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Update 9320

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Guangxin Zhang
2025-07-18 00:14:33 +08:00
parent f0de653e77
commit 9a8d50bbf3
2 changed files with 13274 additions and 31 deletions
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13245
unilabos/devices/liquid_handling/prcxi/deck.json Normal file
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File diff suppressed because it is too large Load Diff

60
unilabos/devices/liquid_handling/prcxi/prcxi.py
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@@ -448,7 +448,7 @@ class PRCXI9300Backend(LiquidHandlerBackend):
PlateNo = plate_indexes[0] + 1 PlateNo = plate_indexes[0] + 1
hole_col = tip_columns[0] + 1 hole_col = tip_columns[0] + 1
if self.channel_num == 1: if self._num_channels == 1:
hole_row = tipspot_index % 8 + 1 hole_row = tipspot_index % 8 + 1
step = self.api_client.Load(dosage=0, plate_no=PlateNo, is_whole_plate=False, hole_row=hole_row, hole_col=hole_col, step = self.api_client.Load(dosage=0, plate_no=PlateNo, is_whole_plate=False, hole_row=hole_row, hole_col=hole_col,
@@ -459,29 +459,6 @@ class PRCXI9300Backend(LiquidHandlerBackend):
async def drop_tips(self, ops: List[Drop], use_channels: List[int] = None): async def drop_tips(self, ops: List[Drop], use_channels: List[int] = None):
"""Pick up tips from the specified resource.""" """Pick up tips from the specified resource."""
plate = ops[0].resource.parent.parent
deck = plate.parent
plate_index = deck.children.index(plate)
if deck.children[plate_index].name == "trash":
step = self.api_client.UnLoad(
dosage=0,
plate_no=plate_index+1,
is_whole_plate=False,
hole_row=1,
hole_col=2,# 强制投放第二列了
blending_times=0,
balance_height=0,
plate_or_hole=f"H{hole_col}-8,T{PlateNo}",
hole_numbers="1,2,3,4,5,6,7,8",
)
self.steps_todo_list.append(step)
return
if len(ops) != 8:
raise ValueError(f"PRCXI9300Backend drop_tips: Expected 8 pickups, got {len(ops)}")
plate_indexes = [] plate_indexes = []
for op in ops: for op in ops:
plate = op.resource.parent.parent plate = op.resource.parent.parent
@@ -502,11 +479,29 @@ class PRCXI9300Backend(LiquidHandlerBackend):
PlateNo = plate_indexes[0] + 1 PlateNo = plate_indexes[0] + 1
hole_col = tip_columns[0] + 1 hole_col = tip_columns[0] + 1
if deck.children[plate_index].name == "trash":
step = self.api_client.UnLoad(
dosage=0,
plate_no=PlateNo,
is_whole_plate=False,
hole_row=3,
hole_col=3,
blending_times=0,
balance_height=0,
plate_or_hole=f"H{hole_col}-8,T{PlateNo}",
hole_numbers="1,2,3,4,5,6,7,8",
)
self.steps_todo_list.append(step)
return
if self.channel_num == 1:
hole_row = tipspot_index % 8 + 1
step = self.api_client.UnLoad( step = self.api_client.UnLoad(
dosage=0, dosage=0,
plate_no=PlateNo, plate_no=PlateNo,
is_whole_plate=False, is_whole_plate=False,
hole_row=1, hole_row=hole_row,
hole_col=hole_col, hole_col=hole_col,
blending_times=0, blending_times=0,
balance_height=0, balance_height=0,
@@ -1179,7 +1174,7 @@ if __name__ == "__main__":
"uuid": "04211a2dc93547fe9bf6121eac533650" "uuid": "04211a2dc93547fe9bf6121eac533650"
} }
}) })
plate8 = get_well_container("RackT8") plate8 = get_tip_rack("RackT8")
plate8.load_state({ plate8.load_state({
"Material": { "Material": {
"uuid": "068b3815e36b4a72a59bae017011b29f", "uuid": "068b3815e36b4a72a59bae017011b29f",
@@ -1231,7 +1226,10 @@ if __name__ == "__main__":
# deck.assign_child_resource(plate12, location=Coordinate(0, 0, 0)) # deck.assign_child_resource(plate12, location=Coordinate(0, 0, 0))
# deck.assign_child_resource(plate13, location=Coordinate(0, 0, 0)) # deck.assign_child_resource(plate13, location=Coordinate(0, 0, 0))
handler = PRCXI9300Handler(deck=deck, host="10.181.102.13", port=9999, timeout=10.0, setup=False, debug=True, matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1") handler = PRCXI9300Handler(deck=deck, host="10.181.102.13", port=9999,
timeout=10.0, setup=False, debug=True,
matrix_id="fd383e6d-2d0e-40b5-9c01-1b2870b1f1b1",
channel_num=1)
handler.set_tiprack([plate8]) # Set the tip rack for the handler handler.set_tiprack([plate8]) # Set the tip rack for the handler
asyncio.run(handler.setup()) # Initialize the handler and setup the connection asyncio.run(handler.setup()) # Initialize the handler and setup the connection
from pylabrobot.resources import set_volume_tracking from pylabrobot.resources import set_volume_tracking
@@ -1247,10 +1245,10 @@ if __name__ == "__main__":
json.dump(A, f, indent=4, ensure_ascii=False) json.dump(A, f, indent=4, ensure_ascii=False)
asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
asyncio.run(handler.pick_up_tips(plate8.children[3],[1])) asyncio.run(handler.pick_up_tips([plate8.children[3]],[0]))
asyncio.run(handler.aspirate(plate11.children[1],[10], [1])) asyncio.run(handler.aspirate([plate11.children[2]],[10], [0]))
asyncio.run(handler.dispense(plate1.children[3],[10],[1])) asyncio.run(handler.dispense([plate1.children[3]],[10],[0]))
asyncio.run(handler.mix(plate1.children[3], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100)) asyncio.run(handler.mix([plate1.children[3]], mix_time=3, mix_vol=5, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
asyncio.run(handler.discard_tips()) asyncio.run(handler.discard_tips())
# asyncio.run(handler.discard_tips()) # asyncio.run(handler.discard_tips())