_kwargs,
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):
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# 如果 use_channels 为 None,使用默认值(所有通道)
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if use_channels is None:
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use_channels = list(range(self.channel_num))
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if not offsets or (isinstance(offsets, list) and len(offsets) != len(use_channels)):
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offsets = [Coordinate.zero()] * len(use_channels)
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if self._simulator:
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@@ -759,7 +762,7 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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blow_out_air_volume=current_dis_blow_out_air_volume,
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spread=spread,
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)
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if delays is not None:
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if delays is not None and len(delays) > 1:
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await self.custom_delay(seconds=delays[1])
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await self.touch_tip(current_targets)
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await self.discard_tips()
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@@ -833,17 +836,19 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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spread=spread,
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)
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if delays is not None:
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if delays is not None and len(delays) > 1:
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await self.custom_delay(seconds=delays[1])
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await self.mix(
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targets=[targets[_]],
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mix_time=mix_time,
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mix_vol=mix_vol,
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offsets=offsets if offsets else None,
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height_to_bottom=mix_liquid_height if mix_liquid_height else None,
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mix_rate=mix_rate if mix_rate else None,
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)
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if delays is not None:
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# 只有在 mix_time 有效时才调用 mix
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if mix_time is not None and mix_time > 0:
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await self.mix(
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targets=[targets[_]],
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mix_time=mix_time,
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mix_vol=mix_vol,
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offsets=offsets if offsets else None,
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height_to_bottom=mix_liquid_height if mix_liquid_height else None,
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mix_rate=mix_rate if mix_rate else None,
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)
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if delays is not None and len(delays) > 1:
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await self.custom_delay(seconds=delays[1])
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await self.touch_tip(targets[_])
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await self.discard_tips()
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@@ -893,18 +898,20 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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blow_out_air_volume=current_dis_blow_out_air_volume,
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spread=spread,
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)
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if delays is not None:
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if delays is not None and len(delays) > 1:
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await self.custom_delay(seconds=delays[1])
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await self.mix(
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targets=current_targets,
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mix_time=mix_time,
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mix_vol=mix_vol,
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offsets=offsets if offsets else None,
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height_to_bottom=mix_liquid_height if mix_liquid_height else None,
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mix_rate=mix_rate if mix_rate else None,
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)
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if delays is not None:
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# 只有在 mix_time 有效时才调用 mix
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if mix_time is not None and mix_time > 0:
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await self.mix(
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targets=current_targets,
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mix_time=mix_time,
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mix_vol=mix_vol,
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offsets=offsets if offsets else None,
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height_to_bottom=mix_liquid_height if mix_liquid_height else None,
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mix_rate=mix_rate if mix_rate else None,
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)
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if delays is not None and len(delays) > 1:
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await self.custom_delay(seconds=delays[1])
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await self.touch_tip(current_targets)
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await self.discard_tips()
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@@ -942,60 +949,146 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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delays: Optional[List[int]] = None,
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none_keys: List[str] = [],
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):
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"""Transfer liquid from each *source* well/plate to the corresponding *target*.
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"""Transfer liquid with automatic mode detection.
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Supports three transfer modes:
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1. One-to-many (1 source -> N targets): Distribute from one source to multiple targets
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2. One-to-one (N sources -> N targets): Standard transfer, each source to corresponding target
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3. Many-to-one (N sources -> 1 target): Combine multiple sources into one target
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Parameters
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----------
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asp_vols, dis_vols
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Single volume (µL) or list matching the number of transfers.
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Single volume (µL) or list. Automatically expanded based on transfer mode.
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sources, targets
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Same‑length sequences of containers (wells or plates). In 96‑well mode
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each must contain exactly one plate.
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Containers (wells or plates). Length determines transfer mode:
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- len(sources) == 1, len(targets) > 1: One-to-many mode
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- len(sources) == len(targets): One-to-one mode
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- len(sources) > 1, len(targets) == 1: Many-to-one mode
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tip_racks
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One or more TipRacks providing fresh tips.
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is_96_well
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Set *True* to use the 96‑channel head.
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"""
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# 确保 use_channels 有默认值
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if use_channels is None:
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use_channels = [0] if self.channel_num >= 1 else list(range(self.channel_num))
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if is_96_well:
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pass # This mode is not verified.
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else:
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if len(asp_vols) != len(targets):
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raise ValueError(f"Length of `asp_vols` {len(asp_vols)} must match `targets` {len(targets)}.")
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# 转换体积参数为列表
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if isinstance(asp_vols, (int, float)):
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asp_vols = [float(asp_vols)]
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else:
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asp_vols = [float(v) for v in asp_vols]
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if isinstance(dis_vols, (int, float)):
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dis_vols = [float(dis_vols)]
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else:
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dis_vols = [float(v) for v in dis_vols]
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# 识别传输模式
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num_sources = len(sources)
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num_targets = len(targets)
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if num_sources == 1 and num_targets > 1:
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# 模式1: 一对多 (1 source -> N targets)
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await self._transfer_one_to_many(
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sources[0], targets, tip_racks, use_channels,
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asp_vols, dis_vols, asp_flow_rates, dis_flow_rates,
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offsets, touch_tip, liquid_height, blow_out_air_volume,
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spread, mix_stage, mix_times, mix_vol, mix_rate,
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mix_liquid_height, delays
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)
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elif num_sources > 1 and num_targets == 1:
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# 模式2: 多对一 (N sources -> 1 target)
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await self._transfer_many_to_one(
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sources, targets[0], tip_racks, use_channels,
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asp_vols, dis_vols, asp_flow_rates, dis_flow_rates,
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offsets, touch_tip, liquid_height, blow_out_air_volume,
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spread, mix_stage, mix_times, mix_vol, mix_rate,
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mix_liquid_height, delays
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)
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elif num_sources == num_targets:
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# 模式3: 一对一 (N sources -> N targets) - 原有逻辑
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await self._transfer_one_to_one(
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sources, targets, tip_racks, use_channels,
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asp_vols, dis_vols, asp_flow_rates, dis_flow_rates,
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offsets, touch_tip, liquid_height, blow_out_air_volume,
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spread, mix_stage, mix_times, mix_vol, mix_rate,
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mix_liquid_height, delays
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)
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else:
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raise ValueError(
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f"Unsupported transfer mode: {num_sources} sources -> {num_targets} targets. "
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"Supported modes: 1->N, N->1, or N->N."
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)
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# 首先应该对任务分组,然后每次1个/8个进行操作处理
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if len(use_channels) == 1:
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for _ in range(len(targets)):
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tip = []
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for ___ in range(len(use_channels)):
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tip.extend(next(self.current_tip))
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await self.pick_up_tips(tip)
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async def _transfer_one_to_one(
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self,
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sources: Sequence[Container],
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targets: Sequence[Container],
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tip_racks: Sequence[TipRack],
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use_channels: List[int],
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asp_vols: List[float],
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dis_vols: List[float],
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asp_flow_rates: Optional[List[Optional[float]]],
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dis_flow_rates: Optional[List[Optional[float]]],
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offsets: Optional[List[Coordinate]],
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touch_tip: bool,
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liquid_height: Optional[List[Optional[float]]],
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blow_out_air_volume: Optional[List[Optional[float]]],
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spread: Literal["wide", "tight", "custom"],
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mix_stage: Optional[Literal["none", "before", "after", "both"]],
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mix_times: Optional[int],
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mix_vol: Optional[int],
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mix_rate: Optional[int],
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mix_liquid_height: Optional[float],
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delays: Optional[List[int]],
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):
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"""一对一传输模式:N sources -> N targets"""
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# 验证参数长度
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if len(asp_vols) != len(targets):
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raise ValueError(f"Length of `asp_vols` {len(asp_vols)} must match `targets` {len(targets)}.")
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if len(dis_vols) != len(targets):
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raise ValueError(f"Length of `dis_vols` {len(dis_vols)} must match `targets` {len(targets)}.")
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if len(sources) != len(targets):
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raise ValueError(f"Length of `sources` {len(sources)} must match `targets` {len(targets)}.")
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await self.aspirate(
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resources=[sources[_]],
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vols=[asp_vols[_]],
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use_channels=use_channels,
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flow_rates=[asp_flow_rates[0]] if asp_flow_rates else None,
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offsets=[offsets[0]] if offsets else None,
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liquid_height=[liquid_height[0]] if liquid_height else None,
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blow_out_air_volume=[blow_out_air_volume[0]] if blow_out_air_volume else None,
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spread=spread,
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)
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if delays is not None:
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await self.custom_delay(seconds=delays[0])
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await self.dispense(
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resources=[targets[_]],
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vols=[dis_vols[_]],
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use_channels=use_channels,
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flow_rates=[dis_flow_rates[1]] if dis_flow_rates else None,
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offsets=[offsets[1]] if offsets else None,
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blow_out_air_volume=[blow_out_air_volume[1]] if blow_out_air_volume else None,
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liquid_height=[liquid_height[1]] if liquid_height else None,
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spread=spread,
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)
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if delays is not None:
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await self.custom_delay(seconds=delays[1])
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if len(use_channels) == 1:
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for _ in range(len(targets)):
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tip = []
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for ___ in range(len(use_channels)):
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tip.extend(next(self.current_tip))
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await self.pick_up_tips(tip)
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await self.aspirate(
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resources=[sources[_]],
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vols=[asp_vols[_]],
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use_channels=use_channels,
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flow_rates=[asp_flow_rates[_]] if asp_flow_rates and len(asp_flow_rates) > _ else None,
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offsets=[offsets[_]] if offsets and len(offsets) > _ else None,
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liquid_height=[liquid_height[_]] if liquid_height and len(liquid_height) > _ else None,
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blow_out_air_volume=[blow_out_air_volume[_]] if blow_out_air_volume and len(blow_out_air_volume) > _ else None,
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spread=spread,
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)
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if delays is not None:
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await self.custom_delay(seconds=delays[0])
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await self.dispense(
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resources=[targets[_]],
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vols=[dis_vols[_]],
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use_channels=use_channels,
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flow_rates=[dis_flow_rates[_]] if dis_flow_rates and len(dis_flow_rates) > _ else None,
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offsets=[offsets[_]] if offsets and len(offsets) > _ else None,
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blow_out_air_volume=[blow_out_air_volume[_]] if blow_out_air_volume and len(blow_out_air_volume) > _ else None,
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liquid_height=[liquid_height[_]] if liquid_height and len(liquid_height) > _ else None,
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spread=spread,
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)
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if delays is not None and len(delays) > 1:
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await self.custom_delay(seconds=delays[1])
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if mix_stage in ["after", "both"] and mix_times is not None and mix_times > 0:
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await self.mix(
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targets=[targets[_]],
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mix_time=mix_times,
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@@ -1004,63 +1097,60 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
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height_to_bottom=mix_liquid_height if mix_liquid_height else None,
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mix_rate=mix_rate if mix_rate else None,
|
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)
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if delays is not None:
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await self.custom_delay(seconds=delays[1])
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await self.touch_tip(targets[_])
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await self.discard_tips()
|
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if delays is not None and len(delays) > 1:
|
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await self.custom_delay(seconds=delays[1])
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await self.touch_tip(targets[_])
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await self.discard_tips(use_channels=use_channels)
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elif len(use_channels) == 8:
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# 对于8个的情况,需要判断此时任务是不是能被8通道移液站来成功处理
|
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if len(targets) % 8 != 0:
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raise ValueError(f"Length of `targets` {len(targets)} must be a multiple of 8 for 8-channel mode.")
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elif len(use_channels) == 8:
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if len(targets) % 8 != 0:
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raise ValueError(f"Length of `targets` {len(targets)} must be a multiple of 8 for 8-channel mode.")
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# 8个8个来取任务序列
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for i in range(0, len(targets), 8):
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tip = []
|
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for _ in range(len(use_channels)):
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tip.extend(next(self.current_tip))
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await self.pick_up_tips(tip)
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current_targets = targets[i:i + 8]
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current_reagent_sources = sources[i:i + 8]
|
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current_asp_vols = asp_vols[i:i + 8]
|
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current_dis_vols = dis_vols[i:i + 8]
|
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current_asp_flow_rates = asp_flow_rates[i:i + 8] if asp_flow_rates else None
|
||||
current_asp_offset = offsets[i:i + 8] if offsets else [None] * 8
|
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current_dis_offset = offsets[i:i + 8] if offsets else [None] * 8
|
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current_asp_liquid_height = liquid_height[i:i + 8] if liquid_height else [None] * 8
|
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current_dis_liquid_height = liquid_height[i:i + 8] if liquid_height else [None] * 8
|
||||
current_asp_blow_out_air_volume = blow_out_air_volume[i:i + 8] if blow_out_air_volume else [None] * 8
|
||||
current_dis_blow_out_air_volume = blow_out_air_volume[i:i + 8] if blow_out_air_volume else [None] * 8
|
||||
current_dis_flow_rates = dis_flow_rates[i:i + 8] if dis_flow_rates else None
|
||||
|
||||
for i in range(0, len(targets), 8):
|
||||
# 取出8个任务
|
||||
tip = []
|
||||
for _ in range(len(use_channels)):
|
||||
tip.extend(next(self.current_tip))
|
||||
await self.pick_up_tips(tip)
|
||||
current_targets = targets[i:i + 8]
|
||||
current_reagent_sources = sources[i:i + 8]
|
||||
current_asp_vols = asp_vols[i:i + 8]
|
||||
current_dis_vols = dis_vols[i:i + 8]
|
||||
current_asp_flow_rates = asp_flow_rates[i:i + 8]
|
||||
current_asp_offset = offsets[i:i + 8] if offsets else [None] * 8
|
||||
current_dis_offset = offsets[-i*8-8:len(offsets)-i*8] if offsets else [None] * 8
|
||||
current_asp_liquid_height = liquid_height[i:i + 8] if liquid_height else [None] * 8
|
||||
current_dis_liquid_height = liquid_height[-i*8-8:len(liquid_height)-i*8] if liquid_height else [None] * 8
|
||||
current_asp_blow_out_air_volume = blow_out_air_volume[i:i + 8] if blow_out_air_volume else [None] * 8
|
||||
current_dis_blow_out_air_volume = blow_out_air_volume[-i*8-8:len(blow_out_air_volume)-i*8] if blow_out_air_volume else [None] * 8
|
||||
current_dis_flow_rates = dis_flow_rates[i:i + 8] if dis_flow_rates else [None] * 8
|
||||
await self.aspirate(
|
||||
resources=current_reagent_sources,
|
||||
vols=current_asp_vols,
|
||||
use_channels=use_channels,
|
||||
flow_rates=current_asp_flow_rates,
|
||||
offsets=current_asp_offset,
|
||||
blow_out_air_volume=current_asp_blow_out_air_volume,
|
||||
liquid_height=current_asp_liquid_height,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
await self.aspirate(
|
||||
resources=current_reagent_sources,
|
||||
vols=current_asp_vols,
|
||||
use_channels=use_channels,
|
||||
flow_rates=current_asp_flow_rates,
|
||||
offsets=current_asp_offset,
|
||||
blow_out_air_volume=current_asp_blow_out_air_volume,
|
||||
liquid_height=current_asp_liquid_height,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
if delays is not None:
|
||||
await self.custom_delay(seconds=delays[0])
|
||||
await self.dispense(
|
||||
resources=current_targets,
|
||||
vols=current_dis_vols,
|
||||
use_channels=use_channels,
|
||||
flow_rates=current_dis_flow_rates,
|
||||
offsets=current_dis_offset,
|
||||
blow_out_air_volume=current_dis_blow_out_air_volume,
|
||||
liquid_height=current_dis_liquid_height,
|
||||
spread=spread,
|
||||
)
|
||||
if delays is not None:
|
||||
await self.custom_delay(seconds=delays[1])
|
||||
if delays is not None:
|
||||
await self.custom_delay(seconds=delays[0])
|
||||
await self.dispense(
|
||||
resources=current_targets,
|
||||
vols=current_dis_vols,
|
||||
use_channels=use_channels,
|
||||
flow_rates=current_dis_flow_rates,
|
||||
offsets=current_dis_offset,
|
||||
blow_out_air_volume=current_dis_blow_out_air_volume,
|
||||
liquid_height=current_dis_liquid_height,
|
||||
spread=spread,
|
||||
)
|
||||
if delays is not None and len(delays) > 1:
|
||||
await self.custom_delay(seconds=delays[1])
|
||||
|
||||
if mix_stage in ["after", "both"] and mix_times is not None and mix_times > 0:
|
||||
await self.mix(
|
||||
targets=current_targets,
|
||||
mix_time=mix_times,
|
||||
@@ -1069,10 +1159,363 @@ class LiquidHandlerAbstract(LiquidHandlerMiddleware):
|
||||
height_to_bottom=mix_liquid_height if mix_liquid_height else None,
|
||||
mix_rate=mix_rate if mix_rate else None,
|
||||
)
|
||||
if delays is not None:
|
||||
await self.custom_delay(seconds=delays[1])
|
||||
if delays is not None and len(delays) > 1:
|
||||
await self.custom_delay(seconds=delays[1])
|
||||
await self.touch_tip(current_targets)
|
||||
await self.discard_tips([0,1,2,3,4,5,6,7])
|
||||
|
||||
async def _transfer_one_to_many(
|
||||
self,
|
||||
source: Container,
|
||||
targets: Sequence[Container],
|
||||
tip_racks: Sequence[TipRack],
|
||||
use_channels: List[int],
|
||||
asp_vols: List[float],
|
||||
dis_vols: List[float],
|
||||
asp_flow_rates: Optional[List[Optional[float]]],
|
||||
dis_flow_rates: Optional[List[Optional[float]]],
|
||||
offsets: Optional[List[Coordinate]],
|
||||
touch_tip: bool,
|
||||
liquid_height: Optional[List[Optional[float]]],
|
||||
blow_out_air_volume: Optional[List[Optional[float]]],
|
||||
spread: Literal["wide", "tight", "custom"],
|
||||
mix_stage: Optional[Literal["none", "before", "after", "both"]],
|
||||
mix_times: Optional[int],
|
||||
mix_vol: Optional[int],
|
||||
mix_rate: Optional[int],
|
||||
mix_liquid_height: Optional[float],
|
||||
delays: Optional[List[int]],
|
||||
):
|
||||
"""一对多传输模式:1 source -> N targets"""
|
||||
# 验证和扩展体积参数
|
||||
if len(asp_vols) == 1:
|
||||
# 如果只提供一个吸液体积,计算总吸液体积(所有分液体积之和)
|
||||
total_asp_vol = sum(dis_vols)
|
||||
asp_vol = asp_vols[0] if asp_vols[0] >= total_asp_vol else total_asp_vol
|
||||
else:
|
||||
raise ValueError("For one-to-many mode, `asp_vols` should be a single value or list with one element.")
|
||||
|
||||
if len(dis_vols) != len(targets):
|
||||
raise ValueError(f"Length of `dis_vols` {len(dis_vols)} must match `targets` {len(targets)}.")
|
||||
|
||||
if len(use_channels) == 1:
|
||||
# 单通道模式:一次吸液,多次分液
|
||||
tip = []
|
||||
for _ in range(len(use_channels)):
|
||||
tip.extend(next(self.current_tip))
|
||||
await self.pick_up_tips(tip)
|
||||
|
||||
# 从源容器吸液(总体积)
|
||||
await self.aspirate(
|
||||
resources=[source],
|
||||
vols=[asp_vol],
|
||||
use_channels=use_channels,
|
||||
flow_rates=[asp_flow_rates[0]] if asp_flow_rates and len(asp_flow_rates) > 0 else None,
|
||||
offsets=[offsets[0]] if offsets and len(offsets) > 0 else None,
|
||||
liquid_height=[liquid_height[0]] if liquid_height and len(liquid_height) > 0 else None,
|
||||
blow_out_air_volume=[blow_out_air_volume[0]] if blow_out_air_volume and len(blow_out_air_volume) > 0 else None,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
if delays is not None:
|
||||
await self.custom_delay(seconds=delays[0])
|
||||
|
||||
# 分多次分液到不同的目标容器
|
||||
for idx, target in enumerate(targets):
|
||||
await self.dispense(
|
||||
resources=[target],
|
||||
vols=[dis_vols[idx]],
|
||||
use_channels=use_channels,
|
||||
flow_rates=[dis_flow_rates[idx]] if dis_flow_rates and len(dis_flow_rates) > idx else None,
|
||||
offsets=[offsets[idx]] if offsets and len(offsets) > idx else None,
|
||||
blow_out_air_volume=[blow_out_air_volume[idx]] if blow_out_air_volume and len(blow_out_air_volume) > idx else None,
|
||||
liquid_height=[liquid_height[idx]] if liquid_height and len(liquid_height) > idx else None,
|
||||
spread=spread,
|
||||
)
|
||||
if delays is not None and len(delays) > 1:
|
||||
await self.custom_delay(seconds=delays[1])
|
||||
if mix_stage in ["after", "both"] and mix_times is not None and mix_times > 0:
|
||||
await self.mix(
|
||||
targets=[target],
|
||||
mix_time=mix_times,
|
||||
mix_vol=mix_vol,
|
||||
offsets=offsets[idx:idx+1] if offsets else None,
|
||||
height_to_bottom=mix_liquid_height if mix_liquid_height else None,
|
||||
mix_rate=mix_rate if mix_rate else None,
|
||||
)
|
||||
if touch_tip:
|
||||
await self.touch_tip([target])
|
||||
|
||||
await self.discard_tips(use_channels=use_channels)
|
||||
|
||||
elif len(use_channels) == 8:
|
||||
# 8通道模式:需要确保目标数量是8的倍数
|
||||
if len(targets) % 8 != 0:
|
||||
raise ValueError(f"For 8-channel mode, number of targets {len(targets)} must be a multiple of 8.")
|
||||
|
||||
# 每次处理8个目标
|
||||
for i in range(0, len(targets), 8):
|
||||
tip = []
|
||||
for _ in range(len(use_channels)):
|
||||
tip.extend(next(self.current_tip))
|
||||
await self.pick_up_tips(tip)
|
||||
|
||||
current_targets = targets[i:i + 8]
|
||||
current_dis_vols = dis_vols[i:i + 8]
|
||||
|
||||
# 8个通道都从同一个源容器吸液,每个通道的吸液体积等于对应的分液体积
|
||||
current_asp_flow_rates = asp_flow_rates[0:1] * 8 if asp_flow_rates and len(asp_flow_rates) > 0 else None
|
||||
current_asp_offset = offsets[0:1] * 8 if offsets and len(offsets) > 0 else [None] * 8
|
||||
current_asp_liquid_height = liquid_height[0:1] * 8 if liquid_height and len(liquid_height) > 0 else [None] * 8
|
||||
current_asp_blow_out_air_volume = blow_out_air_volume[0:1] * 8 if blow_out_air_volume and len(blow_out_air_volume) > 0 else [None] * 8
|
||||
|
||||
# 从源容器吸液(8个通道都从同一个源,但每个通道的吸液体积不同)
|
||||
await self.aspirate(
|
||||
resources=[source] * 8, # 8个通道都从同一个源
|
||||
vols=current_dis_vols, # 每个通道的吸液体积等于对应的分液体积
|
||||
use_channels=use_channels,
|
||||
flow_rates=current_asp_flow_rates,
|
||||
offsets=current_asp_offset,
|
||||
liquid_height=current_asp_liquid_height,
|
||||
blow_out_air_volume=current_asp_blow_out_air_volume,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
if delays is not None:
|
||||
await self.custom_delay(seconds=delays[0])
|
||||
|
||||
# 分液到8个目标
|
||||
current_dis_flow_rates = dis_flow_rates[i:i + 8] if dis_flow_rates else None
|
||||
current_dis_offset = offsets[i:i + 8] if offsets else [None] * 8
|
||||
current_dis_liquid_height = liquid_height[i:i + 8] if liquid_height else [None] * 8
|
||||
current_dis_blow_out_air_volume = blow_out_air_volume[i:i + 8] if blow_out_air_volume else [None] * 8
|
||||
|
||||
await self.dispense(
|
||||
resources=current_targets,
|
||||
vols=current_dis_vols,
|
||||
use_channels=use_channels,
|
||||
flow_rates=current_dis_flow_rates,
|
||||
offsets=current_dis_offset,
|
||||
blow_out_air_volume=current_dis_blow_out_air_volume,
|
||||
liquid_height=current_dis_liquid_height,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
if delays is not None and len(delays) > 1:
|
||||
await self.custom_delay(seconds=delays[1])
|
||||
|
||||
if mix_stage in ["after", "both"] and mix_times is not None and mix_times > 0:
|
||||
await self.mix(
|
||||
targets=current_targets,
|
||||
mix_time=mix_times,
|
||||
mix_vol=mix_vol,
|
||||
offsets=offsets if offsets else None,
|
||||
height_to_bottom=mix_liquid_height if mix_liquid_height else None,
|
||||
mix_rate=mix_rate if mix_rate else None,
|
||||
)
|
||||
|
||||
if touch_tip:
|
||||
await self.touch_tip(current_targets)
|
||||
await self.discard_tips([0,1,2,3,4,5,6,7])
|
||||
|
||||
await self.discard_tips([0,1,2,3,4,5,6,7])
|
||||
|
||||
async def _transfer_many_to_one(
|
||||
self,
|
||||
sources: Sequence[Container],
|
||||
target: Container,
|
||||
tip_racks: Sequence[TipRack],
|
||||
use_channels: List[int],
|
||||
asp_vols: List[float],
|
||||
dis_vols: List[float],
|
||||
asp_flow_rates: Optional[List[Optional[float]]],
|
||||
dis_flow_rates: Optional[List[Optional[float]]],
|
||||
offsets: Optional[List[Coordinate]],
|
||||
touch_tip: bool,
|
||||
liquid_height: Optional[List[Optional[float]]],
|
||||
blow_out_air_volume: Optional[List[Optional[float]]],
|
||||
spread: Literal["wide", "tight", "custom"],
|
||||
mix_stage: Optional[Literal["none", "before", "after", "both"]],
|
||||
mix_times: Optional[int],
|
||||
mix_vol: Optional[int],
|
||||
mix_rate: Optional[int],
|
||||
mix_liquid_height: Optional[float],
|
||||
delays: Optional[List[int]],
|
||||
):
|
||||
"""多对一传输模式:N sources -> 1 target(汇总/混合)"""
|
||||
# 验证和扩展体积参数
|
||||
if len(asp_vols) != len(sources):
|
||||
raise ValueError(f"Length of `asp_vols` {len(asp_vols)} must match `sources` {len(sources)}.")
|
||||
|
||||
# 支持两种模式:
|
||||
# 1. dis_vols 为单个值:所有源汇总,使用总吸液体积或指定分液体积
|
||||
# 2. dis_vols 长度等于 asp_vols:每个源按不同比例分液(按比例混合)
|
||||
if len(dis_vols) == 1:
|
||||
# 模式1:使用单个分液体积
|
||||
total_dis_vol = sum(asp_vols)
|
||||
dis_vol = dis_vols[0] if dis_vols[0] >= total_dis_vol else total_dis_vol
|
||||
use_proportional_mixing = False
|
||||
elif len(dis_vols) == len(asp_vols):
|
||||
# 模式2:按不同比例混合
|
||||
use_proportional_mixing = True
|
||||
else:
|
||||
raise ValueError(
|
||||
f"For many-to-one mode, `dis_vols` should be a single value or list with length {len(asp_vols)} "
|
||||
f"(matching `asp_vols`). Got length {len(dis_vols)}."
|
||||
)
|
||||
|
||||
if len(use_channels) == 1:
|
||||
# 单通道模式:多次吸液,一次分液
|
||||
# 先混合前(如果需要)
|
||||
if mix_stage in ["before", "both"] and mix_times is not None and mix_times > 0:
|
||||
# 注意:在吸液前混合源容器通常不常见,这里跳过
|
||||
pass
|
||||
|
||||
# 从每个源容器吸液并分液到目标容器
|
||||
for idx, source in enumerate(sources):
|
||||
tip = []
|
||||
for _ in range(len(use_channels)):
|
||||
tip.extend(next(self.current_tip))
|
||||
await self.pick_up_tips(tip)
|
||||
|
||||
await self.aspirate(
|
||||
resources=[source],
|
||||
vols=[asp_vols[idx]],
|
||||
use_channels=use_channels,
|
||||
flow_rates=[asp_flow_rates[idx]] if asp_flow_rates and len(asp_flow_rates) > idx else None,
|
||||
offsets=[offsets[idx]] if offsets and len(offsets) > idx else None,
|
||||
liquid_height=[liquid_height[idx]] if liquid_height and len(liquid_height) > idx else None,
|
||||
blow_out_air_volume=[blow_out_air_volume[idx]] if blow_out_air_volume and len(blow_out_air_volume) > idx else None,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
if delays is not None:
|
||||
await self.custom_delay(seconds=delays[0])
|
||||
|
||||
# 分液到目标容器
|
||||
if use_proportional_mixing:
|
||||
# 按不同比例混合:使用对应的 dis_vols
|
||||
dis_vol = dis_vols[idx]
|
||||
dis_flow_rate = dis_flow_rates[idx] if dis_flow_rates and len(dis_flow_rates) > idx else None
|
||||
dis_offset = offsets[idx] if offsets and len(offsets) > idx else None
|
||||
dis_liquid_height = liquid_height[idx] if liquid_height and len(liquid_height) > idx else None
|
||||
dis_blow_out = blow_out_air_volume[idx] if blow_out_air_volume and len(blow_out_air_volume) > idx else None
|
||||
else:
|
||||
# 标准模式:分液体积等于吸液体积
|
||||
dis_vol = asp_vols[idx]
|
||||
dis_flow_rate = dis_flow_rates[0] if dis_flow_rates and len(dis_flow_rates) > 0 else None
|
||||
dis_offset = offsets[0] if offsets and len(offsets) > 0 else None
|
||||
dis_liquid_height = liquid_height[0] if liquid_height and len(liquid_height) > 0 else None
|
||||
dis_blow_out = blow_out_air_volume[0] if blow_out_air_volume and len(blow_out_air_volume) > 0 else None
|
||||
|
||||
await self.dispense(
|
||||
resources=[target],
|
||||
vols=[dis_vol],
|
||||
use_channels=use_channels,
|
||||
flow_rates=[dis_flow_rate] if dis_flow_rate is not None else None,
|
||||
offsets=[dis_offset] if dis_offset is not None else None,
|
||||
blow_out_air_volume=[dis_blow_out] if dis_blow_out is not None else None,
|
||||
liquid_height=[dis_liquid_height] if dis_liquid_height is not None else None,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
if delays is not None and len(delays) > 1:
|
||||
await self.custom_delay(seconds=delays[1])
|
||||
|
||||
await self.discard_tips(use_channels=use_channels)
|
||||
|
||||
# 最后在目标容器中混合(如果需要)
|
||||
if mix_stage in ["after", "both"] and mix_times is not None and mix_times > 0:
|
||||
await self.mix(
|
||||
targets=[target],
|
||||
mix_time=mix_times,
|
||||
mix_vol=mix_vol,
|
||||
offsets=offsets[0:1] if offsets else None,
|
||||
height_to_bottom=mix_liquid_height if mix_liquid_height else None,
|
||||
mix_rate=mix_rate if mix_rate else None,
|
||||
)
|
||||
|
||||
if touch_tip:
|
||||
await self.touch_tip([target])
|
||||
|
||||
elif len(use_channels) == 8:
|
||||
# 8通道模式:需要确保源数量是8的倍数
|
||||
if len(sources) % 8 != 0:
|
||||
raise ValueError(f"For 8-channel mode, number of sources {len(sources)} must be a multiple of 8.")
|
||||
|
||||
# 每次处理8个源
|
||||
for i in range(0, len(sources), 8):
|
||||
tip = []
|
||||
for _ in range(len(use_channels)):
|
||||
tip.extend(next(self.current_tip))
|
||||
await self.pick_up_tips(tip)
|
||||
|
||||
current_sources = sources[i:i + 8]
|
||||
current_asp_vols = asp_vols[i:i + 8]
|
||||
current_asp_flow_rates = asp_flow_rates[i:i + 8] if asp_flow_rates else None
|
||||
current_asp_offset = offsets[i:i + 8] if offsets else [None] * 8
|
||||
current_asp_liquid_height = liquid_height[i:i + 8] if liquid_height else [None] * 8
|
||||
current_asp_blow_out_air_volume = blow_out_air_volume[i:i + 8] if blow_out_air_volume else [None] * 8
|
||||
|
||||
# 从8个源容器吸液
|
||||
await self.aspirate(
|
||||
resources=current_sources,
|
||||
vols=current_asp_vols,
|
||||
use_channels=use_channels,
|
||||
flow_rates=current_asp_flow_rates,
|
||||
offsets=current_asp_offset,
|
||||
blow_out_air_volume=current_asp_blow_out_air_volume,
|
||||
liquid_height=current_asp_liquid_height,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
if delays is not None:
|
||||
await self.custom_delay(seconds=delays[0])
|
||||
|
||||
# 分液到目标容器(每个通道分液到同一个目标)
|
||||
if use_proportional_mixing:
|
||||
# 按比例混合:使用对应的 dis_vols
|
||||
current_dis_vols = dis_vols[i:i + 8]
|
||||
current_dis_flow_rates = dis_flow_rates[i:i + 8] if dis_flow_rates else None
|
||||
current_dis_offset = offsets[i:i + 8] if offsets else [None] * 8
|
||||
current_dis_liquid_height = liquid_height[i:i + 8] if liquid_height else [None] * 8
|
||||
current_dis_blow_out_air_volume = blow_out_air_volume[i:i + 8] if blow_out_air_volume else [None] * 8
|
||||
else:
|
||||
# 标准模式:每个通道分液体积等于其吸液体积
|
||||
current_dis_vols = current_asp_vols
|
||||
current_dis_flow_rates = dis_flow_rates[0:1] * 8 if dis_flow_rates else None
|
||||
current_dis_offset = offsets[0:1] * 8 if offsets else [None] * 8
|
||||
current_dis_liquid_height = liquid_height[0:1] * 8 if liquid_height else [None] * 8
|
||||
current_dis_blow_out_air_volume = blow_out_air_volume[0:1] * 8 if blow_out_air_volume else [None] * 8
|
||||
|
||||
await self.dispense(
|
||||
resources=[target] * 8, # 8个通道都分到同一个目标
|
||||
vols=current_dis_vols,
|
||||
use_channels=use_channels,
|
||||
flow_rates=current_dis_flow_rates,
|
||||
offsets=current_dis_offset,
|
||||
blow_out_air_volume=current_dis_blow_out_air_volume,
|
||||
liquid_height=current_dis_liquid_height,
|
||||
spread=spread,
|
||||
)
|
||||
|
||||
if delays is not None and len(delays) > 1:
|
||||
await self.custom_delay(seconds=delays[1])
|
||||
|
||||
await self.discard_tips([0,1,2,3,4,5,6,7])
|
||||
|
||||
# 最后在目标容器中混合(如果需要)
|
||||
if mix_stage in ["after", "both"] and mix_times is not None and mix_times > 0:
|
||||
await self.mix(
|
||||
targets=[target],
|
||||
mix_time=mix_times,
|
||||
mix_vol=mix_vol,
|
||||
offsets=offsets[0:1] if offsets else None,
|
||||
height_to_bottom=mix_liquid_height if mix_liquid_height else None,
|
||||
mix_rate=mix_rate if mix_rate else None,
|
||||
)
|
||||
|
||||
if touch_tip:
|
||||
await self.touch_tip([target])
|
||||
|
||||
# except Exception as e:
|
||||
# traceback.print_exc()
|
||||
|
||||
@@ -7,7 +7,7 @@ class VirtualMultiwayValve:
|
||||
"""
|
||||
虚拟九通阀门 - 0号位连接transfer pump,1-8号位连接其他设备 🔄
|
||||
"""
|
||||
def __init__(self, port: str = "VIRTUAL", positions: int = 8):
|
||||
def __init__(self, port: str = "VIRTUAL", positions: int = 8, **kwargs):
|
||||
self.port = port
|
||||
self.max_positions = positions # 1-8号位
|
||||
self.total_positions = positions + 1 # 0-8号位,共9个位置
|
||||
|
||||
@@ -147,7 +147,7 @@ class WorkstationBase(ABC):
|
||||
|
||||
def __init__(
|
||||
self,
|
||||
deck: Deck,
|
||||
deck: Optional[Deck],
|
||||
*args,
|
||||
**kwargs, # 必须有kwargs
|
||||
):
|
||||
@@ -349,5 +349,5 @@ class WorkstationBase(ABC):
|
||||
|
||||
|
||||
class ProtocolNode(WorkstationBase):
|
||||
def __init__(self, deck: Optional[PLRResource], *args, **kwargs):
|
||||
def __init__(self, protocol_type: List[str], deck: Optional[PLRResource], *args, **kwargs):
|
||||
super().__init__(deck, *args, **kwargs)
|
||||
|
||||
@@ -6036,7 +6036,12 @@ workstation:
|
||||
properties:
|
||||
deck:
|
||||
type: string
|
||||
protocol_type:
|
||||
items:
|
||||
type: string
|
||||
type: array
|
||||
required:
|
||||
- protocol_type
|
||||
- deck
|
||||
type: object
|
||||
data:
|
||||
|
||||
@@ -2,7 +2,7 @@ container:
|
||||
category:
|
||||
- container
|
||||
class:
|
||||
module: unilabos.resources.container:RegularContainer
|
||||
module: unilabos.resources.container:get_regular_container
|
||||
type: pylabrobot
|
||||
description: regular organic container
|
||||
handles:
|
||||
|
||||
@@ -22,6 +22,13 @@ class RegularContainer(Container):
|
||||
|
||||
def load_state(self, state: Dict[str, Any]):
|
||||
self.state = state
|
||||
|
||||
|
||||
def get_regular_container(name="container"):
|
||||
r = RegularContainer(name=name)
|
||||
r.category = "container"
|
||||
return RegularContainer(name=name)
|
||||
|
||||
#
|
||||
# class RegularContainer(object):
|
||||
# # 第一个参数必须是id传入
|
||||
|
||||
@@ -1144,7 +1144,7 @@ class BaseROS2DeviceNode(Node, Generic[T]):
|
||||
queried_resources = []
|
||||
for resource_data in resource_inputs:
|
||||
plr_resource = await self.get_resource_with_dir(
|
||||
resource_ids=resource_data["id"], with_children=True
|
||||
resource_id=resource_data["id"], with_children=True
|
||||
)
|
||||
queried_resources.append(plr_resource)
|
||||
|
||||
|
||||
@@ -124,11 +124,14 @@ class ColoredFormatter(logging.Formatter):
|
||||
def _format_basic(self, record):
|
||||
"""基本格式化,不包含颜色"""
|
||||
datetime_str = datetime.fromtimestamp(record.created).strftime("%y-%m-%d [%H:%M:%S,%f")[:-3] + "]"
|
||||
filename = os.path.basename(record.filename).rsplit(".", 1)[0] # 提取文件名(不含路径和扩展名)
|
||||
filename = record.filename.replace(".py", "").split("\\")[-1] # 提取文件名(不含路径和扩展名)
|
||||
if "/" in filename:
|
||||
filename = filename.split("/")[-1]
|
||||
module_path = f"{record.name}.{filename}"
|
||||
func_line = f"{record.funcName}:{record.lineno}"
|
||||
right_info = f" [{func_line}] [{module_path}]"
|
||||
|
||||
formatted_message = f"{datetime_str} [{record.levelname}] [{module_path}] [{func_line}]: {record.getMessage()}"
|
||||
formatted_message = f"{datetime_str} [{record.levelname}] {record.getMessage()}{right_info}"
|
||||
|
||||
if record.exc_info:
|
||||
exc_text = self.formatException(record.exc_info)
|
||||
@@ -150,7 +153,7 @@ class ColoredFormatter(logging.Formatter):
|
||||
|
||||
|
||||
# 配置日志处理器
|
||||
def configure_logger(loglevel=None):
|
||||
def configure_logger(loglevel=None, working_dir=None):
|
||||
"""配置日志记录器
|
||||
|
||||
Args:
|
||||
@@ -191,9 +194,30 @@ def configure_logger(loglevel=None):
|
||||
|
||||
# 添加处理器到根日志记录器
|
||||
root_logger.addHandler(console_handler)
|
||||
|
||||
# 如果指定了工作目录,添加文件处理器
|
||||
if working_dir is not None:
|
||||
logs_dir = os.path.join(working_dir, "logs")
|
||||
os.makedirs(logs_dir, exist_ok=True)
|
||||
|
||||
# 生成日志文件名:日期 时间.log
|
||||
log_filename = datetime.now().strftime("%Y-%m-%d %H-%M-%S") + ".log"
|
||||
log_filepath = os.path.join(logs_dir, log_filename)
|
||||
|
||||
# 创建文件处理器
|
||||
file_handler = logging.FileHandler(log_filepath, encoding="utf-8")
|
||||
file_handler.setLevel(root_logger.level)
|
||||
|
||||
# 使用不带颜色的格式化器
|
||||
file_formatter = ColoredFormatter(use_colors=False)
|
||||
file_handler.setFormatter(file_formatter)
|
||||
|
||||
root_logger.addHandler(file_handler)
|
||||
|
||||
logging.getLogger("asyncio").setLevel(logging.INFO)
|
||||
logging.getLogger("urllib3").setLevel(logging.INFO)
|
||||
|
||||
|
||||
# 配置日志系统
|
||||
configure_logger()
|
||||
|
||||
|
||||
Reference in New Issue
Block a user