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Update biomek_test.py

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qxw138
2025-06-08 15:52:20 +08:00
parent c0b7f2decd
commit 9b3377aedb
1 changed files with 442 additions and 446 deletions
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unilabos/devices/liquid_handling/biomek_test.py
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@@ -276,14 +276,14 @@ class LiquidHandlerBiomek:
"Class": f"LabwareClasses\\{class_name}",
"DataSets": {"Volume": {}},
"RuntimeDataSets": {"Volume": {}},
"EvalAmounts": (float(liquid_volume[0]),) if liquid_volume else (0.0,),
"EvalAmounts": (float(liquid_volume[0]),) if liquid_volume else (0,),
"Nominal": False,
"EvalLiquids": (liquid_type[0],) if liquid_type else ("Water",)
}
elif instrument_type == "plate_96":
# 96孔板类型配置
volume_per_well = float(liquid_volume[0]) if liquid_volume else 500.0
volume_per_well = float(liquid_volume[0]) if liquid_volume else 0
liquid_per_well = liquid_type[0] if liquid_type else "Water"
config = {
@@ -473,452 +473,448 @@ if __name__ == "__main__":
print("包含: 仪器设置 + 完整实验步骤")
# 完整的步骤信息（从biomek.py复制）
steps_info = '''
steps_info = {
"steps": [
{
"steps": [
{
"step_number": 1,
"operation": "transfer",
"description": "转移PCR产物或酶促反应液至0.05ml 96孔板中",
"parameters": {
"source": "P1",
"target": "P11",
"tip_rack": "BC230",
"volume": 50
}
},
{
"step_number": 2,
"operation": "transfer",
"description": "加入2倍体积Bind Beads BC至产物中",
"parameters": {
"source": "P2",
"target": "P11",
"tip_rack": "BC230",
"volume": 100
}
},
{
"step_number": 3,
"operation": "move_labware",
"description": "移动P11至Orbital1用于振荡混匀",
"parameters": {
"source": "P11",
"target": "Orbital1"
}
},
{
"step_number": 4,
"operation": "oscillation",
"description": "在Orbital1上振荡混匀Bind Beads BC与PCR产物（700-900rpm，300秒）",
"parameters": {
"rpm": 800,
"time": 300
}
},
{
"step_number": 5,
"operation": "move_labware",
"description": "移动混匀后的板回P11",
"parameters": {
"source": "Orbital1",
"target": "P11"
}
},
{
"step_number": 6,
"operation": "move_labware",
"description": "将P11移动到磁力架（P12）吸附3分钟",
"parameters": {
"source": "P11",
"target": "P12"
}
},
{
"step_number": 7,
"operation": "incubation",
"description": "磁力架上室温静置3分钟完成吸附",
"parameters": {
"time": 180
}
},
{
"step_number": 8,
"operation": "transfer",
"description": "去除上清液至废液槽",
"parameters": {
"source": "P12",
"target": "P22",
"tip_rack": "BC230",
"volume": 150
}
},
{
"step_number": 9,
"operation": "transfer",
"description": "加入300-500μl 75%乙醇清洗",
"parameters": {
"source": "P3",
"target": "P12",
"tip_rack": "BC230",
"volume": 400
}
},
{
"step_number": 10,
"operation": "move_labware",
"description": "移动清洗板到Orbital1进行振荡",
"parameters": {
"source": "P12",
"target": "Orbital1"
}
},
{
"step_number": 11,
"operation": "oscillation",
"description": "乙醇清洗液振荡混匀（700-900rpm, 45秒）",
"parameters": {
"rpm": 800,
"time": 45
}
},
{
"step_number": 12,
"operation": "move_labware",
"description": "振荡后将板移回磁力架P12吸附",
"parameters": {
"source": "Orbital1",
"target": "P12"
}
},
{
"step_number": 13,
"operation": "incubation",
"description": "吸附3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 14,
"operation": "transfer",
"description": "去除乙醇上清液至废液槽",
"parameters": {
"source": "P12",
"target": "P22",
"tip_rack": "BC230",
"volume": 400
}
},
{
"step_number": 15,
"operation": "transfer",
"description": "第二次加入300-500μl 75%乙醇清洗",
"parameters": {
"source": "P3",
"target": "P12",
"tip_rack": "BC230",
"volume": 400
}
},
{
"step_number": 16,
"operation": "move_labware",
"description": "再次移动清洗板到Orbital1振荡",
"parameters": {
"source": "P12",
"target": "Orbital1"
}
},
{
"step_number": 17,
"operation": "oscillation",
"description": "再次乙醇清洗液振荡混匀（700-900rpm, 45秒）",
"parameters": {
"rpm": 800,
"time": 45
}
},
{
"step_number": 18,
"operation": "move_labware",
"description": "振荡后板送回磁力架P12吸附",
"parameters": {
"source": "Orbital1",
"target": "P12"
}
},
{
"step_number": 19,
"operation": "incubation",
"description": "再次吸附3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 20,
"operation": "transfer",
"description": "去除乙醇上清液至废液槽",
"parameters": {
"source": "P12",
"target": "P22",
"tip_rack": "BC230",
"volume": 400
}
},
{
"step_number": 21,
"operation": "incubation",
"description": "空气干燥15分钟",
"parameters": {
"time": 900
}
},
{
"step_number": 22,
"operation": "transfer",
"description": "加30-50μl Elution Buffer洗脱",
"parameters": {
"source": "P4",
"target": "P12",
"tip_rack": "BC230",
"volume": 40
}
},
{
"step_number": 23,
"operation": "move_labware",
"description": "移动到Orbital1振荡混匀（60秒）",
"parameters": {
"source": "P12",
"target": "Orbital1"
}
},
{
"step_number": 24,
"operation": "oscillation",
"description": "Elution Buffer振荡混匀（700-900rpm, 60秒）",
"parameters": {
"rpm": 800,
"time": 60
}
},
{
"step_number": 25,
"operation": "move_labware",
"description": "振荡后送回磁力架P12",
"parameters": {
"source": "Orbital1",
"target": "P12"
}
},
{
"step_number": 26,
"operation": "incubation",
"description": "室温静置3分钟（洗脱反应）",
"parameters": {
"time": 180
}
},
{
"step_number": 27,
"operation": "transfer",
"description": "将上清液（DNA）转移到新板（P13）",
"parameters": {
"source": "P12",
"target": "P13",
"tip_rack": "BC230",
"volume": 40
}
}
]
"step_number": 1,
"operation": "transfer",
"description": "转移PCR产物或酶促反应液至0.5ml 96孔板中",
"parameters": {
"source": "P1",
"target": "P11",
"tip_rack": "BC230",
"volume": 50
}
},
{
"step_number": 2,
"operation": "transfer",
"description": "加入2倍体积的Bind Beads BC至产物中",
"parameters": {
"source": "P2",
"target": "P11",
"tip_rack": "BC230",
"volume": 100
}
},
{
"step_number": 3,
"operation": "oscillation",
"description": "振荡混匀300秒",
"parameters": {
"rpm": 800,
"time": 300
}
},
{
"step_number": 4,
"operation": "move_labware",
"description": "转移至96孔磁力架上吸附3分钟",
"parameters": {
"source": "P11",
"target": "P12"
}
},
{
"step_number": 5,
"operation": "incubation",
"description": "吸附3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 6,
"operation": "transfer",
"description": "吸弃或倒除上清液",
"parameters": {
"source": "P12",
"target": "P22",
"tip_rack": "BC230",
"volume": 150
}
},
{
"step_number": 7,
"operation": "transfer",
"description": "加入300-500μl 75%乙醇",
"parameters": {
"source": "P3",
"target": "P12",
"tip_rack": "BC230",
"volume": 400
}
},
{
"step_number": 8,
"operation": "move_labware",
"description": "移动至振荡器进行振荡混匀",
"parameters": {
"source": "P12",
"target": "Orbital1"
}
},
{
"step_number": 9,
"operation": "oscillation",
"description": "振荡混匀60秒",
"parameters": {
"rpm": 800,
"time": 60
}
},
{
"step_number": 10,
"operation": "move_labware",
"description": "转移至96孔磁力架上吸附3分钟",
"parameters": {
"source": "Orbital1",
"target": "P12"
}
},
{
"step_number": 11,
"operation": "incubation",
"description": "吸附3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 12,
"operation": "transfer",
"description": "吸弃或倒弃废液",
"parameters": {
"source": "P12",
"target": "P22",
"tip_rack": "BC230",
"volume": 400
}
},
{
"step_number": 13,
"operation": "transfer",
"description": "重复加入75%乙醇",
"parameters": {
"source": "P3",
"target": "P12",
"tip_rack": "BC230",
"volume": 400
}
},
{
"step_number": 14,
"operation": "move_labware",
"description": "移动至振荡器进行振荡混匀",
"parameters": {
"source": "P12",
"target": "Orbital1"
}
},
{
"step_number": 15,
"operation": "oscillation",
"description": "振荡混匀60秒",
"parameters": {
"rpm": 800,
"time": 60
}
},
{
"step_number": 16,
"operation": "move_labware",
"description": "转移至96孔磁力架上吸附3分钟",
"parameters": {
"source": "Orbital1",
"target": "P12"
}
},
{
"step_number": 17,
"operation": "incubation",
"description": "吸附3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 18,
"operation": "transfer",
"description": "吸弃或倒弃废液",
"parameters": {
"source": "P12",
"target": "P22",
"tip_rack": "BC230",
"volume": 400
}
},
{
"step_number": 19,
"operation": "move_labware",
"description": "正放96孔板，空气干燥15分钟",
"parameters": {
"source": "P12",
"target": "P13"
}
},
{
"step_number": 20,
"operation": "incubation",
"description": "空气干燥15分钟",
"parameters": {
"time": 900
}
},
{
"step_number": 21,
"operation": "transfer",
"description": "加入30-50μl Elution Buffer",
"parameters": {
"source": "P4",
"target": "P13",
"tip_rack": "BC230",
"volume": 40
}
},
{
"step_number": 22,
"operation": "move_labware",
"description": "移动至振荡器进行振荡混匀",
"parameters": {
"source": "P13",
"target": "Orbital1"
}
},
{
"step_number": 23,
"operation": "oscillation",
"description": "振荡混匀60秒",
"parameters": {
"rpm": 800,
"time": 60
}
},
{
"step_number": 24,
"operation": "move_labware",
"description": "室温静置3分钟",
"parameters": {
"source": "Orbital1",
"target": "P13"
}
},
{
"step_number": 25,
"operation": "incubation",
"description": "室温静置3分钟",
"parameters": {
"time": 180
}
},
{
"step_number": 26,
"operation": "move_labware",
"description": "转移至96孔磁力架上吸附2分钟",
"parameters": {
"source": "P13",
"target": "P12"
}
},
{
"step_number": 27,
"operation": "incubation",
"description": "吸附2分钟",
"parameters": {
"time": 120
}
},
{
"step_number": 28,
"operation": "transfer",
"description": "将DNA转移至新的板中",
"parameters": {
"source": "P12",
"target": "P14",
"tip_rack": "BC230",
"volume": 40
}
}
'''
# 完整的labware配置信息（从biomek.py复制）
labware_with_liquid = '''
[
{
"id": "Tip Rack BC230 on TL1",
"parent": "deck",
"slot_on_deck": "TL1",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on TL2",
"parent": "deck",
"slot_on_deck": "TL2",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on TL3",
"parent": "deck",
"slot_on_deck": "TL3",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on TL4",
"parent": "deck",
"slot_on_deck": "TL4",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on TL5",
"parent": "deck",
"slot_on_deck": "TL5",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on P5",
"parent": "deck",
"slot_on_deck": "P5",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on P6",
"parent": "deck",
"slot_on_deck": "P6",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on P15",
"parent": "deck",
"slot_on_deck": "P15",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 on P16",
"parent": "deck",
"slot_on_deck": "P16",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "stock plate on P1",
"parent": "deck",
"slot_on_deck": "P1",
"class_name": "nest_12_reservoir_15ml",
"liquid_type": [
"master_mix"
],
"liquid_volume": [10000],
"liquid_input_wells": [
"A1"
]
},
{
"id": "stock plate on P2",
"parent": "deck",
"slot_on_deck": "P2",
"class_name": "nest_12_reservoir_15ml",
"liquid_type": [
"bind beads"
],
"liquid_volume": [10000],
"liquid_input_wells": [
"A1"
]
},
{
"id": "stock plate on P3",
"parent": "deck",
"slot_on_deck": "P3",
"class_name": "nest_12_reservoir_15ml",
"liquid_type": [
"ethyl alcohol"
],
"liquid_volume": [10000],
"liquid_input_wells": [
"A1"
]
},
{
"id": "elution buffer on P4",
"parent": "deck",
"slot_on_deck": "P4",
"class_name": "nest_12_reservoir_15ml",
"liquid_type": [
"elution buffer"
],
"liquid_volume": [5000],
"liquid_input_wells": [
"A1"
]
},
{
"id": "oscillation",
"parent": "deck",
"slot_on_deck": "Orbital1",
"class_name": "Orbital",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P11",
"parent": "deck",
"slot_on_deck": "P11",
"class_name": "NEST 2ml Deep Well Plate",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "magnetics module on P12",
"parent": "deck",
"slot_on_deck": "P12",
"class_name": "magnetics module",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P13",
"parent": "deck",
"slot_on_deck": "P13",
"class_name": "NEST 2ml Deep Well Plate",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "waste on P22",
"parent": "deck",
"slot_on_deck": "P22",
"class_name": "nest_1_reservoir_195ml",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
}
]
'''
]
}
# 完整的labware配置信息
labware_with_liquid = [
{
"id": "Tip Rack BC230 TL1",
"parent": "deck",
"slot_on_deck": "TL1",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 TL2",
"parent": "deck",
"slot_on_deck": "TL2",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 TL3",
"parent": "deck",
"slot_on_deck": "TL3",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 TL4",
"parent": "deck",
"slot_on_deck": "TL4",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 TL5",
"parent": "deck",
"slot_on_deck": "TL5",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 P5",
"parent": "deck",
"slot_on_deck": "P5",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 P6",
"parent": "deck",
"slot_on_deck": "P6",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 P15",
"parent": "deck",
"slot_on_deck": "P15",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "Tip Rack BC230 P16",
"parent": "deck",
"slot_on_deck": "P16",
"class_name": "BC230",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "stock plate on P1",
"parent": "deck",
"slot_on_deck": "P1",
"class_name": "AgilentReservoir",
"liquid_type": ["PCR product"],
"liquid_volume": [5000],
"liquid_input_wells": ["A1"]
},
{
"id": "stock plate on P2",
"parent": "deck",
"slot_on_deck": "P2",
"class_name": "AgilentReservoir",
"liquid_type": ["bind beads"],
"liquid_volume": [100000],
"liquid_input_wells": ["A1"]
},
{
"id": "stock plate on P3",
"parent": "deck",
"slot_on_deck": "P3",
"class_name": "AgilentReservoir",
"liquid_type": ["75% ethanol"],
"liquid_volume": [100000],
"liquid_input_wells": ["A1"]
},
{
"id": "stock plate on P4",
"parent": "deck",
"slot_on_deck": "P4",
"class_name": "AgilentReservoir",
"liquid_type": ["Elution Buffer"],
"liquid_volume": [5000],
"liquid_input_wells": ["A1"]
},
{
"id": "working plate on P11",
"parent": "deck",
"slot_on_deck": "P11",
"class_name": "BCDeep96Round",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P12",
"parent": "deck",
"slot_on_deck": "P12",
"class_name": "BCDeep96Round",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P13",
"parent": "deck",
"slot_on_deck": "P13",
"class_name": "BCDeep96Round",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "working plate on P14",
"parent": "deck",
"slot_on_deck": "P14",
"class_name": "BCDeep96Round",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "waste on P22",
"parent": "deck",
"slot_on_deck": "P22",
"class_name": "AgilentReservoir",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
},
{
"id": "oscillation",
"parent": "deck",
"slot_on_deck": "Orbital1",
"class_name": "Orbital",
"liquid_type": [],
"liquid_volume": [],
"liquid_input_wells": []
}
]
# 创建handler实例
handler = LiquidHandlerBiomek()