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Refactor PRCXI9300Deck initialization and update plate configurations

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- Changed deck name from "PRCXI_Deck" to "PRCXI_Deck_9300".
- Updated plate4 initialization to use get_well_container instead of get_tip_rack.
- Modified plate4 material details with new UUID, code, and name.
- Renamed output JSON file to "deck_9300_new.json".
- Uncommented and adjusted liquid handling operations for clarity and future use.
This commit is contained in:
Guangxin Zhang
2025-07-19 21:07:17 +08:00
parent 81769d5a32
commit 03e8c1e46d
2 changed files with 2183 additions and 2279 deletions
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4360
test/experiments/prcxi_9300.json
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102
unilabos/devices/liquid_handling/prcxi/prcxi.py
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@@ -976,7 +976,7 @@ if __name__ == "__main__":
# 4.
deck = PRCXI9300Deck(name="PRCXI_Deck", size_x=100, size_y=100, size_z=100)
deck = PRCXI9300Deck(name="PRCXI_Deck_9300", size_x=100, size_y=100, size_z=100)
from pylabrobot.resources.opentrons.tip_racks import opentrons_96_tiprack_300ul,opentrons_96_tiprack_10ul
from pylabrobot.resources.opentrons.plates import corning_96_wellplate_360ul_flat, nest_96_wellplate_2ml_deep
@@ -1027,12 +1027,12 @@ if __name__ == "__main__":
}
})
plate4 = get_tip_rack("RackT4")
plate4 = get_well_container("PlateT4")
plate4.load_state({
"Material": {
"uuid": "076250742950465b9d6ea29a225dfb00",
"Code": "ZX-001-300",
"Name": "300μL Tip头"
"uuid": "57b1e4711e9e4a32b529f3132fc5931f",
"Code": "ZX-019-2.2",
"Name": "96深孔板"
}
})
@@ -1079,39 +1079,39 @@ if __name__ == "__main__":
set_volume_tracking(enabled=True)
from unilabos.resources.graphio import *
A = tree_to_list([resource_plr_to_ulab(deck)])
with open("deck.json", "w", encoding="utf-8") as f:
with open("deck_9300_new.json", "w", encoding="utf-8") as f:
json.dump(A, f, indent=4, ensure_ascii=False)
asyncio.run(handler.create_protocol(protocol_name="Test Protocol")) # Initialize the backend and setup the connection
asyncio.run(handler.pick_up_tips(plate1.children[:8],[0,1,2,3,4,5,6,7]))
print(plate1.children[:8])
asyncio.run(handler.aspirate(plate2.children[:8],[50]*8, [0,1,2,3,4,5,6,7]))
print(plate2.children[:8])
asyncio.run(handler.dispense(plate5.children[:8],[50]*8,[0,1,2,3,4,5,6,7]))
print(plate5.children[:8])
# asyncio.run(handler.pick_up_tips(plate1.children[:8],[0,1,2,3,4,5,6,7]))
# print(plate1.children[:8])
# asyncio.run(handler.aspirate(plate2.children[:8],[50]*8, [0,1,2,3,4,5,6,7]))
# print(plate2.children[:8])
# asyncio.run(handler.dispense(plate5.children[:8],[50]*8,[0,1,2,3,4,5,6,7]))
# print(plate5.children[:8])
# # # asyncio.run(handler.drop_tips(tip_rack.children[8:16],[0,1,2,3,4,5,6,7]))
asyncio.run(handler.discard_tips())
# # # # asyncio.run(handler.drop_tips(tip_rack.children[8:16],[0,1,2,3,4,5,6,7]))
# asyncio.run(handler.discard_tips())
# asyncio.run(handler.mix(well_containers.children[:8
# ], mix_time=3, mix_vol=50, height_to_bottom=0.5, offsets=Coordinate(0, 0, 0), mix_rate=100))
# #print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2)) # Print matrix info
# asyncio.run(handler.add_liquid(
# asp_vols=[100]*16,
# dis_vols=[100]*16,
# reagent_sources=plate2.children[:16],
# targets=plate5.children[:16],
# use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
# flow_rates=[None] * 32,
# offsets=[Coordinate(0, 0, 0)] * 32,
# liquid_height=[None] * 16,
# blow_out_air_volume=[None] * 16,
# delays=None,
# mix_time=3,
# mix_vol=50,
# spread="wide",
# ))
asyncio.run(handler.add_liquid(
asp_vols=[100]*16,
dis_vols=[100]*16,
reagent_sources=plate2.children[:16],
targets=plate5.children[:16],
use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
flow_rates=[None] * 32,
offsets=[Coordinate(0, 0, 0)] * 32,
liquid_height=[None] * 16,
blow_out_air_volume=[None] * 16,
delays=None,
mix_time=3,
mix_vol=50,
spread="wide",
))
asyncio.run(handler.run_protocol()) # Run the protocol
# asyncio.run(handler.remove_liquid(
# vols=[100]*16,
# sources=plate2.children[-16:],
@@ -1124,27 +1124,27 @@ if __name__ == "__main__":
# spread="wide",
# ))
acid = [20]*8+[40]*8+[60]*8+[80]*8+[100]*8+[120]*8+[140]*8+[160]*8+[180]*8+[200]*8+[220]*8+[240]*8
alkaline = acid[::-1] # Reverse the acid list for alkaline
asyncio.run(handler.transfer_liquid(
asp_vols=acid,
dis_vols=acid,
tip_racks=[plate1],
sources=plate2.children[:],
targets=plate5.children[:],
use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
offsets=[Coordinate(0, 0, 0)] * 32,
asp_flow_rates=[None] * 16,
dis_flow_rates=[None] * 16,
liquid_height=[None] * 32,
blow_out_air_volume=[None] * 32,
mix_times=3,
mix_vol=50,
spread="wide",
))
print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2)) # Print matrix info
# # input("pick_up_tips add step")
asyncio.run(handler.run_protocol()) # Run the protocol
# acid = [20]*8+[40]*8+[60]*8+[80]*8+[100]*8+[120]*8+[140]*8+[160]*8+[180]*8+[200]*8+[220]*8+[240]*8
# alkaline = acid[::-1] # Reverse the acid list for alkaline
# asyncio.run(handler.transfer_liquid(
# asp_vols=acid,
# dis_vols=acid,
# tip_racks=[plate1],
# sources=plate2.children[:],
# targets=plate5.children[:],
# use_channels=[0, 1, 2, 3, 4, 5, 6, 7],
# offsets=[Coordinate(0, 0, 0)] * 32,
# asp_flow_rates=[None] * 16,
# dis_flow_rates=[None] * 16,
# liquid_height=[None] * 32,
# blow_out_air_volume=[None] * 32,
# mix_times=3,
# mix_vol=50,
# spread="wide",
# ))
# print(json.dumps(handler._unilabos_backend.steps_todo_list, indent=2)) # Print matrix info
# # # input("pick_up_tips add step")
# asyncio.run(handler.run_protocol()) # Run the protocol
# # input("Running protocol...")
# # input("Press Enter to continue...") # Wait for user input before proceeding
# # print("PRCXI9300Handler initialized with deck and host settings.")