mirror of
https://github.com/dptech-corp/Uni-Lab-OS.git
synced 2025-12-17 13:01:12 +00:00
622 lines
25 KiB
Python
622 lines
25 KiB
Python
import asyncio
|
||
import collections
|
||
import contextlib
|
||
import json
|
||
import socket
|
||
import time
|
||
from typing import Any, List, Dict, Optional, TypedDict, Union, Sequence, Iterator, Literal
|
||
import pprint as pp
|
||
from pylabrobot.liquid_handling import (
|
||
LiquidHandlerBackend,
|
||
Pickup,
|
||
SingleChannelAspiration,
|
||
Drop,
|
||
SingleChannelDispense,
|
||
PickupTipRack,
|
||
DropTipRack,
|
||
MultiHeadAspirationPlate, ChatterBoxBackend, LiquidHandlerChatterboxBackend,
|
||
)
|
||
from pylabrobot.liquid_handling.standard import (
|
||
MultiHeadAspirationContainer,
|
||
MultiHeadDispenseContainer,
|
||
MultiHeadDispensePlate,
|
||
ResourcePickup,
|
||
ResourceMove,
|
||
ResourceDrop,
|
||
)
|
||
from pylabrobot.resources import Tip, Deck, Plate, Well, TipRack, Resource, Container, Coordinate, TipSpot, Trash
|
||
from traitlets import Int
|
||
|
||
from unilabos.devices.liquid_handling.liquid_handler_abstract import LiquidHandlerAbstract
|
||
|
||
|
||
|
||
|
||
class MaterialResource:
|
||
"""统一的液体/反应器资源，支持多孔（wells）场景：
|
||
- wells: 列表，每个元素代表一个物料孔（unit）；
|
||
- units: 与 wells 对齐的列表，每个元素是 {liquid_id: volume}；
|
||
- 若传入 liquid_id + volume 或 composition，总量将**等分**到各 unit；
|
||
"""
|
||
def __init__(
|
||
self,
|
||
resource_name: str,
|
||
slot: int,
|
||
well: List[int],
|
||
composition: Optional[Dict[str, float]] = None,
|
||
liquid_id: Optional[str] = None,
|
||
volume: Union[float, int] = 0.0,
|
||
is_supply: Optional[bool] = None,
|
||
):
|
||
self.resource_name = resource_name
|
||
self.slot = int(slot)
|
||
self.well = list(well or [])
|
||
self.is_supply = bool(is_supply) if is_supply is not None else (bool(composition) or (liquid_id is not None))
|
||
|
||
# 规范化：至少有 1 个 unit
|
||
n = max(1, len(self.well))
|
||
self.units: List[Dict[str, float]] = [dict() for _ in range(n)]
|
||
|
||
# 初始化内容：等分到各 unit
|
||
if composition:
|
||
for k, v in composition.items():
|
||
share = float(v) / n
|
||
for u in self.units:
|
||
if share > 0:
|
||
u[k] = u.get(k, 0.0) + share
|
||
elif liquid_id is not None and float(volume) > 0:
|
||
share = float(volume) / n
|
||
for u in self.units:
|
||
u[liquid_id] = u.get(liquid_id, 0.0) + share
|
||
|
||
# 位置描述
|
||
def location(self) -> Dict[str, Any]:
|
||
return {"slot": self.slot, "well": self.well}
|
||
|
||
def unit_count(self) -> int:
|
||
return len(self.units)
|
||
|
||
def unit_volume(self, idx: int) -> float:
|
||
return float(sum(self.units[idx].values()))
|
||
|
||
def total_volume(self) -> float:
|
||
return float(sum(self.unit_volume(i) for i in range(self.unit_count())))
|
||
|
||
def add_to_unit(self, idx: int, liquid_id: str, vol: Union[float, int]):
|
||
v = float(vol)
|
||
if v < 0:
|
||
return
|
||
u = self.units[idx]
|
||
if liquid_id not in u:
|
||
u[liquid_id] = 0.0
|
||
if v > 0:
|
||
u[liquid_id] += v
|
||
|
||
def remove_from_unit(self, idx: int, total: Union[float, int]) -> Dict[str, float]:
|
||
take = float(total)
|
||
if take <= 0: return {}
|
||
u = self.units[idx]
|
||
avail = sum(u.values())
|
||
if avail <= 0: return {}
|
||
take = min(take, avail)
|
||
ratio = take / avail
|
||
removed: Dict[str, float] = {}
|
||
for k, v in list(u.items()):
|
||
dv = v * ratio
|
||
nv = v - dv
|
||
if nv < 1e-9: nv = 0.0
|
||
u[k] = nv
|
||
removed[k] = dv
|
||
|
||
self.units[idx] = {k: v for k, v in u.items() if v > 0}
|
||
return removed
|
||
|
||
def transfer_unit_to(self, src_idx: int, other: "MaterialResource", dst_idx: int, total: Union[float, int]):
|
||
moved = self.remove_from_unit(src_idx, total)
|
||
for k, v in moved.items():
|
||
other.add_to_unit(dst_idx, k, v)
|
||
|
||
def get_resource(self) -> Dict[str, Any]:
|
||
return {
|
||
"resource_name": self.resource_name,
|
||
"slot": self.slot,
|
||
"well": self.well,
|
||
"units": [dict(u) for u in self.units],
|
||
"total_volume": self.total_volume(),
|
||
"is_supply": self.is_supply,
|
||
}
|
||
|
||
def transfer_liquid(
|
||
sources: MaterialResource,
|
||
targets: MaterialResource,
|
||
unit_volume: Optional[Union[float, int]] = None,
|
||
tip: Optional[str] = None, #这里应该是指定种类的
|
||
) -> Dict[str, Any]:
|
||
try:
|
||
vol_each = float(unit_volume)
|
||
except (TypeError, ValueError):
|
||
return {"action": "transfer_liquid", "error": "invalid unit_volume"}
|
||
if vol_each <= 0:
|
||
return {"action": "transfer_liquid", "error": "non-positive volume"}
|
||
|
||
ns, nt = sources.unit_count(), targets.unit_count()
|
||
# one-to-many: 从单个 source unit(0) 扇出到目标各 unit
|
||
if ns == 1 and nt >= 1:
|
||
for j in range(nt):
|
||
sources.transfer_unit_to(0, targets, j, vol_each)
|
||
# many-to-many: 数量相同，逐一对应
|
||
elif ns == nt and ns > 0:
|
||
for i in range(ns):
|
||
sources.transfer_unit_to(i, targets, i, vol_each)
|
||
else:
|
||
raise ValueError(f"Unsupported mapping: sources={ns} units, targets={nt} units. Only 1->N or N->N are allowed.")
|
||
|
||
return {
|
||
"action": "transfer_liquid",
|
||
"sources": sources.get_resource(),
|
||
"targets": targets.get_resource(),
|
||
"unit_volume": unit_volume,
|
||
"tip": tip,
|
||
}
|
||
|
||
def plan_transfer(pm: "ProtocolManager", **kwargs) -> Dict[str, Any]:
|
||
"""Shorthand to add a non-committing transfer to a ProtocolManager.
|
||
Accepts the same kwargs as ProtocolManager.add_transfer.
|
||
"""
|
||
return pm.add_transfer(**kwargs)
|
||
|
||
class ProtocolManager:
|
||
"""Plan/track transfers and back‑solve minimum initial volumes.
|
||
|
||
Use add_transfer(...) to register steps (no mutation).
|
||
Use compute_min_initials(...) to infer the minimal starting volume of each liquid
|
||
per resource required to execute the plan in order.
|
||
"""
|
||
|
||
# ---------- lifecycle ----------
|
||
def __init__(self):
|
||
# queued logical steps (keep live refs to MaterialResource)
|
||
self.steps: List[Dict[str, Any]] = []
|
||
|
||
# simple tip catalog; choose the smallest that meets min_aspirate and capacity*safety
|
||
self.tip_catalog = [
|
||
{"name": "TIP_10uL", "capacity": 10.0, "min_aspirate": 0.5},
|
||
{"name": "TIP_20uL", "capacity": 20.0, "min_aspirate": 1.0},
|
||
{"name": "TIP_50uL", "capacity": 50.0, "min_aspirate": 2.0},
|
||
{"name": "TIP_200uL", "capacity": 200.0, "min_aspirate": 5.0},
|
||
{"name": "TIP_300uL", "capacity": 300.0, "min_aspirate": 10.0},
|
||
{"name": "TIP_1000uL", "capacity": 1000.0, "min_aspirate": 20.0},
|
||
]
|
||
|
||
# stable labels for unknown liquids per resource (A, B, C, ..., AA, AB, ...)
|
||
self._unknown_labels: Dict[MaterialResource, str] = {}
|
||
self._unknown_label_counter: int = 0
|
||
|
||
# ---------- public API ----------
|
||
def recommend_tip(self, unit_volume: float, safety: float = 1.10) -> str:
|
||
v = float(unit_volume)
|
||
# prefer: meets min_aspirate and capacity with safety margin; else fallback to capacity-only; else max capacity
|
||
eligible = [t for t in self.tip_catalog if t["min_aspirate"] <= v and t["capacity"] >= v * safety]
|
||
if not eligible:
|
||
eligible = [t for t in self.tip_catalog if t["capacity"] >= v]
|
||
return min(eligible or self.tip_catalog, key=lambda t: t["capacity"]) ["name"]
|
||
|
||
def get_tip_capacity(self, tip_name: str) -> Optional[float]:
|
||
for t in self.tip_catalog:
|
||
if t["name"] == tip_name:
|
||
return t["capacity"]
|
||
return None
|
||
|
||
def add_transfer(
|
||
self,
|
||
sources: MaterialResource,
|
||
targets: MaterialResource,
|
||
unit_volume: Union[float, int],
|
||
tip: Optional[str] = None,
|
||
) -> Dict[str, Any]:
|
||
step = {
|
||
"action": "transfer_liquid",
|
||
"sources": sources,
|
||
"targets": targets,
|
||
"unit_volume": float(unit_volume),
|
||
"tip": tip or self.recommend_tip(unit_volume),
|
||
}
|
||
self.steps.append(step)
|
||
# return a serializable shadow (no mutation)
|
||
return {
|
||
"action": "transfer_liquid",
|
||
"sources": sources.get_resource(),
|
||
"targets": targets.get_resource(),
|
||
"unit_volume": step["unit_volume"],
|
||
"tip": step["tip"],
|
||
}
|
||
|
||
@staticmethod
|
||
def _liquid_keys_of(resource: MaterialResource) -> List[str]:
|
||
keys: set[str] = set()
|
||
for u in resource.units:
|
||
keys.update(u.keys())
|
||
return sorted(keys)
|
||
|
||
@staticmethod
|
||
def _fanout_multiplier(ns: int, nt: int) -> Optional[int]:
|
||
"""Return the number of liquid movements for a mapping shape.
|
||
1->N: N moves; N->N: N moves; otherwise unsupported (None).
|
||
"""
|
||
if ns == 1 and nt >= 1:
|
||
return nt
|
||
if ns == nt and ns > 0:
|
||
return ns
|
||
return None
|
||
|
||
# ---------- planning core ----------
|
||
def compute_min_initials(
|
||
self,
|
||
use_initial: bool = False,
|
||
external_only: bool = True,
|
||
) -> Dict[str, Dict[str, float]]:
|
||
"""Simulate the plan (non‑mutating) and return minimal starting volumes per resource/liquid."""
|
||
ledger: Dict[MaterialResource, Dict[str, float]] = {}
|
||
min_seen: Dict[MaterialResource, Dict[str, float]] = {}
|
||
|
||
def _ensure(res: MaterialResource) -> None:
|
||
if res in ledger:
|
||
return
|
||
declared = self._liquid_keys_of(res)
|
||
if use_initial:
|
||
# sum actual held amounts across units
|
||
totals = {k: 0.0 for k in declared}
|
||
for u in res.units:
|
||
for k, v in u.items():
|
||
totals[k] = totals.get(k, 0.0) + float(v)
|
||
ledger[res] = totals
|
||
else:
|
||
ledger[res] = {k: 0.0 for k in declared}
|
||
min_seen[res] = {k: ledger[res].get(k, 0.0) for k in ledger[res]}
|
||
|
||
def _proportions(src: MaterialResource, src_bal: Dict[str, float]) -> tuple[List[str], Dict[str, float]]:
|
||
keys = list(src_bal.keys())
|
||
total_pos = sum(x for x in src_bal.values() if x > 0)
|
||
|
||
# if ledger has no keys yet, seed from declared types on the resource
|
||
if not keys:
|
||
keys = self._liquid_keys_of(src)
|
||
for k in keys:
|
||
src_bal.setdefault(k, 0.0)
|
||
min_seen[src].setdefault(k, 0.0)
|
||
|
||
if total_pos > 0:
|
||
# proportional to current positive balances
|
||
props = {k: (src_bal.get(k, 0.0) / total_pos) for k in keys}
|
||
return keys, props
|
||
|
||
# no material currently: evenly from known keys, or assign an unknown label
|
||
if keys:
|
||
eq = 1.0 / len(keys)
|
||
return keys, {k: eq for k in keys}
|
||
|
||
unk = self._label_for_unknown(src)
|
||
keys = [unk]
|
||
src_bal.setdefault(unk, 0.0)
|
||
min_seen[src].setdefault(unk, 0.0)
|
||
return keys, {unk: 1.0}
|
||
|
||
for step in self.steps:
|
||
if step.get("action") != "transfer_liquid":
|
||
continue
|
||
|
||
src: MaterialResource = step["sources"]
|
||
dst: MaterialResource = step["targets"]
|
||
vol = float(step["unit_volume"])
|
||
if vol <= 0:
|
||
continue
|
||
|
||
_ensure(src)
|
||
_ensure(dst)
|
||
|
||
mult = self._fanout_multiplier(src.unit_count(), dst.unit_count())
|
||
if not mult:
|
||
continue # unsupported mapping shape for this planner
|
||
|
||
eff_vol = vol * mult
|
||
src_bal = ledger[src]
|
||
keys, props = _proportions(src, src_bal)
|
||
|
||
# subtract from src; track minima; accumulate to dst
|
||
moved: Dict[str, float] = {}
|
||
for k in keys:
|
||
dv = eff_vol * props[k]
|
||
src_bal[k] = src_bal.get(k, 0.0) - dv
|
||
moved[k] = dv
|
||
prev_min = min_seen[src].get(k, 0.0)
|
||
if src_bal[k] < prev_min:
|
||
min_seen[src][k] = src_bal[k]
|
||
|
||
dst_bal = ledger[dst]
|
||
for k, dv in moved.items():
|
||
dst_bal[k] = dst_bal.get(k, 0.0) + dv
|
||
min_seen[dst].setdefault(k, dst_bal[k])
|
||
|
||
# convert minima (negative) to required initials
|
||
result: Dict[str, Dict[str, float]] = {}
|
||
for res, mins in min_seen.items():
|
||
if external_only and not getattr(res, "is_supply", False):
|
||
continue
|
||
need = {liq: max(0.0, -mn) for liq, mn in mins.items() if mn < 0.0}
|
||
if need:
|
||
result[res.resource_name] = need
|
||
return result
|
||
|
||
def compute_tip_consumption(self) -> Dict[str, Any]:
|
||
"""Compute how many tips are consumed at each transfer step, and aggregate by tip type.
|
||
Rule: each liquid movement (source unit -> target unit) consumes one tip.
|
||
For supported shapes: 1->N uses N tips; N->N uses N tips.
|
||
"""
|
||
per_step: List[Dict[str, Any]] = []
|
||
totals_by_tip: Dict[str, int] = {}
|
||
|
||
for i, s in enumerate(self.steps):
|
||
if s.get("action") != "transfer_liquid":
|
||
continue
|
||
ns = s["sources"].unit_count()
|
||
nt = s["targets"].unit_count()
|
||
moves = self._fanout_multiplier(ns, nt) or 0
|
||
tip_name = s.get("tip") or self.recommend_tip(s["unit_volume"]) # per-step tip may vary
|
||
per_step.append({
|
||
"idx": i,
|
||
"tip": tip_name,
|
||
"tips_used": moves,
|
||
"moves": moves,
|
||
})
|
||
totals_by_tip[tip_name] = totals_by_tip.get(tip_name, 0) + int(moves)
|
||
|
||
return {"per_step": per_step, "totals_by_tip": totals_by_tip}
|
||
|
||
def compute_min_initials_with_tips(
|
||
self,
|
||
use_initial: bool = False,
|
||
external_only: bool = True,
|
||
) -> Dict[str, Any]:
|
||
needs = self.compute_min_initials(use_initial=use_initial, external_only=external_only)
|
||
step_tips: List[Dict[str, Any]] = []
|
||
totals_by_tip: Dict[str, int] = {}
|
||
|
||
for i, s in enumerate(self.steps):
|
||
if s.get("action") != "transfer_liquid":
|
||
continue
|
||
ns = s["sources"].unit_count()
|
||
nt = s["targets"].unit_count()
|
||
moves = self._fanout_multiplier(ns, nt) or 0
|
||
tip_name = s.get("tip") or self.recommend_tip(s["unit_volume"]) # step-specific tip
|
||
totals_by_tip[self.get_tip_capacity(tip_name)] = totals_by_tip.get(tip_name, 0) + int(moves)
|
||
|
||
step_tips.append({
|
||
"idx": i,
|
||
"tip": tip_name,
|
||
"tip_capacity": self.get_tip_capacity(tip_name),
|
||
"unit_volume": s["unit_volume"],
|
||
"tips_used": moves,
|
||
})
|
||
return {"liquid_setup": needs, "step_tips": step_tips, "totals_by_tip": totals_by_tip}
|
||
|
||
# ---------- unknown labels ----------
|
||
def _index_to_letters(self, idx: int) -> str:
|
||
"""0->A, 1->B, ... 25->Z, 26->AA, 27->AB ... (Excel-like)"""
|
||
s: List[str] = []
|
||
idx = int(idx)
|
||
while True:
|
||
idx, r = divmod(idx, 26)
|
||
s.append(chr(ord('A') + r))
|
||
if idx == 0:
|
||
break
|
||
idx -= 1 # Excel-style carry
|
||
return "".join(reversed(s))
|
||
|
||
def _label_for_unknown(self, res: MaterialResource) -> str:
|
||
"""Assign a stable unknown-liquid label (A/B/C/...) per resource."""
|
||
if res not in self._unknown_labels:
|
||
lab = self._index_to_letters(self._unknown_label_counter)
|
||
self._unknown_label_counter += 1
|
||
self._unknown_labels[res] = lab
|
||
return self._unknown_labels[res]
|
||
|
||
|
||
# 在这一步传输目前有的物料
|
||
class LabResource:
|
||
def __init__(self):
|
||
self.tipracks = []
|
||
self.plates = []
|
||
self.trash = []
|
||
|
||
def add_tipracks(self, tiprack: List[TipRack]):
|
||
self.tipracks.extend(tiprack)
|
||
def add_plates(self, plate: List[Plate]):
|
||
self.plates.extend(plate)
|
||
def add_trash(self, trash: List[Plate]):
|
||
self.trash.extend(trash)
|
||
def get_resources_info(self) -> Dict[str, Any]:
|
||
tipracks = [{"name": tr.name, "max_volume": tr.children[0].tracker._tip.maximal_volume, "count": len(tr.children)} for tr in self.tipracks]
|
||
plates = [{"name": pl.name, "max_volume": pl.children[0].max_volume, "count": len(pl.children)} for pl in self.plates]
|
||
trash = [{"name": t.name, "max_volume": t.children[0].max_volume, "count": len(t.children)} for t in self.trash]
|
||
return {
|
||
"tipracks": tipracks,
|
||
"plates": plates,
|
||
"trash": trash
|
||
}
|
||
|
||
from typing import Dict, Any
|
||
|
||
import time
|
||
class DefaultLayout:
|
||
|
||
def __init__(self, product_name: str = "PRCXI9300"):
|
||
self.labresource = {}
|
||
if product_name not in ["PRCXI9300", "PRCXI9320"]:
|
||
raise ValueError(f"Unsupported product_name: {product_name}. Only 'PRCXI9300' and 'PRCXI9320' are supported.")
|
||
|
||
if product_name == "PRCXI9300":
|
||
self.rows = 2
|
||
self.columns = 3
|
||
self.layout = [1, 2, 3, 4, 5, 6]
|
||
self.trash_slot = 3
|
||
self.waste_liquid_slot = 6
|
||
|
||
elif product_name == "PRCXI9320":
|
||
self.rows = 3
|
||
self.columns = 4
|
||
self.layout = [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16]
|
||
self.trash_slot = 16
|
||
self.waste_liquid_slot = 12
|
||
self.default_layout = {"MatrixId":f"{time.time()}","MatrixName":f"{time.time()}","MatrixCount":16,"WorkTablets":
|
||
[{"Number": 1, "Code": "T1", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 2, "Code": "T2", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 3, "Code": "T3", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 4, "Code": "T4", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 5, "Code": "T5", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 6, "Code": "T6", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 7, "Code": "T7", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 8, "Code": "T8", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 9, "Code": "T9", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 10, "Code": "T10", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 11, "Code": "T11", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 12, "Code": "T12", "Material": {"uuid": "730067cf07ae43849ddf4034299030e9", "materialEnum": 0}}, # 这个设置成废液槽，用储液槽表示
|
||
{"Number": 13, "Code": "T13", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 14, "Code": "T14", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 15, "Code": "T15", "Material": {"uuid": "57b1e4711e9e4a32b529f3132fc5931f", "materialEnum": 0}},
|
||
{"Number": 16, "Code": "T16", "Material": {"uuid": "730067cf07ae43849ddf4034299030e9", "materialEnum": 0}} # 这个设置成垃圾桶，用储液槽表示
|
||
]
|
||
}
|
||
|
||
def get_layout(self) -> Dict[str, Any]:
|
||
return {
|
||
"rows": self.rows,
|
||
"columns": self.columns,
|
||
"layout": self.layout,
|
||
"trash_slot": self.trash_slot,
|
||
"waste_liquid_slot": self.waste_liquid_slot
|
||
}
|
||
|
||
def get_trash_slot(self) -> int:
|
||
return self.trash_slot
|
||
|
||
def get_waste_liquid_slot(self) -> int:
|
||
return self.waste_liquid_slot
|
||
|
||
def add_lab_resource(self, material_info):
|
||
self.labresource = material_info
|
||
|
||
def recommend_layout(self, needs: Dict[str, int]) -> Dict[str, Any]:
|
||
"""根据 needs 推荐布局"""
|
||
for k, v in needs.items():
|
||
if k not in self.labresource:
|
||
raise ValueError(f"Material {k} not found in lab resources.")
|
||
|
||
# 预留位置12和16不动
|
||
reserved_positions = {12, 16}
|
||
available_positions = [i for i in range(1, 17) if i not in reserved_positions]
|
||
|
||
# 计算总需求
|
||
total_needed = sum(needs.values())
|
||
if total_needed > len(available_positions):
|
||
raise ValueError(f"需要 {total_needed} 个位置，但只有 {len(available_positions)} 个可用位置（排除位置12和16）")
|
||
|
||
# 依次分配位置
|
||
current_pos = 0
|
||
for material_name, count in needs.items():
|
||
material_uuid = self.labresource[material_name]['uuid']
|
||
material_enum = self.labresource[material_name]['materialEnum']
|
||
|
||
for _ in range(count):
|
||
if current_pos >= len(available_positions):
|
||
raise ValueError("位置不足，无法分配更多物料")
|
||
|
||
position = available_positions[current_pos]
|
||
# 找到对应的tablet并更新
|
||
for tablet in self.default_layout['WorkTablets']:
|
||
if tablet['Number'] == position:
|
||
tablet['Material']['uuid'] = material_uuid
|
||
tablet['Material']['materialEnum'] = material_enum
|
||
break
|
||
|
||
current_pos += 1
|
||
|
||
return self.default_layout
|
||
|
||
|
||
if __name__ == "__main__":
|
||
with open("prcxi_material.json", "r") as f:
|
||
material_info = json.load(f)
|
||
|
||
layout = DefaultLayout("PRCXI9320")
|
||
layout.add_lab_resource(material_info)
|
||
plan = layout.recommend_layout({
|
||
"10μL加长 Tip头": 2,
|
||
"300μL Tip头": 2,
|
||
"96深孔板": 2,
|
||
})
|
||
|
||
|
||
|
||
|
||
# if __name__ == "__main__":
|
||
# # ---- 资源：SUP 供液（X），中间板 R1（4 孔空），目标板 R2（4 孔空）----
|
||
# # sup = MaterialResource("SUP", slot=5, well=[1], liquid_id="X", volume=10000)
|
||
# # r1 = MaterialResource("R1", slot=6, well=[1,2,3,4,5,6,7,8])
|
||
# # r2 = MaterialResource("R2", slot=7, well=[1,2,3,4,5,6,7,8])
|
||
|
||
# # pm = ProtocolManager()
|
||
# # # 步骤1：SUP -> R1，1->N 扇出，每孔 50 uL（总 200 uL）
|
||
# # pm.add_transfer(sup, r1, unit_volume=10.0)
|
||
# # # 步骤2：R1 -> R2，N->N 对应，每对 25 uL（总 100 uL；来自 R1 中已存在的混合物 X）
|
||
# # pm.add_transfer(r1, r2, unit_volume=120.0)
|
||
|
||
# # out = pm.compute_min_initials_with_tips()
|
||
# # # layout_planer = DefaultLayout('PRCXI9320')
|
||
# # # print(layout_planer.get_layout())
|
||
# # # print("回推最小需求：", out["liquid_setup"]) # {'SUP': {'X': 200.0}}
|
||
# # # print("步骤枪头建议：", out["step_tips"]) # [{'idx':0,'tip':'TIP_200uL','unit_volume':50.0}, {'idx':1,'tip':'TIP_50uL','unit_volume':25.0}]
|
||
|
||
# # # # 实际执行（可选）
|
||
# # # transfer_liquid(sup, r1, unit_volume=50.0)
|
||
# # # transfer_liquid(r1, r2, unit_volume=25.0)
|
||
# # # print("执行后 SUP：", sup.get_resource()) # 总体积 -200
|
||
# # # print("执行后 R1：", r1.get_resource()) # 每孔 25 uL（50 进 -25 出）
|
||
# # # print("执行后 R2：", r2.get_resource()) # 每孔 25 uL
|
||
|
||
|
||
# # from pylabrobot.resources.opentrons.tube_racks import *
|
||
# # from pylabrobot.resources.opentrons.plates import *
|
||
# # from pylabrobot.resources.opentrons.tip_racks import *
|
||
# # from pylabrobot.resources.opentrons.reservoirs import *
|
||
|
||
# # plate = [locals()['nest_96_wellplate_2ml_deep'](name="thermoscientificnunc_96_wellplate_2000ul"), locals()['corning_96_wellplate_360ul_flat'](name="corning_96_wellplate_360ul_flat")]
|
||
# # tiprack = [locals()['opentrons_96_tiprack_300ul'](name="opentrons_96_tiprack_300ul"), locals()['opentrons_96_tiprack_1000ul'](name="opentrons_96_tiprack_1000ul")]
|
||
# # trash = [locals()['axygen_1_reservoir_90ml'](name="axygen_1_reservoir_90ml")]
|
||
|
||
# # from pprint import pprint
|
||
|
||
# # lab_resource = LabResource()
|
||
# # lab_resource.add_tipracks(tiprack)
|
||
# # lab_resource.add_plates(plate)
|
||
# # lab_resource.add_trash(trash)
|
||
|
||
# # layout_planer = DefaultLayout('PRCXI9300')
|
||
# # layout_planer.add_lab_resource(lab_resource)
|
||
# # layout_planer.recommend_layout(out)
|
||
|
||
# with open("prcxi_material.json", "r") as f:
|
||
# material_info = json.load(f)
|
||
# # print("当前实验物料信息：", material_info)
|
||
|
||
# layout = DefaultLayout("PRCXI9320")
|
||
# layout.add_lab_resource(material_info)
|
||
# print(layout.default_layout['WorkTablets'])
|
||
# # plan = layout.recommend_layout({
|
||
# # "10μL加长 Tip头": 2,
|
||
# # "300μL Tip头": 2,
|
||
# # "96深孔板": 2,
|
||
# # })
|
||
|
||
|
||
|